EC: 2.3.1.199

very-long-chain 3-oxoacyl-CoA synthase (malonyl-CoA:very-long-chain acyl-CoA malonyltransferase (decarboxylating and thioester-hydrolysing))

enzyme lambda metabolic reaction experiment
uniprot:A1L3X0 [very-long-chain 3-oxoacyl-CoA synthase(Enzyme) wildtype His6-Myc-3xFLAG-tagged]

Malonyl_CoA + alpha_Linolenoyl_CoA -> Coenzyme_A + (11Z,14Z,17Z)_3_Oxoicosatrienoyl_CoA + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 5 μM rotenone, 1 mM NADPH, 20 μM BSA
PH: 6.5
Temperature: 37
uniprot:A1L3X0 [very-long-chain 3-oxoacyl-CoA synthase(Enzyme) wildtype His6-Myc-3xFLAG-tagged]

alpha_Linolenoyl_CoA + Malonyl_CoA -> CO2 + (11Z,14Z,17Z)_3_Oxoicosatrienoyl_CoA + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 5 μM rotenone, 1 mM NADPH, 20 μM BSA
PH: 6.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7592 arachidonate biosynthesis III (metazoa)
BioCyc:HUMAN_PWY-7606 docosahexaenoate biosynthesis III (mammals)
BioCyc:META_PWY-7592 arachidonate biosynthesis III (6-desaturase, mammals)
BioCyc:META_PWY-7726 (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase)
BioCyc:META_PWY-7727 docosahexaenoate biosynthesis IV (4-desaturase, mammals)
BioCyc:YEAST_PWY-7036 very long chain fatty acid biosynthesis
BioCyc:YEAST_PWY-5972 stearate biosynthesis I (animals and fungi)
BioCyc:HUMAN_PWY-7049 icosapentaenoate biosynthesis II (metazoa)
BioCyc:META_PWY-7728 (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoate biosynthesis II (4-desaturase)
BioCyc:HUMAN_PWY-5972 stearate biosynthesis
BioCyc:META_PWY-7036 very long chain fatty acid biosynthesis II
BioCyc:META_PWY-5080 very long chain fatty acid biosynthesis I
BioCyc:META_PWY-7724 icosapentaenoate biosynthesis III (8-desaturase, mammals)
BioCyc:META_PWY-7049 icosapentaenoate biosynthesis II (6-desaturase, mammals)
BioCyc:META_PWY-5972 stearate biosynthesis I (animals and fungi)
BioCyc:META_PWY-7606 docosahexaenoate biosynthesis III (6-desaturase, mammals)
BioCyc:ARA_PWY-7036 very long chain fatty acid biosynthesis II
BioCyc:ARA_PWY-5080 very long chain fatty acid biosynthesis I
PlantCyc:ARA_PWY-5080 very long chain fatty acid biosynthesis I
PlantCyc:ARA_PWY-7036 very long chain fatty acid biosynthesis II
PlantCyc:PLANT_PWY-5080 very long chain fatty acid biosynthesis I
PlantCyc:PLANT_PWY-7036 very long chain fatty acid biosynthesis II
Reactome:R-HSA-9843745 Adipogenesis
Reactome:R-HSA-9843743 Transcriptional regulation of brown and beige adipocyte differentiation
Reactome:R-HSA-9844594 Transcriptional regulation of brown and beige adipocyte differentiation by EBF2
Reactome:R-HSA-9818564 Epigenetic regulation of gene expression by MLL3 and MLL4 complexes
Reactome:R-HSA-9917777 Epigenetic regulation by WDR5-containing histone modifying complexes
Reactome:R-HSA-9851695 Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes
Reactome:R-HSA-9841922 MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-75105 Fatty acyl-CoA biosynthesis
Reactome:R-CEL-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-HSA-75105 Fatty acyl-CoA biosynthesis
Reactome:R-HSA-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-75105 Fatty acyl-CoA biosynthesis
Reactome:R-MMU-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-BTA-8978868 Fatty acid metabolism
Reactome:R-CEL-556833 Metabolism of lipids
Reactome:R-CEL-75876 Synthesis of very long-chain fatty acyl-CoAs
Reactome:R-CEL-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-75876 Synthesis of very long-chain fatty acyl-CoAs
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
Reactome:R-HSA-212165 Epigenetic regulation of gene expression
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-BTA-75876 Synthesis of very long-chain fatty acyl-CoAs
Reactome:R-CEL-8978868 Fatty acid metabolism
Reactome:R-CEL-2046105 Linoleic acid (LA) metabolism
Reactome:R-DME-8978868 Fatty acid metabolism
Reactome:R-HSA-75876 Synthesis of very long-chain fatty acyl-CoAs
Reactome:R-HSA-2046105 Linoleic acid (LA) metabolism
Reactome:R-MMU-75876 Synthesis of very long-chain fatty acyl-CoAs
Reactome:R-MMU-2046105 Linoleic acid (LA) metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-75105 Fatty acyl-CoA biosynthesis
Reactome:R-RNO-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-75105 Fatty acyl-CoA biosynthesis
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-75105 Fatty acyl-CoA biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome:R-RNO-75876 Synthesis of very long-chain fatty acyl-CoAs
Reactome:R-RNO-2046105 Linoleic acid (LA) metabolism
PathBank:SMP0002324 Biosynthesis of Unsaturated Fatty Acids
PathBank:SMP0002346 Biosynthesis of Unsaturated Fatty Acids (Icosanoyl-CoA)
PathBank:SMP0002329 Biosynthesis of Unsaturated Fatty Acids (Docosanoyl-CoA)
PathBank:SMP0000018 Alpha Linolenic Acid and Linoleic Acid Metabolism
PathBank:SMP0002325 Biosynthesis of Unsaturated Fatty Acids (Tetracosanoyl-CoA)
PathBank:SMP0002347 Biosynthesis of Unsaturated Fatty Acids (Stearoyl-CoA)
PathBank:SMP0087171 Alpha Linolenic Acid and Linoleic Acid Metabolism
PathBank:SMP0087279 Alpha Linolenic Acid and Linoleic Acid Metabolism
PathBank:SMP0063593 Alpha Linolenic Acid and Linoleic Acid Metabolism