EC: 1.14.99.1

prostaglandin-endoperoxide synthase ((5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate,hydrogen-donor:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P35354 [Heme]

15_HPETE + 2 Guaiacol -> 15_HETE + H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [Heme]

2 Guaiacol + trans_5_Phenyl_4_pentenyl_hydroperoxide -> H2O + 5_Phenyl_4E_pentenol + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [Heme]

trans_5_Phenyl_4_pentenyl_hydroperoxide + 2 Guaiacol -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O + 5_Phenyl_4E_pentenol		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [Heme]

trans_5_Phenyl_4_pentenyl_hydroperoxide + 2 Guaiacol -> H2O + 5_Phenyl_4E_pentenol + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [Heme]

2 Guaiacol + trans_5_Phenyl_4_pentenyl_hydroperoxide -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone + 5_Phenyl_4E_pentenol		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [Heme]

2 Guaiacol + trans_5_Phenyl_4_pentenyl_hydroperoxide -> 3,3'_Dimethoxydipheno_4,4'_quinone + 5_Phenyl_4E_pentenol + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [Heme]

2 Guaiacol + H2O2 -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

Docosahexaenoic_acid + Reduced_acceptor + 2 O2 -> Acceptor + Unknown + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PW89 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-2]

Reduced_acceptor + 2 O2 + Docosahexaenoic_acid -> Unknown + Acceptor + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [Heme]

Reduced_acceptor + Arachidonate + 2 O2 -> H2O + Acceptor + Prostaglandin_H2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 5 mM D-tryptophan
PH: 7.2
Temperature: 30
uniprot:P35354 [Heme]

2 Guaiacol + trans_5_Phenyl_4_pentenyl_hydroperoxide -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O + 5_Phenyl_4E_pentenol		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype COX-1]

2 O2 + Reduced_acceptor + Arachidonate -> H2O + Prostaglandin_H2 + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

Arachidonate + Reduced_acceptor + 2 O2 -> Prostaglandin_H2 + Acceptor + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PW89 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-2]

Reduced_acceptor + 2 O2 + Arachidonate -> H2O + Prostaglandin_H2 + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PTN3 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-1]

2 O2 + Reduced_acceptor + Arachidonate -> H2O + Acceptor + Prostaglandin_H2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype COX-1]

Reduced_acceptor + 8,11,14_Eicosatrienoate + 2 O2 -> Unknown + H2O + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

Reduced_acceptor + 8,11,14_Eicosatrienoate + 2 O2 -> H2O + Unknown + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PTN3 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-1]

Reduced_acceptor + 2 O2 + 8,11,14_Eicosatrienoate -> Acceptor + H2O + Unknown		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PW89 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-2]

Reduced_acceptor + 2 O2 + 8,11,14_Eicosatrienoate -> H2O + Unknown + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype COX-1]

Eicosapentaenoic_acid + 2 O2 + Reduced_acceptor -> Acceptor + Unknown + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

Arachidonate + 2 O2 -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 5 mM D-tryptophan, 5 µM heme
PH: 7.2
Temperature: 22
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) mutant hPGHS-2 (V509E)]

2 O2 + Arachidonate -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 5 mM D-tryptophan, 5 µM heme
PH: 7.2
Temperature: 22
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) mutant hPGHS-2 (V509I)]

2 O2 + Arachidonate -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 5 mM D-tryptophan, 5 µM heme
PH: 7.2
Temperature: 22
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) mutant hPGHS-2 (V509K)]

2 O2 + Arachidonate -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 5 mM D-tryptophan, 5 µM heme
PH: 7.2
Temperature: 22
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) mutant hPGHS-2 (V509A)]

Arachidonate + 2 O2 -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 5 mM D-tryptophan, 5 µM heme
PH: 7.2
Temperature: 22
uniprot:P23219 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-1]

2 O2 + Arachidonate -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 5 mM D-tryptophan, 5 µM heme
PH: 7.2
Temperature: 22
uniprot:P35354 [Heme]

Arachidonate + 2 O2 -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol
PH: 7.2
Temperature: 30
uniprot:P35354 [Heme]

Arachidonate + 2 O2 -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol
PH: 7.2
Temperature: 30
uniprot:P35354 [Heme]

Arachidonate + 2 O2 -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol
PH: 7.2
Temperature: 30
uniprot:P35354 [Heme]

2 O2 + Arachidonate -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol
PH: 7.2
Temperature: 30
uniprot:P35354 [Heme]

Arachidonate + 2 O2 -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol
PH: 7.2
Temperature: 30
uniprot:P35354 [Heme]

Arachidonate + 2 O2 -> Prostaglandin_G2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol
PH: 7.2
Temperature: 30
uniprot:P35354 [Heme]

H2O2 + 2 Guaiacol -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [Heme]

H2O2 + 2 Guaiacol -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P35354 [Heme]

H2O2 + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 23
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype COX-1]

2 O2 + Reduced_acceptor + alpha_Linolenate -> Unknown + H2O + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

2 O2 + alpha_Linolenate + Reduced_acceptor -> Acceptor + H2O + Unknown		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PTN3 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-1]

Reduced_acceptor + 2 O2 + alpha_Linolenate -> Unknown + Acceptor + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PW89 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-2]

alpha_Linolenate + Reduced_acceptor + 2 O2 -> Unknown + H2O + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype COX-1]

2 O2 + gamma_Linolenic_acid + Reduced_acceptor -> H2O + Unknown + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

gamma_Linolenic_acid + 2 O2 + Reduced_acceptor -> Unknown + Acceptor + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype COX-1]

2 O2 + Reduced_acceptor + Dihomolinoleate -> H2O + Unknown + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PTN3 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-1]

2 O2 + Reduced_acceptor + gamma_Linolenic_acid -> H2O + Acceptor + Unknown		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

2 O2 + Dihomolinoleate + Reduced_acceptor -> Acceptor + H2O + Unknown		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PW89 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-2]

Reduced_acceptor + 2 O2 + gamma_Linolenic_acid -> Unknown + Acceptor + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

2 O2 + Eicosapentaenoic_acid + Reduced_acceptor -> Unknown + H2O + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PTN3 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-1]

2 O2 + Eicosapentaenoic_acid + Reduced_acceptor -> H2O + Unknown + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PW89 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-2]

Reduced_acceptor + Eicosapentaenoic_acid + 2 O2 -> H2O + Unknown + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype COX-1]

Docosahexaenoic_acid + 2 O2 + Reduced_acceptor -> Unknown + Acceptor + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PTN3 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-1]

2 O2 + Reduced_acceptor + Docosahexaenoic_acid -> H2O + Acceptor + Unknown		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype COX-1]

Reduced_acceptor + Docosatetraenoic_acid + 2 O2 -> H2O + Acceptor + Unknown		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPGHS-2]

2 O2 + Reduced_acceptor + Docosatetraenoic_acid -> H2O + Unknown + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PW89 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-2]

Reduced_acceptor + 2 O2 + Docosatetraenoic_acid -> Unknown + H2O + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:Q9PTN3 [prostaglandin-endoperoxide synthase(Enzyme) wildtype tPGHS-1]

2 O2 + Reduced_acceptor + Docosatetraenoic_acid -> Unknown + H2O + Acceptor		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 1 mM phenol, 0.025% Tween 20, 1 µM heme
PH: 7.2
Temperature: 25
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype hPTGS2]

Reduced_acceptor + 2 O2 + Arachidonate -> H2O + Prostaglandin_H2 + Acceptor		 (( (Vmax * S) ) / (S + Km)) buffer: 0.1 M Tris-Cl, 1 mM phenol, 1.7 µg/µl hemoglobin
PH: 8
Temperature: 37
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) mutant hPTGS2 (V511A)]

2 O2 + Arachidonate + Reduced_acceptor -> Prostaglandin_H2 + Acceptor + H2O		 (( (Vmax * S) ) / (S + Km)) buffer: 0.1 M Tris-Cl, 1 mM phenol, 1.7 µg/µl hemoglobin
PH: 8
Temperature: 37
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype FLAG huCOX-2]

Reduced_acceptor + Arachidonate + 2 O2 -> Acceptor + Prostaglandin_H2 + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 1 mM phenol, 5 µM hematin
PH: 8
Temperature: 37
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype His-tagged]

2 O2 + Arachidonate + Reduced_acceptor -> H2O + Acceptor + Prostaglandin_H2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 1 mM phenol, 5 µM hematin
PH: 8
Temperature: 37
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype His-tagged]

Reduced_acceptor + 2 O2 + Arachidonate -> Prostaglandin_H2 + H2O + Acceptor		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 1 mM phenol, 5 µM hematin
PH: 8
Temperature: 37
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype His-tagged]

2 O2 + Arachidonate + Reduced_acceptor -> Prostaglandin_H2 + H2O + Acceptor		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 1 mM phenol, 5 µM hematin
PH: 8
Temperature: 37
uniprot:P35354 [prostaglandin-endoperoxide synthase(Enzyme) wildtype His-tagged]

2 O2 + Reduced_acceptor + Arachidonate -> Prostaglandin_H2 + Acceptor + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 1 mM phenol, 5 µM hematin
PH: 8
Temperature: 37
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype]

2 Guaiacol + 15_HPETE -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone + 15_HETE		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype]

2 Guaiacol + Ethyl_hydroperoxide -> 3,3'_Dimethoxydipheno_4,4'_quinone + Ethanol + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype]

Guaiacol + t_Butylhydroperoxide -> 3,3'_Dimethoxydipheno_4,4'_quinone + t_Butanol + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) wildtype]

H2O2 + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294A/F409A]

15_HPETE + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone + 15_HETE		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant F409A]

15_HPETE + 2 Guaiacol -> 15_HETE + 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294A/L295A]

2 Guaiacol + 15_HPETE -> 15_HETE + H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant V291A]

2 Guaiacol + 15_HPETE -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone + 15_HETE		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant V291A/L294A]

2 Guaiacol + 15_HPETE -> 15_HETE + H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L295A/L408A]

2 Guaiacol + 15_HPETE -> 15_HETE + 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294A]

15_HPETE + 2 Guaiacol -> 15_HETE + H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L408A]

15_HPETE + 2 Guaiacol -> 3,3'_Dimethoxydipheno_4,4'_quinone + 15_HETE + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L295A]

15_HPETE + 2 Guaiacol -> 15_HETE + H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant Q203V]

2 Guaiacol + 15_HPETE -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone + 15_HETE		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294D]

15_HPETE + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone + 15_HETE		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant Q203N]

2 Guaiacol + 15_HPETE -> 15_HETE + 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294S]

2 Guaiacol + 15_HPETE -> 3,3'_Dimethoxydipheno_4,4'_quinone + 15_HETE + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant V291A/L294A/L295A]

2 Guaiacol + 15_HPETE -> 15_HETE + H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294E]

2 Guaiacol + 15_HPETE -> 3,3'_Dimethoxydipheno_4,4'_quinone + 15_HETE + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294G]

15_HPETE + 2 Guaiacol -> 15_HETE + H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant V291A]

H2O2 + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294A]

2 Guaiacol + H2O2 -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294G]

2 Guaiacol + H2O2 -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant F409A]

H2O2 + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant V291A/L294A]

2 Guaiacol + H2O2 -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant V291A/L294A/L295A]

2 Guaiacol + H2O2 -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L295A/L408A]

2 Guaiacol + H2O2 -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294A/F409A]

2 Guaiacol + H2O2 -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294A/L295A]

H2O2 + 2 Guaiacol -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L408A]

2 Guaiacol + H2O2 -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L295A]

H2O2 + 2 Guaiacol -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294S]

H2O2 + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant Q203V]

H2O2 + 2 Guaiacol -> 3,3'_Dimethoxydipheno_4,4'_quinone + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant L294D]

H2O2 + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P05979 [prostaglandin-endoperoxide synthase(Enzyme) mutant Q203N]

H2O2 + 2 Guaiacol -> H2O + 3,3'_Dimethoxydipheno_4,4'_quinone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mMTris-HCl, 100 mM NaCl
PH: 8
Temperature: 4
uniprot:P35354 [Heme]

Arachidonate + Reduced_acceptor + 2 O2 -> Prostaglandin_H2 + Acceptor + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-maleate, 0.1% Tween-20, 0.2% (w:v) gelatin
PH: 6.5
Temperature: 25
uniprot:P35354 [Hematin]

Reduced_acceptor + 2 O2 + Arachidonate -> H2O + Acceptor + Prostaglandin_H2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 5 mM EDTA, 0.1 % w/v Tween-20
PH: 8
Temperature: 25
uniprot:P05979 [Hematin]

Reduced_acceptor + Arachidonate + 2 O2 -> H2O + Prostaglandin_H2 + Acceptor		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 5 mM EDTA, 0.1 % w/v Tween-20
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY66-394 aspirin triggered resolvin E biosynthesis
BioCyc:HUMAN_PWY66-395 aspirin triggered resolvin D biosynthesis
BioCyc:HUMAN_PWY66-393 aspirin-triggered lipoxin biosynthesis
BioCyc:META_PWY66-393 aspirin-triggered lipoxin biosynthesis
BioCyc:META_PWY66-394 aspirin triggered resolvin E biosynthesis
BioCyc:META_PWY66-374 C20 prostanoid biosynthesis
BioCyc:META_PWY66-395 aspirin triggered resolvin D biosynthesis
BioCyc:HUMAN_PWY66-374 C20 prostanoid biosynthesis
BioCyc:MOUSE_PWY3DJ-35583 biosynthesis of prostaglandins
Reactome:R-CFA-1430728 Metabolism
Reactome:R-CFA-556833 Metabolism of lipids
Reactome:R-GGA-8978868 Fatty acid metabolism
Reactome:R-GGA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-GGA-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-GGA-9027604 Biosynthesis of electrophilic -3 PUFA oxo-derivatives
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-MMU-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-RNO-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-140180 COX reactions
Reactome:R-CFA-8978868 Fatty acid metabolism
Reactome:R-CFA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-GGA-2142753 Arachidonate metabolism
Reactome:R-GGA-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-GGA-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-GGA-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-HSA-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-HSA-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-HSA-449147 Signaling by Interleukins
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-MMU-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-RNO-2142753 Arachidonate metabolism
Reactome:R-RNO-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-RNO-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-RNO-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-RNO-9027604 Biosynthesis of electrophilic -3 PUFA oxo-derivatives
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-CFA-211859 Biological oxidations
Reactome:R-GGA-1430728 Metabolism
Reactome:R-GGA-556833 Metabolism of lipids
Reactome:R-GGA-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-HSA-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-HSA-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-HSA-9027604 Biosynthesis of electrophilic -3 PUFA oxo-derivatives
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-140180 COX reactions
Reactome:R-HSA-168256 Immune System
Reactome:R-HSA-6783783 Interleukin-10 signaling
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-140180 COX reactions
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-RNO-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-CFA-2142753 Arachidonate metabolism
Reactome:R-CFA-211945 Phase I - Functionalization of compounds
Reactome:R-CFA-140180 COX reactions
Reactome:R-GGA-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-HSA-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-HSA-1280215 Cytokine Signaling in Immune system
Reactome:R-MMU-2142753 Arachidonate metabolism
Reactome:R-MMU-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-MMU-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-MMU-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-MMU-9027604 Biosynthesis of electrophilic -3 PUFA oxo-derivatives
WikiPathways:WP4347 Eicosanoid metabolism via cyclooxygenases (COX)
WikiPathways:WP4586 Metabolism of alpha-linolenic acid
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000601 Etoposide Metabolism Pathway
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0120884 Succinate Signalling During Inflammation
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000644 Celecoxib Metabolism Pathway
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0063595 Arachidonic Acid Metabolism
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000442 Etoposide Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000590 Ibuprofen Metabolism Pathway
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0120510 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway
PathBank:SMP0084634 Succinate Signalling During Inflammation