EC: 1.1.1.145

3beta-hydroxy-DELTA5-steroid dehydrogenase (3beta-hydroxy-DELTA5-steroid:NAD+ 3-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q9N119 [Enzyme wildtype]

NAD+ + 7alpha_Hydroxycholesterol -> 7alpha_Hydroxycholest_4_en_3_one + NADH + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM phosphate, 0.1 mM EDTA
PH: 7.4
Temperature: 37
uniprot:Q9N119 [Enzyme wildtype]

NAD+ + 7_alpha,27_Dihydroxycholesterol -> NADH + H+ + 7alpha,27_Dihydroxycholest_4_en_3_one		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM phosphate, 0.1 mM EDTA
PH: 7.4
Temperature: 37
uniprot:P14060 [3beta-hydroxy-Delta5-steroid dehydrogenase(Enzyme) wildtype]

3beta_Hydroxyandrost_5_en_17_one + NAD+ -> NADH + Androst_5_ene_3,17_dione		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.02 M potassium phosphate
PH: 7.4
Temperature: 27
uniprot:P14060 [3beta-hydroxy-Delta5-steroid dehydrogenase(Enzyme) mutant D265N]

3beta_Hydroxyandrost_5_en_17_one + NAD+ -> NADH + Androst_5_ene_3,17_dione		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.02 M potassium phosphate
PH: 7.4
Temperature: 27
uniprot:P14060 [3beta-hydroxy-Delta5-steroid dehydrogenase(Enzyme) wildtype]

NAD+ + 3beta_Hydroxyandrost_5_en_17_one -> NADH + Androst_5_ene_3,17_dione		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.02 M potassium phosphate
PH: 7.4
Temperature: 27
uniprot:P14060 [3beta-hydroxy-Delta5-steroid dehydrogenase(Enzyme) mutant D241N]

3beta_Hydroxyandrost_5_en_17_one + NAD+ -> Androst_5_ene_3,17_dione + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.02 M potassium phosphate
PH: 7.4
Temperature: 27
uniprot:P14060 [3beta-hydroxy-Delta5-steroid dehydrogenase(Enzyme) mutant D36A/K37R]

NADP+ + 3beta_Hydroxyandrost_5_en_17_one -> NADPH + Androst_5_ene_3,17_dione		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.02 M potassium phosphate
PH: 7.4
Temperature: 27
uniprot:P14060 [3beta-hydroxy-Delta5-steroid dehydrogenase(Enzyme) mutant D36A/K37R]

3beta_Hydroxyandrost_5_en_17_one + NADP+ -> NADPH + Androst_5_ene_3,17_dione		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.02 M potassium phosphate
PH: 7.4
Temperature: 27
uniprot:P14060 [3beta-hydroxy-Delta5-steroid dehydrogenase(Enzyme) mutant D241N]

3beta_Hydroxyandrost_5_en_17_one + NAD+ -> Androst_5_ene_3,17_dione + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.02 M potassium phosphate
PH: 7.4
Temperature: 27
uniprot:P14060 [3beta-hydroxy-Delta5-steroid dehydrogenase(Enzyme) mutant D265N]

NAD+ + 3beta_Hydroxyandrost_5_en_17_one -> Androst_5_ene_3,17_dione + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.02 M potassium phosphate
PH: 7.4
Temperature: 27

Pathways

pathway id name
BioCyc:HUMAN_PWY66-378 androgen biosynthesis
BioCyc:MTBH37RV_PWY-6946 cholesterol degradation to androstenedione II (cholesterol dehydrogenase)
BioCyc:MTBCDC1551_PWY-6943 testosterone and androsterone degradation to androstendione
BioCyc:META_PWY-6946 cholesterol degradation to androstenedione II (cholesterol dehydrogenase)
BioCyc:MTBCDC1551_PWY-6937 superpathway of testosterone and androsterone degradation
BioCyc:META_PWY66-378 androgen biosynthesis
BioCyc:MTBH37RV_PWY-6947 superpathway of cholesterol degradation II (cholesterol dehydrogenase)
BioCyc:MTBH37RV_PWY-6945 cholesterol degradation to androstenedione I (cholesterol oxidase)
BioCyc:MTBCDC1551_PWY-6947 superpathway of cholesterol degradation II (cholesterol dehydrogenase)
BioCyc:MTBCDC1551_PWY-6945 cholesterol degradation to androstenedione I (cholesterol oxidase)
BioCyc:HUMAN_PWY-7299 progesterone biosynthesis
BioCyc:META_PWY-7299 progesterone biosynthesis
BioCyc:META_PWY-6947 superpathway of cholesterol degradation II (cholesterol dehydrogenase)
BioCyc:META_PWY-6945 cholesterol degradation to androstenedione I (cholesterol oxidase)
BioCyc:MTBCDC1551_PWY-6946 cholesterol degradation to androstenedione II (cholesterol dehydrogenase)
BioCyc:MTBCDC1551_PWY-6948 sitosterol degradation to androstenedione
BioCyc:MTBCDC1551_PWY-6944 androstenedione degradation
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-194002 Glucocorticoid biosynthesis
Reactome:R-CFA-1430728 Metabolism
Reactome:R-CFA-556833 Metabolism of lipids
Reactome:R-CFA-194002 Glucocorticoid biosynthesis
Reactome:R-HSA-194002 Glucocorticoid biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-RNO-196071 Metabolism of steroid hormones
Reactome:R-RNO-193048 Androgen biosynthesis
Reactome:R-SSC-196071 Metabolism of steroid hormones
Reactome:R-SSC-193048 Androgen biosynthesis
Reactome:R-BTA-8957322 Metabolism of steroids
Reactome:R-CFA-8957322 Metabolism of steroids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-MMU-196071 Metabolism of steroid hormones
Reactome:R-MMU-193048 Androgen biosynthesis
Reactome:R-RNO-193993 Mineralocorticoid biosynthesis
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-556833 Metabolism of lipids
Reactome:R-SSC-193993 Mineralocorticoid biosynthesis
Reactome:R-BTA-193993 Mineralocorticoid biosynthesis
Reactome:R-CFA-193993 Mineralocorticoid biosynthesis
Reactome:R-HSA-193993 Mineralocorticoid biosynthesis
Reactome:R-MMU-194002 Glucocorticoid biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-SSC-8957322 Metabolism of steroids
Reactome:R-BTA-196071 Metabolism of steroid hormones
Reactome:R-BTA-193048 Androgen biosynthesis
Reactome:R-CFA-196071 Metabolism of steroid hormones
Reactome:R-CFA-193048 Androgen biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-196071 Metabolism of steroid hormones
Reactome:R-HSA-193048 Androgen biosynthesis
Reactome:R-MMU-193993 Mineralocorticoid biosynthesis
Reactome:R-RNO-194002 Glucocorticoid biosynthesis
Reactome:R-SSC-194002 Glucocorticoid biosynthesis
WikiPathways:WP5368 Sulfatase and aromatase pathway
WikiPathways:WP5320 Male steroid hormones in cardiomyocyte energy metabolism
WikiPathways:WP4524 Alternative pathway of fetal androgen synthesis
PathBank:SMP0121126 Aldosterone from Steroidogenesis
PathBank:SMP0000068 Androgen and Estrogen Metabolism
PathBank:SMP0000373 Adrenal Hyperplasia Type 3 or Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
PathBank:SMP0000576 21-Hydroxylase Deficiency (CYP21)
PathBank:SMP0000718 3-beta-Hydroxysteroid Dehydrogenase Deficiency
PathBank:SMP0000356 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0000575 11-beta-Hydroxylase Deficiency (CYP11B1)
PathBank:SMP0000717 Apparent Mineralocorticoid Excess Syndrome
PathBank:SMP0000130 Steroidogenesis
PathBank:SMP0000371 Congenital Lipoid Adrenal Hyperplasia (CLAH) or Lipoid CAH
PathBank:SMP0000565 Aromatase Deficiency
PathBank:SMP0000577 Corticosterone Methyl Oxidase I Deficiency (CMO I)
PathBank:SMP0000372 Adrenal Hyperplasia Type 5 or Congenital Adrenal Hyperplasia Due to 17 alpha-Hydroxylase Deficiency
PathBank:SMP0000566 17-alpha-Hydroxylase Deficiency (CYP17)
PathBank:SMP0000578 Corticosterone Methyl Oxidase II Deficiency (CMO II)