EC: 3.2.1.25

beta-mannosidase (beta-D-mannoside mannohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q9UUZ3 [beta-mannosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_mannopyranoside -> p_Nitrophenol + beta_D_Mannose		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Citrate-phosphate
PH: 4.5
Temperature: 40
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

H2O + Cellopentaose -> D_Glucose + Cellotetraose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

H2O + Cellohexaose -> Cellopentaose + D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

Laminaritriose + H2O -> beta_D_Glucose + Laminaribiose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

Laminaribiose + H2O -> 2 beta_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

H2O + Cellotetraose -> D_Glucose + Cellotriose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

Cellotriose + H2O -> D_Glucose + Cellobiose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

H2O + p_Nitrophenyl_beta_D_glucoside -> beta_D_Glucose + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

Cellobiose + H2O -> 2 beta_D_Glucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:B5A496 [beta-mannosidase(Enzyme) wildtype rHvBII]

H2O + p_Nitrophenyl_beta_D_mannopyranoside -> p_Nitrophenol + beta_D_Mannose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Sodium acetate
PH: 4
Temperature: 30
uniprot:Q4FZV0 [beta-mannosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_mannoside -> beta_D_Mannose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q4FZV0 [beta-mannosidase(Enzyme) wildtype]

p_Nitrophenyl_beta_D_mannoside + H2O -> beta_D_Mannose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q4FZV0 [beta-mannosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_beta_D_mannoside -> p_Nitrophenol + beta_D_Mannose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q4FZV0 [beta-mannosidase(Enzyme) wildtype]

p_Nitrophenyl_beta_D_mannoside + H2O -> p_Nitrophenol + beta_D_Mannose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
uniprot:Q4FZV0 [beta-mannosidase(Enzyme) wildtype]

p_Nitrophenyl_beta_D_mannoside + H2O -> beta_D_Mannose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37

Pathways

pathway id name
Reactome:R-MMU-1430728 Metabolism
Reactome:R-CEL-71387 Carbohydrate metabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-CEL-8853383 Lysosomal oligosaccharide catabolism
Reactome:R-HSA-8853383 Lysosomal oligosaccharide catabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-8853383 Lysosomal oligosaccharide catabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-RNO-8853383 Lysosomal oligosaccharide catabolism