EC: 2.5.1.55

3-deoxy-8-phosphooctulonate synthase (phosphoenolpyruvate:D-arabinose-5-phosphate C-(1-carboxyvinyl)transferase (phosphate-hydrolysing, 2-carboxy-2-oxoethyl-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q8GLK7 [Mn2+]

D_Arabinose_5_phosphate + H2O + Phosphoenolpyruvate -> Phosphate + 3_Deoxy_D_manno_octulosonate_8_phosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 60
uniprot:Q8GLK7 [Cd2+]

D_Arabinose_5_phosphate + Phosphoenolpyruvate + H2O -> Phosphate + 3_Deoxy_D_manno_octulosonate_8_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 60
uniprot:Q8GLK7 [Cd2+]

Phosphoenolpyruvate + H2O + D_Arabinose_5_phosphate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 60
uniprot:Q8GLK7 [Mn2+]

H2O + Phosphoenolpyruvate + D_Arabinose_5_phosphate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 60
uniprot:Q8GLK7 [3-deoxy-8-phosphooctulonate synthase(Enzyme) mutant KdsA C11N]

H2O + D_Arabinose_5_phosphate + Phosphoenolpyruvate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 60
uniprot:Q8GLK7 [3-deoxy-8-phosphooctulonate synthase(Enzyme) mutant KdsA C11N]

H2O + Phosphoenolpyruvate + D_Arabinose_5_phosphate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 60
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype KdsA]

Phosphoenolpyruvate + H2O + D_Arabinose_5_phosphate -> Phosphate + 3_Deoxy_D_manno_octulosonate_8_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype KdsA]

H2O + Phosphoenolpyruvate + D_Arabinose_5_phosphate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) mutant KdsA N26C]

D_Arabinose_5_phosphate + H2O + Phosphoenolpyruvate -> Phosphate + 3_Deoxy_D_manno_octulosonate_8_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) mutant KdsA N26C]

D_Arabinose_5_phosphate + Phosphoenolpyruvate + H2O -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) mutant KdsA N26C]

D_Arabinose_5_phosphate + Phosphoenolpyruvate + H2O -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) mutant KdsA N26C]

H2O + Phosphoenolpyruvate + D_Arabinose_5_phosphate -> Phosphate + 3_Deoxy_D_manno_octulosonate_8_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q8GLK7 [Mn2+]

Phosphoenolpyruvate + D_Arabinose_5_phosphate + H2O -> Phosphate + 3_Deoxy_D_manno_octulosonate_8_phosphate		 (( (( (E * kcat) ) * M) ) / (Km + M)) buffer: 100 mM HEPES
PH: 7.5
Temperature: 60
uniprot:Q8GLK7 [Cd2+]

D_Arabinose_5_phosphate + Phosphoenolpyruvate + H2O -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * M) ) / (Km + M)) buffer: 100 mM HEPES
PH: 7.5
Temperature: 60
uniprot:Q8GLK7 [Cd2+]

D_Arabinose_5_phosphate + Phosphoenolpyruvate + H2O -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * M) ) / (Km + M)) buffer: 100 mM HEPES
PH: 7.5
Temperature: 60
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) mutant KdsA N26C]

H2O + D_Arabinose_5_phosphate + Phosphoenolpyruvate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) mutant KdsA N26C]

H2O + Phosphoenolpyruvate + D_Arabinose_5_phosphate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype]

D_Arabinose_5_phosphate + H2O + Phosphoenolpyruvate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-acetate
PH: 7.6
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate + D_Erythrose_4_phosphate -> Phosphate + 2_Dehydro_3_deoxy_D_arabino_heptonate_7_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-acetate
PH: 7.6
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype]

Phosphoenolpyruvate + D_Ribose_5_phosphate + H2O -> 3_Deoxy_D_altro_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-acetate
PH: 7.6
Temperature: 37
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype]

2_Deoxy_D_ribose_5_phosphate + H2O + Phosphoenolpyruvate -> 3,5_Dideoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-acetate
PH: 7.6
Temperature: 37
uniprot:Q9ZN55 [Cd2+]

H2O + Phosphoenolpyruvate + D_Arabinose_5_phosphate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium maleate, 0.001% Brij 35
PH: 6.5
Temperature: 22
uniprot:Q9ZN55 [Cd2+]

D_Arabinose_5_phosphate + Phosphoenolpyruvate + H2O -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium maleate, 0.001% Brij 35
PH: 6.5
Temperature: 22
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype]

D_Arabinose_5_phosphate + Phosphoenolpyruvate + H2O -> Phosphate + 3_Deoxy_D_manno_octulosonate_8_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium maleate, 0.001% Brij 35
PH: 6.5
Temperature: 22
uniprot:P0A715 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate + D_Arabinose_5_phosphate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium maleate, 0.001% Brij 35
PH: 6.5
Temperature: 22
uniprot:Q9ZN55 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype]

D_Arabinose_5_phosphate + H2O + Phosphoenolpyruvate -> 3_Deoxy_D_manno_octulosonate_8_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium maleate, 0.001% Brij 35
PH: 6.5
Temperature: 22
uniprot:Q9ZN55 [3-deoxy-8-phosphooctulonate synthase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate + D_Arabinose_5_phosphate -> Phosphate + 3_Deoxy_D_manno_octulosonate_8_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium maleate, 0.001% Brij 35
PH: 6.5
Temperature: 22

Pathways

pathway id name
BioCyc:META_PWY-1269 CMP-3-deoxy-D-manno-octulosonate biosynthesis
BioCyc:SYNEL_PWY-1269 CMP-3-deoxy-D-manno-octulosonate biosynthesis
BioCyc:HPY_PWY-1269 CMP-3-deoxy-D-manno-octulosonate biosynthesis
BioCyc:ECOL316407_PWY-1269 CMP-KDO biosynthesis I
BioCyc:ECO_PWY-1269 CMP-3-deoxy-D-manno-octulosonate biosynthesis
BioCyc:ARA_PWY-1269 CMP-3-deoxy-D-manno-octulosonate biosynthesis
BioCyc:VCHO_PWY-1269 CMP-3-deoxy-D-manno-octulosonate biosynthesis
BioCyc:ECOO157_PWY-5111 CMP-KDO biosynthesis II (from D-arabinose 5-phosphate)
BioCyc:CAULO_PWY-1269 CMP-KDO biosynthesis I
BioCyc:CAULONA1000_PWY-5111 CMP-KDO biosynthesis II (from D-arabinose 5-phosphate)
BioCyc:ARA_PWY-5111 CMP-KDO biosynthesis II (from D-arabinose 5-phosphate)
BioCyc:ECOL199310_PWY-5111 CMP-KDO biosynthesis II (from D-arabinose 5-phosphate)
BioCyc:CAULO_PWY-5111 CMP-KDO biosynthesis II (from D-arabinose 5-phosphate)
BioCyc:CAULONA1000_PWY-1269 CMP-KDO biosynthesis I
PathBank:SMP0000851 Lipopolysaccharide Biosynthesis
PathBank:SMP0001919 Lipopolysaccharide Biosynthesis II
PathBank:SMP0002071 Lipopolysaccharide Biosynthesis III
PlantCyc:ARA_PWY-1269 CMP-3-deoxy-D-manno-octulosonate biosynthesis
PlantCyc:PLANT_PWY-5111 CMP-KDO biosynthesis II (from D-arabinose 5-phosphate)
PlantCyc:ARA_PWY-5111 CMP-KDO biosynthesis II (from D-arabinose 5-phosphate)
PlantCyc:PLANT_PWY-1269 CMP-3-deoxy-D-manno-octulosonate biosynthesis
PathBank:SMP0122112 Lipopolysaccharide Biosynthesis II
PathBank:SMP0012057 CMP-3-Deoxy-D-Manno-Octulosonate (CMP-Kdo) Biosynthesis
PathBank:SMP0121299 Lipopolysaccharide Biosynthesis
PathBank:SMP0122237 Lipopolysaccharide Biosynthesis III