EC: 2.1.2.2

phosphoribosylglycinamide formyltransferase 1 (10-formyltetrahydrofolate:5'-phosphoribosylglycinamide N-formyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q64737 [phosphoribosylglycinamide formyltransferase(Enzyme) wildtype]

5'_Phosphoribosylglycinamide + 10_Formyl_5,8_dideazafolate -> 5'_Phosphoribosyl_N_formylglycinamide + 5,8_Dideazafolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM HEPES, 50 µg/ml bovine serum albumin
PH: 7.5
Temperature: 25
uniprot:Q64737 [phosphoribosylglycinamide formyltransferase(Enzyme) wildtype]

10_Formyl_5,8_dideazafolate_hexaglutamate + 5'_Phosphoribosylglycinamide -> 5,8_Dideazafolate_hexaglutamate + 5'_Phosphoribosyl_N_formylglycinamide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM HEPES, 50 µg/ml bovine serum albumin
PH: 7.5
Temperature: 25
uniprot:Q64737 [phosphoribosylglycinamide formyltransferase(Enzyme) wildtype]

5'_Phosphoribosylglycinamide + 10_Formyl_5,8_dideazafolate -> 5,8_Dideazafolate + 5'_Phosphoribosyl_N_formylglycinamide		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 75 mM HEPES, 50 µg/ml bovine serum albumin
PH: 7.5
Temperature: 25
uniprot:Q64737 [phosphoribosylglycinamide formyltransferase(Enzyme) wildtype]

5'_Phosphoribosylglycinamide + 10_Formyl_5,8_dideazafolate -> 5'_Phosphoribosyl_N_formylglycinamide + 5,8_Dideazafolate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 75 mM HEPES, 50 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:Q64737 [phosphoribosylglycinamide formyltransferase(Enzyme) wildtype]

5'_Phosphoribosylglycinamide + 10_Formyl_5,8_dideazafolate -> 5'_Phosphoribosyl_N_formylglycinamide + 5,8_Dideazafolate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 75 mM HEPES, 50 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:Q64737 [phosphoribosylglycinamide formyltransferase(Enzyme) wildtype]

10_Formyl_5,8_dideazafolate + 5'_Phosphoribosylglycinamide -> 5'_Phosphoribosyl_N_formylglycinamide + 5,8_Dideazafolate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 75 mM HEPES, 50 µg/ml bovine serum albumin
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:META_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:YEAST_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:YEAST_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ARA_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBCDC1551_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:MOUSE_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:BSUB_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:HUMAN_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:META_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:YEAST_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:YEAST_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:ARA_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:MTBH37RV_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:MTBH37RV_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:MOUSE_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ECOL316407_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ECO_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECO_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ARA_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:MTBH37RV_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:MOUSE_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:MOUSE_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:BSUB_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:BSUB_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:ECOL316407_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:HUMAN_PWY-841 purine nucleotides de novo biosynthesis
BioCyc:META_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:META_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:META_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:MTBH37RV_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:MTBCDC1551_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:MTBCDC1551_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:MTBCDC1551_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:BSUB_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:BSUB_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
PathBank:SMP0002042 Thiamin Diphosphate Biosynthesis
PlantCyc:PLANT_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
PlantCyc:PLANT_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
PlantCyc:ARA_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
PlantCyc:ARA_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
PlantCyc:ARA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
Reactome:R-BTA-1430728 Metabolism
Reactome:R-GGA-1660598 Metabolism
Reactome:R-HSA-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-BTA-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-GGA-419470 Nucleotide metabolism
Reactome:R-GGA-419140 De novo synthesis of IMP
Reactome:R-HSA-8956320 Nucleotide biosynthesis
Reactome:R-MMU-8956320 Nucleotide biosynthesis
Reactome:R-BTA-15869 Nucleotide metabolism
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-BTA-8956320 Nucleotide biosynthesis
Reactome:R-DME-8956320 Nucleotide biosynthesis
Reactome:R-GGA-419474 Purine metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0002334 Tetrahydrofolate Biosynthesis
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0087239 Purine Metabolism