EC: 3.1.2.4

3-hydroxyisobutyryl-CoA hydrolase (3-hydroxy-2-methylpropanoyl-CoA hydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q9LKJ1 [3-hydroxyisobutyryl-CoA hydrolase(Enzyme) wildtype GST-CHY1]

H2O + 3_Hydroxy_2_methylpropanoyl_CoA -> 3_Hydroxy_2_methylpropanoate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Phosphate, 50 mM NaCl, 1 mM EDTA, 0.1% Triton X-100
PH: 7.5
Temperature: 25
uniprot:Q5XIE6 [3-hydroxyisobutyryl-CoA hydrolase(Enzyme) wildtype]

3_Hydroxypropanoyl_CoA + H2O -> Coenzyme_A + 3_Hydroxypropanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl, 1 mM EDTA, 0.1 % Triton X-100, 0.1 mM DTNB, 0.5 mM Acrylyl-CoA, 10 units Crotonase
PH: 8
Temperature: 30
uniprot:Q5XIE6 [3-hydroxyisobutyryl-CoA hydrolase(Enzyme) wildtype]

H2O + (S)_3_Hydroxyisobutyryl_CoA -> (S)_3_Hydroxyisobutyrate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl, 1 mM EDTA, 0.1 % Triton X-100, 0.1 mM DTNB, 0.5 mM Methacrylyl-CoA, 10 units Crotonase
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:ARA_VALDEG-PWY L-valine degradation I
BioCyc:META_VALDEG-PWY L-valine degradation I
BioCyc:HUMAN_VALDEG-PWY valine degradation
BioCyc:MOUSE_VALDEG-PWY valine degradation I
BioCyc:MOUSE_PWY3DJ-86 valine degradation
PlantCyc:ARA_VALDEG-PWY L-valine degradation I
PlantCyc:PLANT_VALDEG-PWY L-valine degradation I
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-HSA-70895 Branched-chain amino acid catabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-70895 Branched-chain amino acid catabolism
Reactome:R-RNO-70895 Branched-chain amino acid catabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DRE-70895 Branched-chain amino acid catabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-70895 Branched-chain amino acid catabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-70895 Branched-chain amino acid catabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SPO-70895 Branched-chain amino acid catabolism
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120734 Malonic Aciduria
PathBank:SMP0120786 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000016 Propanoate Metabolism
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0087234 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000201 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0002361 Fatty Acid Metabolism
PathBank:SMP0002368 Fatty Acid Elongation In Mitochondria
PathBank:SMP0087341 Propanoate Metabolism
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120748 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0002460 Valine Degradation
PathBank:SMP0087248 Propanoate Metabolism
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000198 Malonic Aciduria
PathBank:SMP0000502 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation