EC: 3.1.4.35

3',5'-cyclic-GMP phosphodiesterase (3',5'-cyclic-GMP 5'-nucleotidohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q54HY0 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM Hepes/NaOH, 1 mM EGTA, 5 mM MgCl2
PH: 7
Temperature: 22
uniprot:Q9Y233 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:B0G0Y8 [Mg2+]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Hepes/NaOH
PH: 7
Temperature: 22
- [Mg2+]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Hepes/NaOH, 5 mM DTT, 50 µM cAMP
PH: 7
Temperature: 22
uniprot:B0G0Y8 [Mg2+]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Hepes/NaOH
PH: 7
Temperature: 22
uniprot:B0G0Y8 [Mg2+]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Hepes/NaOH, 5 mM DTT, 50 µM cAMP
PH: 7
Temperature: 22
uniprot:B0G0Y8 [Mg2+]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Hepes/NaOH, 5 mM DTT, 50 µM cAMP
PH: 7
Temperature: 22
uniprot:Q8MLZ3 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype PDE5]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Hepes/NaOH, 0.5 mM EGTA, 0.25 M sucrose, 5 mM MgCl2
PH: 7
Temperature: 22
uniprot:Q8MM62 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype PDE6]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Hepes/NaOH, 0.5 mM EGTA, 0.25 M sucrose, 5 mM MgCl2
PH: 7
Temperature: 22
uniprot:O76083 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT
PH: 8.2
Temperature: 22
uniprot:O76083 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) mutant E406A]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT
PH: 8.2
Temperature: 22
uniprot:O76083 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) mutant Q453E]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT
PH: 8.2
Temperature: 22
uniprot:O76083 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT
PH: 8.2
Temperature: 22
uniprot:O76083 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) mutant E406A]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT
PH: 8.2
Temperature: 22
uniprot:O76083 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) mutant Q453E]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.5 mM DTT
PH: 8.2
Temperature: 22
uniprot:Q8CA95 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Mops, 0.8 mM EGTA, 15 mM Magnesium acetate, 0.2 mg/ml BSA
PH: 7.5
Temperature: 36
uniprot:Q9Y233 [Mg2+]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1.0 mM DTT, 10 mM MgCl2
PH: 7.5
Temperature: 22
uniprot:O70628 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM MOPS, 0.8 mM EGTA, 15.0 mM magnesium acetate, 0.2 mg/ml bovine serum albumin
PH: 7.5
Temperature: 36
uniprot:Q9HCR9 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype His-tagged splice variant PDE11A1]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9HCR9 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype His-tagged splice variant PDE11A2]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9HCR9 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype His-tagged splice variant PDE11A3]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9HCR9 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype His-tagged splice variant PDE11A4]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q8VID6 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) mutant His-tagged truncated PDE11At]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q8VID6 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype His-tagged splice variant PDE11A4]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q8VID6 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype His-tagged splice variant PDE11A2]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q8VID6 [3',5'-cyclic-GMP phosphodiesterase(Enzyme) wildtype His-tagged splice variant PDE11A3]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9HCR9 [Mg2+]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 4 mM 2-mercaptoethanol, 0.33 mg/ml BSA
PH: 8
Temperature: 37
uniprot:Q9HCR9 [Mg2+]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 4 mM 2-mercaptoethanol, 0.33 mg/ml BSA
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY3DJ-7 cyclic GMP biosynthesis
Reactome:R-BTA-9709957 Sensory Perception
Reactome:R-HSA-9709957 Sensory Perception
Reactome:R-CFA-9709957 Sensory Perception
Reactome:R-MMU-9709957 Sensory Perception
Reactome:R-BTA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-BTA-4086398 Ca2+ pathway
Reactome:R-CFA-162582 Signaling Pathways
Reactome:R-CFA-2187338 Visual phototransduction
Reactome:R-CFA-2514856 The phototransduction cascade
Reactome:R-HSA-4086398 Ca2+ pathway
Reactome:R-HSA-445355 Smooth Muscle Contraction
Reactome:R-MMU-445355 Smooth Muscle Contraction
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-MMU-4086398 Ca2+ pathway
Reactome:R-BTA-445355 Smooth Muscle Contraction
Reactome:R-BTA-162582 Signaling Pathways
Reactome:R-BTA-2187338 Visual phototransduction
Reactome:R-BTA-2485179 Activation of the phototransduction cascade
Reactome:R-BTA-3858494 Beta-catenin independent WNT signaling
Reactome:R-CFA-195721 Signaling by WNT
Reactome:R-DME-445355 Smooth Muscle Contraction
Reactome:R-HSA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-HSA-195721 Signaling by WNT
Reactome:R-HSA-3858494 Beta-catenin independent WNT signaling
Reactome:R-MMU-2187338 Visual phototransduction
Reactome:R-MMU-2485179 Activation of the phototransduction cascade
Reactome:R-MMU-3858494 Beta-catenin independent WNT signaling
Reactome:R-RNO-397014 Muscle contraction
Reactome:R-BTA-397014 Muscle contraction
Reactome:R-BTA-195721 Signaling by WNT
Reactome:R-CFA-2485179 Activation of the phototransduction cascade
Reactome:R-CFA-3858494 Beta-catenin independent WNT signaling
Reactome:R-HSA-2187338 Visual phototransduction
Reactome:R-HSA-2514856 The phototransduction cascade
Reactome:R-MMU-195721 Signaling by WNT
Reactome:R-RNO-445355 Smooth Muscle Contraction
Reactome:R-BTA-2514856 The phototransduction cascade
Reactome:R-CFA-4086398 Ca2+ pathway
Reactome:R-DME-397014 Muscle contraction
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-2485179 Activation of the phototransduction cascade
Reactome:R-HSA-397014 Muscle contraction
Reactome:R-MMU-397014 Muscle contraction
Reactome:R-MMU-2514856 The phototransduction cascade
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0087239 Purine Metabolism