EC: 2.4.2.28

S-methyl-5'-thioadenosine phosphorylase (S-methyl-5'-thioadenosine:phosphate S-methyl-5-thio-alpha-D-ribosyl-transferase)

enzyme lambda metabolic reaction experiment
uniprot:A0QR54 [S-methyl-5'-thioadenosine phosphorylase(Enzyme) wildtype MTAP]

5'_Deoxy_5'_(methylthio)adenosine + Phosphate -> Adenine + 5_Methylthio_D_ribose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.3
Temperature: 37
uniprot:A0QR54 [S-methyl-5'-thioadenosine phosphorylase(Enzyme) wildtype MTAP]

Adenosine + Phosphate -> Adenine + alpha_D_Ribose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.3
Temperature: 37
uniprot:Q8U4Q8 [S-methyl-5'-thioadenosine phosphorylase(Enzyme) wildtype MTAP1]

5'_Deoxy_5'_(methylthio)adenosine + Phosphate -> 5_Methylthio_D_ribose_1_phosphate + Adenine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 7.4
Temperature: 80
uniprot:Q8U4Q8 [S-methyl-5'-thioadenosine phosphorylase(Enzyme) wildtype MTAP1]

5'_Deoxy_5'_(methylthio)adenosine + Phosphate -> 5_Methylthio_D_ribose_1_phosphate + Adenine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 7.4
Temperature: 80
uniprot:Q8U4Q8 [S-methyl-5'-thioadenosine phosphorylase(Enzyme) wildtype MTAP1]

Phosphate + Inosine -> Hypoxanthine + alpha_D_Ribose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 7.4
Temperature: 80
- [S-methyl-5'-thioadenosine phosphorylase(Enzyme) wildtype]

Phosphate + 5'_Deoxy_5'_(methylthio)adenosine -> 5_Methylthio_D_ribose_1_phosphate + Adenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium Hepes, 5 mM NaH2PO4
PH: 7.5
Temperature: 37
- [S-methyl-5'-thioadenosine phosphorylase(Enzyme) wildtype]

Phosphate + 5'_Deoxy_5'_(methylthio)adenosine -> 5_Methylthio_D_ribose_1_phosphate + Adenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium Hepes, 5 mM NaH2PO4
PH: 7.5
Temperature: 37
- [S-methyl-5'-thioadenosine phosphorylase(Enzyme) wildtype]

Phosphate + 5'_Deoxy_5'_(methylthio)adenosine -> Adenine + 5_Methylthio_D_ribose_1_phosphate		 (( (( (V * A) ) * B) ) / (( (( (K_ia * K_b) ) + (K_b * A)) ) + (A * B))) buffer: 58 mM Tris-HCl
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6756 S-methyl-5'-thioadenosine degradation II
BioCyc:META_PWY-7527 L-methionine salvage cycle III
BioCyc:HUMAN_PWY-6756 S-methyl-5'-thioadenosine degradation
BioCyc:YEAST_PWY-6756 S-methyl-5'-thioadenosine degradation
BioCyc:HUMAN_PWY-7527 methionine salvage cycle III
BioCyc:META_PWY-5532 nucleoside and nucleotide degradation (archaea)
BioCyc:YEAST_PWY3O-64 methionine salvage pathway
BioCyc:MTBH37RV_PWY-6756 S-methyl-5'-thioadenosine degradation II
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1614635 Sulfur amino acid metabolism
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-CEL-1237112 Methionine salvage pathway
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1237112 Methionine salvage pathway
Reactome:R-HSA-449147 Signaling by Interleukins
Reactome:R-HSA-9020591 Interleukin-12 signaling
Reactome:R-MMU-1237112 Methionine salvage pathway
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-1614635 Sulfur amino acid metabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-BTA-1614635 Sulfur amino acid metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-1614635 Sulfur amino acid metabolism
Reactome:R-DME-1237112 Methionine salvage pathway
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-168256 Immune System
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1237112 Methionine salvage pathway
Reactome:R-SPO-1237112 Methionine salvage pathway
Reactome:R-BTA-1237112 Methionine salvage pathway
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DRE-1237112 Methionine salvage pathway
Reactome:R-DME-1614635 Sulfur amino acid metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1614635 Sulfur amino acid metabolism
Reactome:R-HSA-1280215 Cytokine Signaling in Immune system
Reactome:R-HSA-447115 Interleukin-12 family signaling
Reactome:R-MMU-1614635 Sulfur amino acid metabolism
Reactome:R-SPO-1614635 Sulfur amino acid metabolism
PathBank:SMP0000177 Cystathionine beta-Synthase Deficiency
PathBank:SMP0000340 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0000033 Methionine Metabolism
PathBank:SMP0000222 Glycine N-Methyltransferase Deficiency
PathBank:SMP0000341 Hypermethioninemia
PathBank:SMP0000221 Methionine Adenosyltransferase Deficiency
PathBank:SMP0087456 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0000214 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000570 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0002310 Methionine Metabolism and Salvage
PathBank:SMP0087403 Methionine Metabolism
PathBank:SMP0087483 Methionine Metabolism