EC: 2.7.1.3

ketohexokinase (ATP:D-fructose 1-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q02974 [ketohexokinase(Enzyme) wildtype]

D_Fructose + ATP -> D_Fructose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Triethanolamine, 100 mM KCl, 3.33 units/ml Pyruvate kinase, 3.33 units/ml Lactate dehydrogenase, 0.33 mM NADH, 3.33 mM Phosphoenolpyruvate, 1.33-20 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:Q02974 [ketohexokinase(Enzyme) wildtype]

D_Fructose + ATP -> D_Fructose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Triethanolamine, 100 mM KCl, 3.33 units/ml Pyruvate kinase, 3.33 units/ml Lactate dehydrogenase, 0.33 mM NADH, 3.33 mM Phosphoenolpyruvate, 20 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:Q02974 [ketohexokinase(Enzyme) wildtype]

ATP + L_Sorbose -> L_Sorbose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Triethanolamine, 100 mM KCl, 3.33 units/ml Pyruvate kinase, 3.33 units/ml Lactate dehydrogenase, 0.33 mM NADH, 3.33 mM Phosphoenolpyruvate, 20 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:Q02974 [K+]

ATP + D_Fructose -> D_Fructose_1_phosphate + ADP		 (( (Vmax * A) ) / (( (Km * (1 + (P / Ki))) ) + A)) buffer: 10 mM Tris/HCl, 6 mM MgCl2
PH: 7.4
Temperature: 36
uniprot:Q02974 [K+]

D_Fructose + ATP -> D_Fructose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris/HCl, 0-6 mM MgCl2
PH: 7.4
Temperature: 36
uniprot:Q02974 [K+]

GTP + D_Fructose -> GDP + D_Fructose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris/HCl, 0.5-6 mM MgCl2
PH: 7.4
Temperature: 36
uniprot:Q02974 [K+]

D_Fructose + GTP -> D_Fructose_1_phosphate + GDP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris/HCl, 6 mM MgCl2
PH: 7.4
Temperature: 36
uniprot:Q02974 [ketohexokinase(Enzyme) wildtype]

D_Xylulose + ATP -> ADP + D_Xylulose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Triethanolamine, 100 mM KCl, 3.33 units/ml Pyruvate kinase, 3.33 units/ml Lactate dehydrogenase, 0.33 mM NADH, 3.33 mM Phosphoenolpyruvate, 20 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:Q02974 [ketohexokinase(Enzyme) wildtype]

L_Galactoheptulose + ATP -> L_Galactoheptulose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Triethanolamine, 100 mM KCl, 3.33 units/ml Pyruvate kinase, 3.33 units/ml Lactate dehydrogenase, 0.33 mM NADH, 3.33 mM Phosphoenolpyruvate, 20 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:Q0II75 [K+]

D_Fructose + ATP -> D_Fructose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM phosphate, 10 units/ml ATP-creatine transphosphorylase
PH: 7.3
Temperature: 30
uniprot:Q0II75 [K+]

ATP + D_Fructose -> ADP + D_Fructose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Tris sulfate, 10 units/ml ATP-creatine transphosphorylase
PH: 7.3
Temperature: 30
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform A]

D_Fructose + ATP -> D_Fructose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform C]

D_Fructose + ATP -> D_Fructose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) mutant isoform A Ala43Thr]

ATP + D_Fructose -> D_Fructose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) mutant isoform C Ala43Thr]

ATP + D_Fructose -> ADP + D_Fructose_1_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform C]

ATP + D_Fructose -> D_Fructose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform A]

ATP + D_Fructose -> D_Fructose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) mutant isoform A Ala43Thr]

D_Fructose + ATP -> ADP + D_Fructose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) mutant isoform C Ala43Thr]

ATP + D_Fructose -> D_Fructose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform A]

ATP + D_Ribulose -> ADP + D_Ribulose_1_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform A]

L_Sorbose + ATP -> L_Sorbose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform A]

ATP + D_Ribose -> D_Ribose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform C]

ATP + D_Xylulose -> ADP + D_Xylulose_1_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform A]

D_Xylulose + ATP -> D_Xylulose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform C]

ATP + D_Ribulose -> ADP + D_Ribulose_1_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform C]

ATP + L_Sorbose -> ADP + L_Sorbose_1_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P50053 [ketohexokinase(Enzyme) wildtype isoform C]

D_Ribose + ATP -> D_Ribose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Na-PIPES, 6 mM MgCl2, 1 mM Phosphoenolpyruvate, 100 mM KCl, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 40 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY66-373 sucrose degradation V (sucrose α-glucosidase)
BioCyc:MOUSE_PWY66-373 sucrose degradation V (mammalian)
BioCyc:HUMAN_PWY66-373 sucrose degradation
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-RNO-70350 Fructose catabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-70350 Fructose catabolism
Reactome:R-MMU-70350 Fructose catabolism
Reactome:R-HSA-5652084 Fructose metabolism
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-5652084 Fructose metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-5663084 Diseases of carbohydrate metabolism
Reactome:R-HSA-5657562 Essential fructosuria
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-RNO-5652084 Fructose metabolism
PathBank:SMP0000064 Fructose and Mannose Degradation
PathBank:SMP0000725 Fructose Intolerance, Hereditary
PathBank:SMP0000561 Fructosuria