EC: 1.1.1.239

3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+) (3alpha(or 17beta)-hydroxysteroid:NAD+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P42330 [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype AKR1C3]

5alpha_Androstane_17beta_ol_3_one + NADP+ -> H+ + NADPH + 5alpha_Androstane_3,17_dione		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 25
uniprot:P42330 [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype AKR1C3]

5alpha_Androstane_3,17_dione + NADPH + H+ -> 5alpha_Androstane_17beta_ol_3_one + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 25
uniprot:P42330 [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype AKR1C3]

5alpha_Androstane_3alpha_ol_17_one + H+ + NADPH -> NADP+ + 5alpha_Androstane_3alpha,17beta_diol		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 25
uniprot:P42330 [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype AKR1C3]

5alpha_Androstane_3alpha,17beta_diol + NADP+ -> NADPH + H+ + 5alpha_Androstane_3alpha_ol_17_one		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

H+ + 3alpha_Hydroxy_5beta_androstan_17_one + NADH -> 5beta_Androstane_3alpha,17beta_diol + NAD+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

NAD+ + 5beta_Androstane_3alpha,17beta_diol -> H+ + 3alpha_Hydroxy_5beta_androstan_17_one + NADH		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype]

NAD+ + Testosterone -> Androst_4_ene_3,17_dione + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.18 M Bicine
PH: 9
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

Estradiol_17beta + NAD+ -> H+ + Estrone + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

NADH + H+ + 3alpha_Hydroxy_5beta_androstan_17_one -> 5beta_Androstane_3alpha,17beta_diol + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

NADH + H+ + Estrone_3_sulfate -> NAD+ + Estradiol_17beta_3_sulfate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

NAD+ + Testosterone -> H+ + NADH + Androst_4_ene_3,17_dione		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

NAD+ + 5beta_Androstane_3alpha,17beta_diol -> 3alpha_Hydroxy_5beta_androstan_17_one + H+ + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

5beta_Androstane_17beta_ol_3_one + NAD+ -> NADH + 5beta_Androstane_3,17_dione + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

NAD+ + 5alpha_Androstane_3alpha,17beta_diol -> 5alpha_Androstan_3alpha_ol_17_one + H+ + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype NAD-dependent]

5alpha_Androstane_17beta_ol_3_one + NAD+ -> H+ + 5alpha_Androstane_3,17_dione + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7.5
Temperature: 25
- [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype]

Testosterone + NAD+ -> H+ + Androst_4_ene_3,17_dione + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.18 M Bicine
PH: 9
Temperature: 25
uniprot:P42330 [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype 3alpha-HSD Type 2]

5alpha_Androstane_3alpha,17beta_diol + NADP+ -> 5alpha_Androstane_3alpha_ol_17_one + H+ + NADPH		 (( (( (E * kcat) ) * A) ) / (A + Km)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:P42330 [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype 3alpha-HSD Type 2]

NADP+ + 5alpha_Androstane_3alpha,17beta_diol -> H+ + 5alpha_Androstane_3alpha_ol_17_one + NADPH		 (( (( (E * kcat) ) * A) ) / (A + Km)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 25
uniprot:P42330 [3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)(Enzyme) wildtype 3alpha-HSD Type 2]

NADP+ + 5alpha_Androstane_3alpha,17beta_diol -> 5alpha_Androstane_3alpha_ol_17_one + H+ + NADPH		 (( (( (E * kcat) ) * A) ) / (A + Km)) buffer: 100 mM Potassium phosphate
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-7455 allopregnanolone biosynthesis
BioCyc:HUMAN_ILEUDEG-PWY isoleucine degradation
BioCyc:HUMAN_PWY66-378 androgen biosynthesis
BioCyc:META_FAO-PWY fatty acid β-oxidation I
BioCyc:META_ILEUDEG-PWY L-isoleucine degradation I
BioCyc:MOUSE_PWY3DJ-0 isoleucine degradation
BioCyc:META_PWY66-378 androgen biosynthesis
BioCyc:MOUSE_PWY-5109 2-methylbutyrate biosynthesis
BioCyc:HUMAN_FAO-PWY fatty acid β-oxidation
BioCyc:META_PWY-7455 allopregnanolone biosynthesis
BioCyc:MOUSE_FAO-PWY fatty acid β-oxidation I
BioCyc:MOUSE_PWY-5136 fatty acid β-oxidation II (core pathway)
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-CFA-1430728 Metabolism
Reactome:R-CFA-556833 Metabolism of lipids
Reactome:R-CFA-75105 Fatty acyl-CoA biosynthesis
Reactome:R-HSA-75105 Fatty acyl-CoA biosynthesis
Reactome:R-HSA-70895 Branched-chain amino acid catabolism
Reactome:R-HSA-5362517 Signaling by Retinoic Acid
Reactome:R-HSA-72306 tRNA processing
Reactome:R-HSA-6785470 tRNA processing in the mitochondrion
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-75105 Fatty acyl-CoA biosynthesis
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-MMU-193144 Estrogen biosynthesis
Reactome:R-MMU-70895 Branched-chain amino acid catabolism
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-196071 Metabolism of steroid hormones
Reactome:R-RNO-70895 Branched-chain amino acid catabolism
Reactome:R-BTA-70895 Branched-chain amino acid catabolism
Reactome:R-CFA-8978868 Fatty acid metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-HSA-193144 Estrogen biosynthesis
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-196071 Metabolism of steroid hormones
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-5365859 RA biosynthesis pathway
Reactome:R-HSA-8953854 Metabolism of RNA
Reactome:R-HSA-6787450 tRNA modification in the mitochondrion
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-75105 Fatty acyl-CoA biosynthesis
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-RNO-193144 Estrogen biosynthesis
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-196071 Metabolism of steroid hormones
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-72312 rRNA processing
Reactome:R-HSA-8868766 rRNA processing in the mitochondrion
WikiPathways:WP5280 Glucocorticoid biosynthesis
WikiPathways:WP5368 Sulfatase and aromatase pathway
WikiPathways:WP5320 Male steroid hormones in cardiomyocyte energy metabolism
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
WikiPathways:WP4524 Alternative pathway of fetal androgen synthesis
PathBank:SMP0120440 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120444 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120516 Maple Syrup Urine Disease
PathBank:SMP0120589 Isovaleric Acidemia
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0000544 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0000568 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0063612 Fatty Acid Elongation In Mitochondria
PathBank:SMP0120439 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120443 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120459 beta-Ketothiolase Deficiency
PathBank:SMP0120503 Isovaleric Aciduria
PathBank:SMP0120523 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120588 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120608 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0120632 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0000054 Fatty Acid Elongation in Mitochondria
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0063641 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0063689 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120441 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120525 Methylmalonic Aciduria
PathBank:SMP0120537 Propionic Acidemia
PathBank:SMP0120586 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000650 Doxorubicin Metabolism Pathway
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0087260 Retinol Metabolism
PathBank:SMP0120442 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120587 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000480 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000635 Valproic Acid Metabolism Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway