EC: 2.3.1.191

UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase ((3R)-3-hydroxyacyl-[acyl-carrier protein]:UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine N-acyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) wildtype N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant Y47A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant H276A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant D232A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant Q236A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant F41A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant N233A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant H239A N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant K46A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) wildtype]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (Vmax * A) ) * B) ) / (( (( (( (Kma * B) ) + (Kmb * A)) ) + (Kia * Kmb)) ) + (A * B))) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-2-N-[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Ki))))) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) wildtype]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Ki))))) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) wildtype]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant N233A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant D232A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant Q236A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant K46A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant K194A N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant R293A N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant H276A N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant Y47A N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant H239A N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant R293A N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant K194A N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [(R)-3-Hydroxylauroyl-methylphosphopantetheine]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (Vmax * S) ) / (Km + (S * (1 + (I / Ki))))) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [(R)-3-Hydroxylauroyl-methylphosphopantetheine]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [(R)-3-Hydroxylauroyl-methylphosphopantetheine]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [(R)-3-Hydroxylauroyl-methylphosphopantetheine]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + Holo_[acyl_carrier_protein]
(( (Vmax * S) ) / (Km + (S * (1 + (I / Ki))))) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-2-N-[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Ki))))) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) wildtype N-terminal His6-tag]

UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine + (3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30
uniprot:P21645 [UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase(Enzyme) mutant F41A N-terminal His6-tag]

(3R)_3_Hydroxytetradecanoyl_[acyl_carrier_protein] + UDP_3_O_(3_hydroxytetradecanoyl)_D_glucosamine -> Holo_[acyl_carrier_protein] + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Hepes, 0.02 mg/ml BSA
PH: 7.5
Temperature: 30

Pathways

pathway id name
PathBank:SMP0000851 Lipopolysaccharide Biosynthesis
PathBank:SMP0001919 Lipopolysaccharide Biosynthesis II
PathBank:SMP0002071 Lipopolysaccharide Biosynthesis III
PathBank:SMP0122112 Lipopolysaccharide Biosynthesis II
PathBank:SMP0121299 Lipopolysaccharide Biosynthesis
PathBank:SMP0122237 Lipopolysaccharide Biosynthesis III