EC: 2.7.1.60

N-acylmannosamine kinase (ATP:N-acyl-D-mannosamine 6-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype NanK]

ATP + D_Glucose -> ADP + D_Glucose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADP+, 1 mM DTT, 8.5 mM MgCl2, 7.5 units Glucose 6-phosphate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK L84P]

ATP + N_Acetyl_D_mannosamine -> ADP + N_Acetyl_D_mannosamine_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype NanK]

ATP + N_Acetyl_D_mannosamine -> N_Acetyl_D_mannosamine_6_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK V138M]

ATP + N_Acetyl_D_mannosamine -> ADP + N_Acetyl_D_mannosamine_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype NanK]

N_Acetyl_D_mannosamine + ATP -> N_Acetyl_D_mannosamine_6_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK L84P]

ATP + N_Acetyl_D_mannosamine -> N_Acetyl_D_mannosamine_6_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK L84P]

D_Mannose + ATP -> ADP + D_Mannose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype NanK]

ATP + D_Mannose -> D_Mannose_6_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype NanK]

ATP + 1,5_Anhydro_D_glucitol -> 1,5_Anhydro_D_glucitol_6_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK L84P]

ATP + 1,5_Anhydro_D_glucitol -> 1,5_Anhydro_D_glucitol_6_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK V138M]

ATP + D_Glucose -> ADP + D_Glucose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADP+, 1 mM DTT, 8.5 mM MgCl2, 7.5 units Glucose 6-phosphate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK L84P]

D_Glucose + ATP -> D_Glucose_6_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADP+, 1 mM DTT, 8.5 mM MgCl2, 7.5 units Glucose 6-phosphate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype NanK]

ATP + D_Glucose -> ADP + D_Glucose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADP+, 1 mM DTT, 8.5 mM MgCl2, 7.5 units Glucose 6-phosphate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK L84P]

D_Glucose + ATP -> ADP + D_Glucose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADP+, 1 mM DTT, 8.5 mM MgCl2, 7.5 units Glucose 6-phosphate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK V138M]

ATP + D_Glucose -> ADP + D_Glucose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADP+, 1 mM DTT, 8.5 mM MgCl2, 7.5 units Glucose 6-phosphate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK V138M]

ATP + N_Acetyl_D_mannosamine -> ADP + N_Acetyl_D_mannosamine_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADH, 1 mM DTT, 5 mM phosphoenolpyruvate, 6-26 mM MgCl2, 5 mM KCl, 15 units Pyruvate kinase, 25 units Lactate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) mutant NanK L84P/V138M]

D_Glucose + ATP -> ADP + D_Glucose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Tris, 0.5 mM NADP+, 1 mM DTT, 8.5 mM MgCl2, 7.5 units Glucose 6-phosphate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype]

N_Acetyl_D_mannosamine + ATP -> ADP + N_Acetyl_D_mannosamine_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype]

ATP + N_Acetyl_D_mannosamine -> N_Acetyl_D_mannosamine_6_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype]

ATP + D_Glucose -> ADP + D_Glucose_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P45425 [N-acylmannosamine kinase(Enzyme) wildtype]

D_Glucose + ATP -> D_Glucose_6_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:Q9QZ08 [N-acylmannosamine kinase(Enzyme) wildtype His-tagged]

N_Acetyl_D_mannosamine + ATP -> N_Acetyl_D_mannosamine_6_phosphate + ADP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 60 mM Tris-HCl, 20 mM MgCl2, 10 mM Phosphoenolpyruvate, 2.5 U Pyruvate kinase
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6138 CMP-N-acetylneuraminate biosynthesis I (eukaryotes)
BioCyc:HUMAN_PWY-6517 N-acetylglucosamine degradation II
BioCyc:META_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation I
BioCyc:META_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:META_PWY-6145 superpathway of CMP-sialic acids biosynthesis
BioCyc:ECO_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation I
BioCyc:MOUSE_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:MOUSE_PWY-6138 CMP-N-acetylneuraminate biosynthesis I (eukaryotes)
BioCyc:MOUSE_PWY-6517 N-acetylglucosamine degradation II
BioCyc:ECOL316407_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:META_PWY-6138 CMP-N-acetylneuraminate biosynthesis I (eukaryotes)
BioCyc:META_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:ECO_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:VCHO_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:ECOL316407_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation
BioCyc:VCHO_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation I
BioCyc:VCHO_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:MOUSE_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation
PathBank:SMP0002044 N-Acetylneuraminate, N-Acetylmannosamine, and N-Acetylglucosamine Degradation
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-4085001 Sialic acid metabolism
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-4085001 Sialic acid metabolism
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-4085001 Sialic acid metabolism
Reactome:R-RNO-446203 Asparagine N-linked glycosylation
Reactome:R-RNO-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-RNO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
PathBank:SMP0120666 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120714 Homocarnosinosis
PathBank:SMP0120818 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0120850 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120524 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000240 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0087169 Glutamate Metabolism
PathBank:SMP0087278 Glutamate Metabolism
PathBank:SMP0120737 Sialuria or French Type Sialuria
PathBank:SMP0120765 Tay-Sachs Disease
PathBank:SMP0000045 Amino Sugar Metabolism
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000216 Sialuria or French Type Sialuria
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0087281 Amino Sugar Metabolism
PathBank:SMP0120716 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120744 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120517 Sialuria or French Type Sialuria
PathBank:SMP0120545 Tay-Sachs Disease
PathBank:SMP0000390 Tay-Sachs Disease
PathBank:SMP0087175 Amino Sugar Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120659 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120599 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000534 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0063580 Amino Sugar Metabolism