EC: 2.7.1.107

diacylglycerol kinase (ATP) (ATP:1,2-diacyl-sn-glycerol 3-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P20192 [3alpha,12alpha-Dihydroxy-5beta-cholanate]

ATP + sn_1,2_Dioleoylglycerol -> Dioleoyl_phosphatidic_acid + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 20 mM NaF, 0.5 mM Dithiothreitol
PH: 7.4
Temperature: 30
uniprot:P20192 [Dioleoylphosphatidylcholine]

ATP + sn_1,2_Dioleoylglycerol -> Dioleoyl_phosphatidic_acid + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 20 mM NaF, 0.5 mM Dithiothreitol
PH: 7.4
Temperature: 30
uniprot:Q9ESW4 [Mg2+]

ATP + sn_1,2_Dioleoylglycerol -> Dioleoyl_phosphatidic_acid + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM MOPS, 100 mM NaCl, 1 mM EGTA, 3 mM CaCl2
PH: 7.2
Temperature: 37
uniprot:P20192 [3alpha,12alpha-Dihydroxy-5beta-cholanate]

sn_1,2_Dioleoylglycerol + ATP -> ADP + Dioleoyl_phosphatidic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 20 mM NaF, 0.5 mM Dithiothreitol
PH: 7.4
Temperature: 30
uniprot:P20192 [Dioleoylphosphatidylcholine]

ATP + sn_1,2_Dioleoylglycerol -> Dioleoyl_phosphatidic_acid + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 20 mM NaF, 0.5 mM Dithiothreitol
PH: 7.4
Temperature: 30
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype]

sn_1,2_Dioleoylglycerol + ATP -> Dioleoyl_phosphatidic_acid + ADP		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 5-10 mM MgCl2, 10 mM NaF, 1 mM dithiothreitol, 0.3 mM Triton X-100
PH: 7
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype]

sn_1,2_Dioleoylglycerol + ATP -> ADP + Dioleoyl_phosphatidic_acid		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 5-10 mM MgCl2, 10 mM NaF, 1 mM dithiothreitol, 25 mM n-Octyl-beta-D-glucopyranoside
PH: 7
Temperature: 37
uniprot:P49619 [3alpha,12alpha-Dihydroxy-5beta-cholanate]

ATP + sn_1,2_Dioleoylglycerol -> ADP + Dioleoyl_phosphatidic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 20 mM NaF, 0.5 mM Dithiothreitol
PH: 7.4
Temperature: 30
uniprot:Q9Y6T7 [3alpha,12alpha-Dihydroxy-5beta-cholanate]

ATP + sn_1,2_Dioleoylglycerol -> Dioleoyl_phosphatidic_acid + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 20 mM NaF, 0.5 mM Dithiothreitol
PH: 7.4
Temperature: 30
uniprot:P23743 [3alpha,12alpha-Dihydroxy-5beta-cholanate]

sn_1,2_Dioleoylglycerol + ATP -> ADP + Dioleoyl_phosphatidic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 20 mM NaF, 0.5 mM Dithiothreitol
PH: 7.4
Temperature: 30
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

sn_1,2_Dioleoylglycerol + ATP -> Dioleoyl_phosphatidic_acid + ADP		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM NaF, 1 mM DTT, 50 mM n-Octyl beta-D-glucopyranoside (OG), 8.1 mol % Phosphatidylserine
PH: 7
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

ATP + sn_1,2_Dioleoylglycerol -> ADP + Dioleoyl_phosphatidic_acid		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM NaF, 1 mM DTT, 50 mM n-Octyl beta-D-glucopyranoside (OG), 8.1 mol % Phosphatidylserine
PH: 7
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

sn_1,2_Dioleoylglycerol + ATP -> ADP + Dioleoyl_phosphatidic_acid		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM NaF, 1 mM DTT, 50 mM n-Octyl beta-D-glucopyranoside (OG), 8.1 mol % Phosphatidylserine
PH: 7
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

sn_1,2_Dioctanoylglycerol + ATP -> ADP + Dioctanoyl_phosphatidic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM n-Octyl beta-D-glucopyranoside, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

sn_1,2_Dihexanoylglycerol + ATP -> ADP + Dihexanoyl_phosphatidic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM n-Octyl beta-D-glucopyranoside, 1 mM DTT
PH: 7
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

ATP + sn_1,2_Dioleoylglycerol -> ADP + Dioleoyl_phosphatidic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM NaF, 1 mM DTT, 50 mM n-Octyl beta-D-glucopyranoside (OG), 8.1 mol % Phosphatidylserine
PH: 7
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

sn_1,2_Dioleoylglycerol + ATP -> Dioleoyl_phosphatidic_acid + ADP		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM NaF, 1 mM DTT, 50 mM Triton X-100, 8.1 mol % Phosphatidylserine
PH: 7
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

sn_1,2_Dioleoylglycerol + ATP -> Dioleoyl_phosphatidic_acid + ADP		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM NaF, 1 mM DTT, 50 mM Triton X-100, 8.1 mol % Phosphatidylserine
PH: 7
Temperature: 37
uniprot:P34125 [diacylglycerol kinase(Enzyme) wildtype DGKA]

sn_1,2_Dioleoylglycerol + ATP -> Dioleoyl_phosphatidic_acid + ADP		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 10 mM NaF, 1 mM DTT, 50 mM n-Octyl beta-D-glucopyranoside (OG), 8.1 mol % Phosphatidylserine
PH: 7
Temperature: 37
uniprot:Q09103 [diacylglycerol kinase(Enzyme) wildtype]

1,2_Diacyl_sn_glycerol + ATP -> ADP + 1,2_Diacyl_sn_glycerol_3_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Phosphate, 100 mM KCl, 10 mM NaCl, 10 mM KF, 1 mM EGTA
PH: 6.8
Temperature: 22
uniprot:Q09103 [diacylglycerol kinase(Enzyme) mutant K014/+]

ATP + 1,2_Diacyl_sn_glycerol -> ADP + 1,2_Diacyl_sn_glycerol_3_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Phosphate, 100 mM KCl, 10 mM NaCl, 10 mM KF, 1 mM EGTA
PH: 6.8
Temperature: 22
uniprot:P0ABN1 [diacylglycerol kinase(Enzyme) wildtype]

N_Acylsphingosine + ATP -> Ceramide_1_phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 50 mM Tris-phosphate, 100 mM NaCl, 10 mM MgCl2, 0.7% (w/v) Triton X-100, 1 mM 2- mercaptoethanol, 0.4 mM dilauryl phosphatidylethanolamine, 0.4 mM bovine cardiolipin
PH: 6.6
Temperature: 37
uniprot:P0ABN1 [diacylglycerol kinase(Enzyme) wildtype]

1,2_Dipalmitoyl_glycerol + ATP -> Dipalmitoyl_phosphatidic_acid + ADP		 (( (V * A) ) / (Km + A)) buffer: 50 mM Tris-phosphate, 100 mM NaCl, 10 mM MgCl2, 0.7% (w/v) Triton X-100, 1 mM 2- mercaptoethanol, 0.4 mM dilauryl phosphatidylethanolamine, 0.4 mM bovine cardiolipin
PH: 6.6
Temperature: 37
uniprot:P0ABN1 [diacylglycerol kinase(Enzyme) wildtype]

1,2_Dipalmitoyl_glycerol + ATP -> Dipalmitoyl_phosphatidic_acid + ADP		 (( (V * A) ) / (Km + A)) buffer: 50 mM Tris-phosphate, 100 mM NaCl, 10 mM MgCl2, 0.7% (w/v) Triton X-100, 1 mM 2- mercaptoethanol, 0.4 mM dilauryl phosphatidylethanolamine, 0.4 mM bovine cardiolipin
PH: 6.6
Temperature: 37
- [Cardiolipin]

ATP + sn_1,2_Dioleoylglycerol -> Dioleoyl_phosphatidic_acid + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Bis-Tris, 0.1 mM EDTA, 1 mM spermine, 0.5 mM DTT, 1 mM sodium-deoxycholate, 0.02% Triton X-100
PH: 7.5
Temperature: 25
- [Cardiolipin]

ATP + sn_1,2_Dioleoylglycerol -> ADP + Dioleoyl_phosphatidic_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Bis-Tris, 0.1 mM EDTA, 1 mM spermine, 0.5 mM DTT, 1 mM sodium-deoxycholate, 0.02% Triton X-100
PH: 7.5
Temperature: 25
uniprot:Q9FFN7 [diacylglycerol kinase(Enzyme) wildtype AtDGK2 His6-tagged/NusA tagged]

ATP + sn_1,2_Dioleoylglycerol -> ADP + Dioleoyl_phosphatidic_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Bis-Tris, 5 mM MgCl2, 0.1 mM EDTA, 1 mM spermine, 0.5 mM dithiothreitol, 1 mM sodium deoxycholate, 0.02% Triton X-100, 250 µM cardiolipin
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:ARA_PWY-7039 phosphatidate metabolism, as a signaling molecule
BioCyc:META_PWY-7039 phosphatidate metabolism, as a signaling molecule
PlantCyc:ARA_PWY-7039 phosphatidate metabolism, as a signaling molecule
PlantCyc:PLANT_PWY-7039 phosphatidate metabolism, as a signaling molecule
Reactome:R-DDI-76002 Platelet activation, signaling and aggregation
Reactome:R-DDI-114508 Effects of PIP2 hydrolysis
Reactome:R-DDI-416476 G alpha (q) signalling events
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-6802957 Oncogenic MAPK signaling
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-DRE-1483206 Glycerophospholipid biosynthesis
Reactome:R-DDI-372790 Signaling by GPCR
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-556833 Metabolism of lipids
Reactome:R-DDI-109582 Hemostasis
Reactome:R-HSA-1483257 Phospholipid metabolism
Reactome:R-HSA-5663202 Diseases of signal transduction by growth factor receptors and second messengers
Reactome:R-HSA-6802952 Signaling by BRAF and RAF1 fusions
Reactome:R-MMU-1483257 Phospholipid metabolism
Reactome:R-DRE-1483257 Phospholipid metabolism
Reactome:R-DDI-162582 Signaling Pathways
Reactome:R-DDI-388396 GPCR downstream signalling
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1483206 Glycerophospholipid biosynthesis
Reactome:R-MMU-1483206 Glycerophospholipid biosynthesis
PathBank:SMP0120706 Glycerol Kinase Deficiency
PathBank:SMP0120814 Familial Lipoprotein Lipase Deficiency
PathBank:SMP0087322 Phospholipid Biosynthesis
PathBank:SMP0120813 D-Glyceric Acidura
PathBank:SMP0087228 Phospholipid Biosynthesis
PathBank:SMP0000025 Phospholipid Biosynthesis
PathBank:SMP0087324 Glycerolipid Metabolism
PathBank:SMP0087230 Glycerolipid Metabolism
PathBank:SMP0087447 Phospholipid Biosynthesis