EC: 1.21.99.4

thyroxine 5'-deiodinase (3,3',5-triiodo-L-thyronine,iodide:acceptor oxidoreductase (iodinating))

enzyme lambda metabolic reaction experiment
uniprot:Q9IAX2 [thyroxine 5'-deiodinase(Enzyme) wildtype]

L_Thyroxine + Reduced_acceptor -> Triiodothyronine + Acceptor + I_ + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM EDTA, 20 mM DTT, 1 mM 6-n-propylthiouracil
PH: 6.9
Temperature: 37
uniprot:Q9IAX2 [thyroxine 5'-deiodinase(Enzyme) wildtype]

Reduced_acceptor + L_Thyroxine -> Triiodothyronine + Acceptor + H+ + I_		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM EDTA, 20 mM DTT, 1 mM 6-n-propylthiouracil
PH: 6.9
Temperature: 37
uniprot:Q9IAX2 [thyroxine 5'-deiodinase(Enzyme) wildtype]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> Acceptor + I_ + H+ + 3,3'_Diiodothyronine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM EDTA, 20 mM DTT, 1 mM 6-n-propylthiouracil
PH: 6.9
Temperature: 37
uniprot:Q9Z1Y9 [thyroxine 5'-deiodinase(Enzyme) wildtype]

Reduced_acceptor + L_Thyroxine -> Acceptor + H+ + Triiodothyronine + I_		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Phosphate, 1 mM EDTA, 25 mM DTT
PH: 7
Temperature: 37
uniprot:Q9Z1Y9 [thyroxine 5'-deiodinase(Enzyme) wildtype]

3,3',5'_Triiodothyronine + Reduced_acceptor -> Acceptor + 3,3'_Diiodothyronine + H+ + I_		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Phosphate, 1 mM EDTA, 25 mM DTT
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> 3,3'_Diiodothyronine + H+ + Acceptor + I_		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 1 mM EDTA
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> I_ + Acceptor + 3,3'_Diiodothyronine + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris/HCl, 0.25 M sucrose, 1 mM EDTA, 42 nM thioredoxin reductase, 0.5 mM NADPH
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> I_ + Acceptor + H+ + 3,3'_Diiodothyronine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris/HCl, 0.25 M sucrose, 1 mM EDTA
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> 3,3'_Diiodothyronine + H+ + Acceptor + I_		 (( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris/HCl, 0.25 M sucrose, 1 mM EDTA
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> 3,3'_Diiodothyronine + Acceptor + I_ + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris/HCl, 0.25 M sucrose, 1 mM EDTA
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> I_ + 3,3'_Diiodothyronine + H+ + Acceptor		 (( (Vmax * A) ) / (Km + A)) buffer: 0.01 M sodium phosphate, 0.15 M NaCl
PH: 7.4
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> I_ + 3,3'_Diiodothyronine + H+ + Acceptor		 (( (Vmax * A) ) / (Km + A)) buffer: 0.01 M sodium phosphate, 0.15 M NaCl
PH: 7.4
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> Acceptor + H+ + 3,3'_Diiodothyronine + I_		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 1 mM EDTA
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> 3,3'_Diiodothyronine + Acceptor + H+ + I_		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 1 mM EDTA, 42 nM thioredoxin reductase, 0.5 mM NADPH
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> Acceptor + H+ + I_ + 3,3'_Diiodothyronine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris/HCl, 0.25 M sucrose, 1 mM EDTA, 42 nM thioredoxin reductase, 0.5 mM NADPH
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> 3,3'_Diiodothyronine + Acceptor + I_ + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 1 mM EDTA
PH: 7
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> H+ + Acceptor + I_ + 3,3'_Diiodothyronine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris/HCl, 0.25 M sucrose, 1 mM EDTA
PH: 7
Temperature: 37
uniprot:P24389 [Iopanoic acid]

3,3',5'_Triiodothyronine + Reduced_acceptor -> 3,3'_Diiodothyronine + H+ + Acceptor + I_		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.02 M Tris/HCl, 0.25 M sucrose, 1 mM EDTA
PH: 7
Temperature: 37
uniprot:P24389 [Iopanoic acid]

3,3',5'_Triiodothyronine + Reduced_acceptor -> H+ + 3,3'_Diiodothyronine + I_ + Acceptor		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.01 M sodium phosphate, 0.15 M NaCl
PH: 7.4
Temperature: 37
uniprot:P24389 [Iopanoic acid]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> Acceptor + H+ + 3,3'_Diiodothyronine + I_		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.01 M sodium phosphate, 0.15 M NaCl
PH: 7.4
Temperature: 37
uniprot:P24389 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> H+ + I_ + 3,3'_Diiodothyronine + Acceptor		 (( (V * S) ) / (Km + S)) buffer: 0.1 M Tris
PH: 7.4
Temperature: 37
- [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> I_ + 3,3'_Diiodothyronine + H+ + Acceptor		 (( (V * S) ) / (Km + S)) buffer: 0.1 M Tris
PH: 7.4
Temperature: 37
uniprot:P49895 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> 3,3'_Diiodothyronine + H+ + I_ + Acceptor		 (( (V * S) ) / (Km + S)) buffer: 0.1 M Tris
PH: 7.4
Temperature: 37
uniprot:O42411 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> Acceptor + H+ + I_ + 3,3'_Diiodothyronine		 (( (V * S) ) / (Km + S)) buffer: 0.1 M Tris
PH: 7.4
Temperature: 37
uniprot:Q9BDR5 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

Reduced_acceptor + 3,3',5'_Triiodothyronine -> H+ + 3,3'_Diiodothyronine + Acceptor + I_		 (( (V * S) ) / (Km + S)) buffer: 0.1 M Tris
PH: 7.4
Temperature: 37
uniprot:Q61153 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> H+ + 3,3'_Diiodothyronine + Acceptor + I_		 (( (V * S) ) / (Km + S)) buffer: 0.1 M Tris
PH: 7.4
Temperature: 37
uniprot:Q6QN13 [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> Acceptor + H+ + I_ + 3,3'_Diiodothyronine		 (( (V * S) ) / (Km + S)) buffer: 0.1 M Tris
PH: 7.4
Temperature: 37
- [thyroxine 5'-deiodinase(Enzyme) wildtype isoenzyme type I]

3,3',5'_Triiodothyronine + Reduced_acceptor -> H+ + Acceptor + I_ + 3,3'_Diiodothyronine		 (( (V * S) ) / (Km + S)) buffer: 0.1 M Tris
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6688 thyronamine and iodothyronamine metabolism
BioCyc:META_PWY-6261 thyroid hormone metabolism II (via conjugation and/or degradation)
BioCyc:META_PWY-6688 thyronamine and iodothyronamine metabolism
BioCyc:MOUSE_PWY-6260 thyroid hormone metabolism I (via deiodination)
BioCyc:HUMAN_PWY-6261 thyroid hormone metabolism II (via conjugation and/or degradation)
BioCyc:HUMAN_PWY-6260 thyroid hormone metabolism I (via deiodination)
BioCyc:META_PWY-6260 thyroid hormone metabolism I (via deiodination)
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-BTA-209968 Thyroxine biosynthesis
Reactome:R-CFA-1430728 Metabolism
Reactome:R-CFA-350864 Regulation of thyroid hormone activity
Reactome:R-MMU-1430728 Metabolism
Reactome:R-CFA-71291 Amino acid and derivative metabolism
Reactome:R-CFA-209968 Thyroxine biosynthesis
Reactome:R-HSA-209776 Metabolism of amine-derived hormones
Reactome:R-MMU-209776 Metabolism of amine-derived hormones
Reactome:R-RNO-209776 Metabolism of amine-derived hormones
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-209776 Metabolism of amine-derived hormones
Reactome:R-BTA-350864 Regulation of thyroid hormone activity
Reactome:R-CFA-209776 Metabolism of amine-derived hormones
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-209968 Thyroxine biosynthesis
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-209968 Thyroxine biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-RNO-209968 Thyroxine biosynthesis
Reactome:R-SSC-71291 Amino acid and derivative metabolism
Reactome:R-SSC-209968 Thyroxine biosynthesis
Reactome:R-BTA-209776 Metabolism of amine-derived hormones
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-350864 Regulation of thyroid hormone activity
Reactome:R-MMU-350864 Regulation of thyroid hormone activity
Reactome:R-RNO-350864 Regulation of thyroid hormone activity
Reactome:R-SSC-350864 Regulation of thyroid hormone activity