uniprot:P0DI90 [FAD]
O2 + H2O + L_Phenylalanine -> NH3 + H2O2 + Phenylpyruvate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Kis))))) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P86535 [FAD]
H2O + O2 + L_Phenylalanine -> NH3 + Phenylpyruvate + H2O2 |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Kis))))) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P86535 [FAD]
L_Phenylalanine + O2 + H2O -> Phenylpyruvate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P0DI90 [FAD]
L_Phenylalanine + O2 + H2O -> Phenylpyruvate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:G8XQX1 [FAD]
O2 + L_Lysine + H2O -> H2O2 + 6_Amino_2_oxohexanoate + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
O2 + H2O + L_Arginine -> H2O2 + NH3 + 5_Guanidino_2_oxopentanoate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
H2O + L_Arginine + O2 -> 5_Guanidino_2_oxopentanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
H2O + O2 + L_Valine -> alpha_Ketovaline + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
O2 + L_Alanine + H2O -> NH3 + H2O2 + Pyruvate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
L_Tyrosine + H2O + O2 -> H2O2 + NH3 + 3_(4_Hydroxyphenyl)pyruvate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:P0C2D7 [FAD]
H2O + O2 + L_Phenylalanine -> H2O2 + Phenylpyruvate + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 20 µg/ml horseradish peroxidase, 65 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
H2O + L_Isoleucine + O2 -> 3_Methyl_2_oxopentanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
L_Phenylalanine + O2 + H2O -> H2O2 + NH3 + Phenylpyruvate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:P0C2D7 [FAD]
L_Leucine + H2O + O2 -> NH3 + H2O2 + 4_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 20 µg/ml horseradish peroxidase, 65 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
L_Leucine + H2O + O2 -> H2O2 + 4_Methyl_2_oxopentanoate + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:P0C2D7 [FAD]
O2 + H2O + L_Methionine -> 4_Methylthio_2_oxobutanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 20 µg/ml horseradish peroxidase, 65 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
H2O + O2 + L_Tryptophan -> NH3 + Indolepyruvate + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:G8XQX1 [FAD]
L_Methionine + H2O + O2 -> 4_Methylthio_2_oxobutanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 150 mM NaCl, 400 mU/ml Horseradish peroxidase, 10 µM o-Dianisidine
PH: 7.5
Temperature: 25 |
uniprot:K9N7B7 [FAD]
O2 + H2O + L_Leucine -> 4_Methyl_2_oxopentanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 2 mM o-Phenylenediamine, 0.8 U/ml Horseradish peroxidase
PH: 8
Temperature: 37 |
uniprot:P0DI91 [FAD]
L_Phenylalanine + H2O + O2 -> NH3 + H2O2 + Phenylpyruvate |
(( (( (E * kcat) ) * Km) ) / (S + Km)) |
|
buffer: 0.1 M Tris-HCl, 0.26 mM o-Dianisidine, 7 U/ml Horseradish peroxidase
PH: 8.5
Temperature: 36 |
uniprot:P0DI91 [FAD]
L_Methionine + H2O + O2 -> H2O2 + NH3 + 4_Methylthio_2_oxobutanoate |
(( (( (E * kcat) ) * Km) ) / (S + Km)) |
|
buffer: 0.1 M Tris-HCl, 0.26 mM o-Dianisidine, 7 U/ml Horseradish peroxidase
PH: 8.5
Temperature: 36 |
uniprot:P0DI90 [Anthranilate]
O2 + H2O + L_Phenylalanine -> NH3 + H2O2 + Phenylpyruvate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Kis))))) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P0DI90 [FAD]
H2O + L_Phenylalanine + O2 -> Phenylpyruvate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Kis))))) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P86535 [FAD]
O2 + L_Phenylalanine + H2O -> H2O2 + NH3 + Phenylpyruvate |
(( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / Kis))))) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P0DI90 [FAD]
O2 + H2O + L_Isoleucine -> 3_Methyl_2_oxopentanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:A0A024BTN9 [FAD]
O2 + L_Leucine + H2O -> H2O2 + NH3 + 4_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 2 mM o-Phenylenediamine, 0.8 U/ml Horseradish peroxidase
PH: 8
Temperature: 37 |
uniprot:A8QL52 [FAD]
L_Tyrosine + H2O + O2 -> NH3 + 3_(4_Hydroxyphenyl)pyruvate + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
H2O + L_Phenylalanine + O2 -> H2O2 + NH3 + Phenylpyruvate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
L_Aspartate + O2 + H2O -> NH3 + Oxaloacetate + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
L_Tryptophan + H2O + O2 -> NH3 + Indolepyruvate + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
H2O + L_Glutamate + O2 -> 2_Oxoglutarate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
H2O + O2 + L_Glutamine -> NH3 + 2_Oxoglutaramate + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
L_Histidine + O2 + H2O -> 2_Oxo_3_[4_imidazolyl]_propanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:P86535 [FAD]
H2O + O2 + L_Tyrosine -> NH3 + H2O2 + 3_(4_Hydroxyphenyl)pyruvate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P0DI90 [FAD]
H2O + O2 + L_Tyrosine -> NH3 + H2O2 + 3_(4_Hydroxyphenyl)pyruvate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P86535 [FAD]
O2 + L_Isoleucine + H2O -> H2O2 + NH3 + 3_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P86535 [FAD]
H2O + L_Leucine + O2 -> H2O2 + 4_Methyl_2_oxopentanoate + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P0DI90 [FAD]
H2O + L_Leucine + O2 -> H2O2 + 4_Methyl_2_oxopentanoate + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P86535 [FAD]
O2 + H2O + L_Tryptophan -> NH3 + H2O2 + Indolepyruvate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P0DI90 [FAD]
H2O + L_Tryptophan + O2 -> Indolepyruvate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P86535 [FAD]
O2 + L_Methionine + H2O -> NH3 + H2O2 + 4_Methylthio_2_oxobutanoate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:P0DI90 [FAD]
O2 + L_Methionine + H2O -> 4_Methylthio_2_oxobutanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.05 M potassium phosphate, 350 mU/ml horseradish peroxidase, 10 µM O-dianisidine
PH: 6.8
Temperature: 25 |
uniprot:A8QL52 [FAD]
L_Isoleucine + H2O + O2 -> 3_Methyl_2_oxopentanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
H2O + L_Lysine + O2 -> H2O2 + 6_Amino_2_oxohexanoate + NH3 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
H2O + L_Arginine + O2 -> 5_Guanidino_2_oxopentanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
O2 + L_Leucine + H2O -> NH3 + H2O2 + 4_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
H2O + L_Methionine + O2 -> 4_Methylthio_2_oxobutanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:P0DI91 [FAD]
O2 + L_Leucine + H2O -> H2O2 + NH3 + 4_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * Km) ) / (S + Km)) |
|
buffer: 0.1 M Tris-HCl, 0.26 mM o-Dianisidine, 7 U/ml Horseradish peroxidase
PH: 8.5
Temperature: 36 |
uniprot:P0DI91 [FAD]
L_Tryptophan + H2O + O2 -> Indolepyruvate + NH3 + H2O2 |
(( (( (E * kcat) ) * Km) ) / (S + Km)) |
|
buffer: 0.1 M Tris-HCl, 0.26 mM o-Dianisidine, 7 U/ml Horseradish peroxidase
PH: 8.5
Temperature: 36 |
uniprot:A8QL52 [FAD]
H2O + L_Alanine + O2 -> NH3 + Pyruvate + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:A8QL52 [FAD]
L_Asparagine + H2O + O2 -> NH3 + 2_Oxosuccinamate + H2O2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris, 10 mM o-Dianisidine, 5 µU/ml Horseradish peroxidase
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Isoleucine + H2O -> NH3 + 3_Methyl_2_oxopentanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
L_Lysine + H2O + O2 -> NH3 + 6_Amino_2_oxohexanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Ornithine + O2 -> NH3 + H2O2 + 5_Amino_2_oxopentanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
L_Alanine + O2 + H2O -> NH3 + Pyruvate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_alpha_Aminobutyrate + O2 -> NH3 + 2_Oxobutyrate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + L_Glutamine -> NH3 + 2_Oxoglutaramate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Norvaline -> H2O2 + NH3 + 2_Oxopentanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + L_Asparagine -> H2O2 + 2_Oxosuccinamate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Histidine -> NH3 + 2_Oxo_3_[4_imidazolyl]_propanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Tyrosine + H2O -> H2O2 + NH3 + 3_(4_Hydroxyphenyl)pyruvate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
L_Phenylalanine + H2O + O2 -> NH3 + H2O2 + Phenylpyruvate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
L_Arginine + O2 + H2O -> NH3 + 5_Guanidino_2_oxopentanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + L_Tryptophan -> NH3 + H2O2 + Indolepyruvate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Leucine + H2O -> H2O2 + 4_Methyl_2_oxopentanoate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
L_Methionine + O2 + H2O -> NH3 + H2O2 + 4_Methylthio_2_oxobutanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
L_Valine + H2O + O2 -> alpha_Ketovaline + H2O2 + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Norleucine -> NH3 + H2O2 + 2_Oxohexanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0C2D4 [L-amino-acid oxidase(Enzyme) wildtype]
2 L_Cysteine + H2O + O2 -> H2O2 + NH3 + 2 2_Oxo_3_thio_propanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 0.0075% peroxidase, 67.5 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
- [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Leucine + H2O -> 4_Methyl_2_oxopentanoate + NH3 + H2O2 |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 9
Temperature: 37 |
- [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Methionine -> 4_Methylthio_2_oxobutanoate + NH3 + H2O2 |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 9
Temperature: 37 |
- [L-amino-acid oxidase(Enzyme) wildtype]
S_Adenosyl_L_homocysteine + H2O + O2 -> H2O2 + NH3 + 4_Thioadenosyl_2_oxobutyrate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 9
Temperature: 37 |
- [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + S_Adenosyl_L_homocysteine -> 4_Thioadenosyl_2_oxobutyrate + H2O2 + NH3 |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 30 U/ml catalase
PH: 9
Temperature: 37 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Lysine + H2O -> NH3 + 6_Amino_2_oxohexanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium phosphate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 7.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Lysine -> 6_Amino_2_oxohexanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium phosphate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 7
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Lysine + O2 + H2O -> NH3 + H2O2 + 6_Amino_2_oxohexanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium phosphate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 6.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Lysine + O2 -> NH3 + 6_Amino_2_oxohexanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium acetate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 6
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Leucine + O2 + H2O -> 4_Methyl_2_oxopentanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium phosphate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 7
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Leucine + H2O + O2 -> NH3 + H2O2 + 4_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium phosphate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 6.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Leucine + H2O -> H2O2 + 4_Methyl_2_oxopentanoate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Leucine + O2 -> 4_Methyl_2_oxopentanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium phosphate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 7.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Leucine + H2O + O2 -> NH3 + 4_Methyl_2_oxopentanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium acetate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 6
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Isoleucine + O2 -> NH3 + H2O2 + 3_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
L_Arginine + H2O + O2 -> H2O2 + 5_Guanidino_2_oxopentanoate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
L_Tryptophan + H2O + O2 -> NH3 + H2O2 + Indolepyruvate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Leucine + H2O -> NH3 + H2O2 + 4_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
L_Methionine + O2 + H2O -> H2O2 + NH3 + 4_Methylthio_2_oxobutanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_alpha_Aminobutyrate -> H2O2 + 2_Oxobutyrate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + L_Norvaline -> H2O2 + NH3 + 2_Oxopentanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
L_Norleucine + H2O + O2 -> NH3 + H2O2 + 2_Oxohexanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Histidine + H2O -> NH3 + H2O2 + 2_Oxo_3_[4_imidazolyl]_propanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Lysine + O2 -> 6_Amino_2_oxohexanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Ornithine + H2O -> H2O2 + 5_Amino_2_oxopentanoate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
L_Alanine + H2O + O2 -> H2O2 + Pyruvate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Glutamine + O2 -> H2O2 + 2_Oxoglutaramate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
L_Asparagine + H2O + O2 -> NH3 + 2_Oxosuccinamate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:Q07523 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Lysine + H2O -> NH3 + H2O2 + 6_Amino_2_oxohexanoate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 1 mM EDTA, 0.002 % 2,6-Dichloroindophenol
PH: 8.4
Temperature: 25 |
uniprot:Q07523 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + L_Tryptophan -> Indolepyruvate + NH3 + H2O2 |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 1 mM EDTA, 0.002 % 2,6-Dichloroindophenol
PH: 8.4
Temperature: 25 |
uniprot:Q07523 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Leucine + H2O -> H2O2 + 4_Methyl_2_oxopentanoate + NH3 |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 1 mM EDTA, 0.002 % 2,6-Dichloroindophenol
PH: 8.4
Temperature: 25 |
uniprot:Q07523 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Methionine + O2 -> NH3 + H2O2 + 4_Methylthio_2_oxobutanoate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 1 mM EDTA, 0.002 % 2,6-Dichloroindophenol
PH: 8.4
Temperature: 25 |
uniprot:P81382 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Phenylalanine + H2O -> NH3 + Phenylpyruvate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 67.5 µg/ml O-dianisidine, 0.0075% horseradish peroxidase (100 purpurogalin units/mg)
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Leucine + H2O + O2 -> 4_Methyl_2_oxopentanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M sodium acetate, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + Glycine -> Glyoxylate + NH3 + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Lysine -> NH3 + H2O2 + 6_Amino_2_oxohexanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Lysine + O2 + H2O -> 6_Amino_2_oxohexanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Lysine + O2 -> NH3 + 6_Amino_2_oxohexanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 10
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Lysine + H2O + O2 -> H2O2 + 6_Amino_2_oxohexanoate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 9
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Leucine + H2O -> NH3 + 4_Methyl_2_oxopentanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 10
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Glutamate -> H2O2 + 2_Oxoglutarate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Valine -> H2O2 + alpha_Ketovaline + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Serine + H2O + O2 -> NH3 + 3_Hydroxypyruvate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Alanine + H2O -> H2O2 + NH3 + Pyruvate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Glutamine + H2O + O2 -> NH3 + H2O2 + 2_Oxoglutaramate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Proline + O2 -> NH3 + 2_Oxopentanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_2,4_Diaminobutanoate + H2O -> NH3 + 2_Oxo_4_aminobutyrate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Asparagine + O2 -> H2O2 + NH3 + 2_Oxosuccinamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + 3,4_Dehydro_L_proline + H2O -> H2O2 + NH3 + 2_Oxopent_3_enoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + N_Acetyl_L_leucine -> NH3 + N_Acetyl_4_methyl_2_oxopentanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Tyrosine -> NH3 + H2O2 + 3_(4_Hydroxyphenyl)pyruvate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + L_Isoleucine -> NH3 + 3_Methyl_2_oxopentanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Phenylalanine + H2O + O2 -> Phenylpyruvate + H2O2 + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Lysine + H2O + O2 -> H2O2 + NH3 + 6_Amino_2_oxohexanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Arginine + H2O -> NH3 + 5_Guanidino_2_oxopentanoate + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Tryptophan -> Indolepyruvate + H2O2 + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Leucine -> 4_Methyl_2_oxopentanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Ornithine + O2 + H2O -> H2O2 + NH3 + 5_Amino_2_oxopentanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Leucine + H2O + O2 -> 4_Methyl_2_oxopentanoate + H2O2 + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Leucine + H2O + O2 -> H2O2 + NH3 + 4_Methyl_2_oxopentanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 9
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + H2O + L_Methionine -> NH3 + H2O2 + 4_Methylthio_2_oxobutanoate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_alpha_Aminobutyrate + H2O -> 2_Oxobutyrate + H2O2 + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
L_Norvaline + O2 + H2O -> H2O2 + 2_Oxopentanoate + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + L_Norleucine + O2 -> 2_Oxohexanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Histidine -> 2_Oxo_3_[4_imidazolyl]_propanoate + NH3 + H2O2 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Cystine + H2O -> NH3 + H2O2 + 2_Oxo_2_amino_beta_dithiolactic_acid |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
H2O + O2 + L_Leucine_methyl_ester -> NH3 + H2O2 + 4_Methyl_2_oxopentanoate_methyl_ester |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P81383 [L-amino-acid oxidase(Enzyme) wildtype]
O2 + L_Glutamic_acid_gamma_ethyl_ester + H2O -> H2O2 + 2_Oxoglutarate_gamma_ethyl_ester + NH3 |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris-HCl, 12.5 µg/ml horseradish peroxidase (100 purpurogalin U/mg), 20 µg/ml O-dianisidine
PH: 8.5
Temperature: 25 |
uniprot:P0DPS2 [FAD]
L_Leucine + H2O + O2 -> 4_Methyl_2_oxopentanoate + NH3 + H2O2 |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl, 2 mM o-Phenyldiamine, 1 mg/ml horseradish peroxidase
PH: 7.8
Temperature: 37 |
uniprot:P0DQH9 [L-amino-acid oxidase(Enzyme) wildtype isoform LAAOI]
L_Leucine + O2 + H2O -> H2O2 + NH3 + 4_Methyl_2_oxopentanoate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.81 U/ml horseradish peroxidase, 2 mM o-phenylenediamine
PH: 8
Temperature: 37 |
uniprot:P0DQH9 [L-amino-acid oxidase(Enzyme) wildtype isoform LAAOII]
H2O + O2 + L_Leucine -> NH3 + H2O2 + 4_Methyl_2_oxopentanoate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.81 U/ml horseradish peroxidase, 2 mM o-phenylenediamine
PH: 8
Temperature: 37 |