EC: 1.14.16.2

tyrosine 3-monooxygenase (L-tyrosine,tetrahydrobiopterin:oxygen oxidoreductase (3-hydroxylating))

enzyme lambda metabolic reaction experiment
- [6,7-Dimethyl-tetrahydropteridine]

O2 + Tetrahydrobiopterin + L_Tyrosine -> L_Dopa + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10000 U/ml Catalase, 5 U/ml Dihydropteridine reductase, 0.43 mM NADPH
PH: 7
Temperature: 37
- [6,7-Dimethyl-tetrahydropteridine]

L_Tyrosine + Tetrahydrobiopterin + O2 -> L_Dopa + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 10000 U/ml Catalase, 5 U/ml Dihydropteridine reductase, 0.43 mM NADPH
PH: 7
Temperature: 37
- [6,7-Dimethyl-tetrahydropteridine]

O2 + Tyramine -> Dopamine + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10000 U/ml Catalase, 5 U/ml Dihydropteridine reductase, 0.43 mM NADPH
PH: 7
Temperature: 37
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (V * A) ) / (Ka + A)) buffer: 50 mM HEPES-NaOH, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (V * B) ) / (Kb + B)) buffer: 50 mM HEPES-NaOH, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant F300A]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (V * A) ) / (Ka + A)) buffer: 50 mM HEPES-NaOH, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant F300A]

O2 + 6_Methyltetrahydropterin + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (V * B) ) / (Kb + B)) buffer: 50 mM HEPES-NaOH, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant F309A]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (V * A) ) / (Ka + A)) buffer: 50 mM HEPES-NaOH, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant F309A]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (V * B) ) / (Kb + B)) buffer: 50 mM HEPES-NaOH, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (V * A) ) / (Ka + A)) buffer: 80 mM HEPES-NaOH, 100 µg/ml catalase, 200 µM NADH, 10 µM ferrous ammonium sulfate, 0.1 U/ml sheep dihydropterin reductase
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant F309A]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (V * A) ) / (Ka + A)) buffer: 80 mM HEPES-NaOH, 100 µg/ml catalase, 200 µM NADH, 10 µM ferrous ammonium sulfate, 0.1 U/ml sheep dihydropterin reductase
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant F300A]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (V * A) ) / (Ka + A)) buffer: 80 mM HEPES-NaOH, 100 µg/ml catalase, 200 µM NADH, 10 µM ferrous ammonium sulfate, 0.1 U/ml sheep dihydropterin reductase
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (V * B) ) / (Kb + B)) buffer: 80 mM HEPES-NaOH, 100 µg/ml catalase, 200 µM NADH, 10 µM ferrous ammonium sulfate, 0.1 U/ml sheep dihydropterin reductase
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant F300A]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (V * B) ) / (Kb + B)) buffer: 80 mM HEPES-NaOH, 100 µg/ml catalase, 200 µM NADH, 10 µM ferrous ammonium sulfate, 0.1 U/ml sheep dihydropterin reductase
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant F309A]

L_Tyrosine + 6_Methyltetrahydropterin + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (V * B) ) / (Kb + B)) buffer: 80 mM HEPES-NaOH, 100 µg/ml catalase, 200 µM NADH, 10 µM ferrous ammonium sulfate, 0.1 U/ml sheep dihydropterin reductase
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S8A]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S31E]

O2 + 6_Methyltetrahydropterin + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19A]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S31A]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S40A]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S8E]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19E]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S8E]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19E]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S31A]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19A]

L_Tyrosine + 6_Methyltetrahydropterin + O2 -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S8A]

O2 + 6_Methyltetrahydropterin + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant TyrHpS31]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19E/S40E]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S40E]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S8A]

O2 + Tetrahydrobiopterin + L_Tyrosine -> 6,7_Dihydrobiopterin + H2O + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

L_Tyrosine + Tetrahydrobiopterin + O2 -> L_Dopa + 6,7_Dihydrobiopterin + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S8E]

O2 + L_Tyrosine + Tetrahydrobiopterin -> 6,7_Dihydrobiopterin + H2O + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19A]

Tetrahydrobiopterin + O2 + L_Tyrosine -> L_Dopa + H2O + 6,7_Dihydrobiopterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant TyrHpS31]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19E/S40E]

L_Tyrosine + 6_Methyltetrahydropterin + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S40A]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S40E]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S31E]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

L_Tyrosine + O2 + Tetrahydrobiopterin -> H2O + 6,7_Dihydrobiopterin + L_Dopa		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S8A]

L_Tyrosine + Tetrahydrobiopterin + O2 -> 6,7_Dihydrobiopterin + H2O + L_Dopa		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S31A]

O2 + L_Tyrosine + Tetrahydrobiopterin -> H2O + 6,7_Dihydrobiopterin + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S40E]

L_Tyrosine + Tetrahydrobiopterin + O2 -> H2O + 6,7_Dihydrobiopterin + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant TyrHpS31]

Tetrahydrobiopterin + L_Tyrosine + O2 -> H2O + L_Dopa + 6,7_Dihydrobiopterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19E/S40E]

O2 + Tetrahydrobiopterin + L_Tyrosine -> L_Dopa + 6,7_Dihydrobiopterin + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S31E]

O2 + Tetrahydrobiopterin + L_Tyrosine -> 6,7_Dihydrobiopterin + H2O + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19E]

Tetrahydrobiopterin + L_Tyrosine + O2 -> 6,7_Dihydrobiopterin + H2O + L_Dopa		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S40A]

O2 + Tetrahydrobiopterin + L_Tyrosine -> 6,7_Dihydrobiopterin + L_Dopa + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S40E]

Tetrahydrobiopterin + O2 + L_Tyrosine -> 6,7_Dihydrobiopterin + L_Dopa + H2O		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S8E]

O2 + L_Tyrosine + Tetrahydrobiopterin -> 6,7_Dihydrobiopterin + H2O + L_Dopa		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant TyrHpS31]

L_Tyrosine + O2 + Tetrahydrobiopterin -> H2O + L_Dopa + 6,7_Dihydrobiopterin		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19E/S40E]

O2 + L_Tyrosine + Tetrahydrobiopterin -> H2O + L_Dopa + 6,7_Dihydrobiopterin		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19A]

Tetrahydrobiopterin + L_Tyrosine + O2 -> H2O + L_Dopa + 6,7_Dihydrobiopterin		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S31A]

Tetrahydrobiopterin + O2 + L_Tyrosine -> H2O + 6,7_Dihydrobiopterin + L_Dopa		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S31E]

O2 + L_Tyrosine + Tetrahydrobiopterin -> L_Dopa + 6,7_Dihydrobiopterin + H2O		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S19E]

L_Tyrosine + Tetrahydrobiopterin + O2 -> L_Dopa + 6,7_Dihydrobiopterin + H2O		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant S40A]

Tetrahydrobiopterin + O2 + L_Tyrosine -> H2O + L_Dopa + 6,7_Dihydrobiopterin		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 50 mM Hepes, 100 µg/ml catalase, 10 µM ferrous ammonium sulfate, 1 mM dithiothreitol
PH: 7
Temperature: 30
uniprot:P04177 [Fe2+]

Tetrahydrobiopterin + O2 + L_Tyrosine -> 4a_Hydroxytetrahydrobiopterin + L_Dopa		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 7.1
Temperature: 30
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 7.1
Temperature: 30
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 6
Temperature: 30
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 7.1
Temperature: 30
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 6
Temperature: 30
uniprot:P04177 [Fe2+]

L_Tyrosine + 6_Methyltetrahydropterin + O2 -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 6
Temperature: 30
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 7.1
Temperature: 30
uniprot:P04177 [Fe2+]

L_Tyrosine + 6_Methyltetrahydropterin + O2 -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 6
Temperature: 30
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 7.1
Temperature: 30
uniprot:P04177 [Fe2+]

L_Tyrosine + O2 + Tetrahydrobiopterin -> L_Dopa + 4a_Hydroxytetrahydrobiopterin		 (( (kcat * A) ) / (Km + A)) buffer: 0.2 M Tris-HCl, 0.1 M MES, 0.1 M Sodium acetate, 2.5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 7.1
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant Y371F]

6_Methyltetrahydropterin + L_Phenylalanine + O2 -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (kcat * A) ) / (Km + A)) buffer: 80 mM HEPES-NaOH, 5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 7.1
Temperature: 30
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + O2 + 6_Methyltetrahydropterin -> L_Tyrosine + 6_Methyldihydropterin + H2O		 (( (kcat * A) ) / (Km + A)) buffer: 80 mM HEPES-NaOH, 5 mM Dithiothreitol, 60 µg/ml Catalase
PH: 7.1
Temperature: 30
uniprot:P04177 [Fe2+]

L_Tyrosine + 6_Methyltetrahydropterin + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM Hepes/NaOH, 100 µM NADH, 170 µg/ml Catalase, 0.2-0.4 units/ml Sheep dihydropteridine reductase
PH: 7
Temperature: 25
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM Hepes/NaOH, 100 µM NADH, 170 µg/ml Catalase, 0.2-0.4 units/ml Sheep dihydropteridine reductase
PH: 7
Temperature: 25
uniprot:P04177 [Fe2+]

O2 + 6_Methyltetrahydropterin + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/NaOH, 1 mM Dithiothreitol, 125 µg/ml Catalase
PH: 7.1
Temperature: 25
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/NaOH, 1 mM Dithiothreitol, 125 µg/ml Catalase
PH: 7.1
Temperature: 25
uniprot:P04177 [Fe2+]

L_Tyrosine + 6_Methyltetrahydropterin + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/NaOH, 1 mM Dithiothreitol, 125 µg/ml Catalase
PH: 7.1
Temperature: 25
uniprot:P04177 [Fe2+]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/NaOH, 1 mM Dithiothreitol, 125 µg/ml Catalase
PH: 7.1
Temperature: 25
uniprot:P04177 [Fe2+]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/NaOH, 1 mM Dithiothreitol, 125 µg/ml Catalase
PH: 7.1
Temperature: 25
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/NaOH, 1 mM Dithiothreitol, 125 µg/ml Catalase
PH: 7.1
Temperature: 25
uniprot:P04177 [Fe2+]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/NaOH, 1 mM Dithiothreitol, 125 µg/ml Catalase
PH: 7.1
Temperature: 25
uniprot:P04177 [Fe2+]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/NaOH, 1 mM Dithiothreitol, 125 µg/ml Catalase
PH: 7.1
Temperature: 25
uniprot:P04176 [tyrosine 3-monooxygenase(Enzyme) mutant V379D]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 200 µM NADH, 10 µM Ferrous ammonium sulfate, 0.2 units/ml Sheep dyhydropteridine reductase
PH: 6.7
Temperature: 32
uniprot:P04176 [tyrosine 3-monooxygenase(Enzyme) wildtype]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 200 µM NADH, 10 µM Ferrous ammonium sulfate, 0.2 units/ml Sheep dyhydropteridine reductase
PH: 6.7
Temperature: 32
uniprot:P04176 [tyrosine 3-monooxygenase(Enzyme) mutant H264Q]

6_Methyltetrahydropterin + L_Tyrosine + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 200 µM NADH, 10 µM Ferrous ammonium sulfate, 0.2 units/ml Sheep dyhydropteridine reductase
PH: 6.7
Temperature: 32
uniprot:P04176 [tyrosine 3-monooxygenase(Enzyme) mutant Y277H]

L_Tyrosine + 6_Methyltetrahydropterin + O2 -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 200 µM NADH, 10 µM Ferrous ammonium sulfate, 0.2 units/ml Sheep dyhydropteridine reductase
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant Q310H]

O2 + L_Tyrosine + 6_Methyltetrahydropterin -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) wildtype]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04176 [tyrosine 3-monooxygenase(Enzyme) mutant H264Q/V379D]

6_Methyltetrahydropterin + O2 + L_Tyrosine -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 200 µM NADH, 10 µM Ferrous ammonium sulfate, 0.2 units/ml Sheep dyhydropteridine reductase
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant H323Y]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04177 [tyrosine 3-monooxygenase(Enzyme) mutant D425V]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> L_Dopa + 4_Hydroxy_6_methyltetrahydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 200 µM NADH, 10 µM Ferrous ammonium sulfate, 0.2 units/ml Sheep dyhydropteridine reductase
PH: 6.7
Temperature: 32
uniprot:P04176 [tyrosine 3-monooxygenase(Enzyme) mutant H264Q/Y277H/V379D]

O2 + 6_Methyltetrahydropterin + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 200 µM NADH, 10 µM Ferrous ammonium sulfate, 0.2 units/ml Sheep dyhydropteridine reductase
PH: 6.7
Temperature: 32
- [tyrosine 3-monooxygenase(Enzyme) wildtype]

O2 + 6_Methyltetrahydropterin + L_Tyrosine -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 0.016 M KH2PO4/K2HPO4, 1.67 mM Sodium ascorbate
PH: 7
Temperature: 30
- [tyrosine 3-monooxygenase(Enzyme) wildtype]

L_Tyrosine + O2 + 6_Methyltetrahydropterin -> 4_Hydroxy_6_methyltetrahydropterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 0.016 M KH2PO4/K2HPO4, 1.67 mM Sodium ascorbate
PH: 7
Temperature: 30
uniprot:P17289 [tyrosine 3-monooxygenase(Enzyme) wildtype]

O2 + L_Tyrosine + Tetrahydrobiopterin -> L_Dopa + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.33 mM Tris-HCl, 15 mM dithiothreitol
PH: 7
Temperature: 37
uniprot:P17289 [tyrosine 3-monooxygenase(Enzyme) wildtype]

O2 + L_Tyrosine + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Dopa		 (( (Vmax * A) ) / (Km + A)) buffer: 0.33 mM Tris-HCl, 15 mM dithiothreitol
PH: 7
Temperature: 37
uniprot:P17289 [tyrosine 3-monooxygenase(Enzyme) wildtype]

O2 + Tetrahydrobiopterin + L_Tyrosine -> L_Dopa + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.33 mM Tris-HCl, 15 mM dithiothreitol
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY66-301 catecholamine biosynthesis
BioCyc:HUMAN_PWY66-301 catecholamine biosynthesis
Reactome:R-DME-209776 Metabolism of amine-derived hormones
Reactome:R-HSA-209905 Catecholamine biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-209905 Catecholamine biosynthesis
Reactome:R-RNO-209905 Catecholamine biosynthesis
Reactome:R-CEL-209905 Catecholamine biosynthesis
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-HSA-209776 Metabolism of amine-derived hormones
Reactome:R-MMU-209776 Metabolism of amine-derived hormones
Reactome:R-RNO-209776 Metabolism of amine-derived hormones
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-DME-209905 Catecholamine biosynthesis
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-209776 Metabolism of amine-derived hormones
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4220 Neurotransmitter disorders
WikiPathways:WP5376 17p13.3 (YWHAE) copy number variation
WikiPathways:WP4156 Biosynthesis and regeneration of tetrahydrobiopterin and catabolism of phenylalanine
PathBank:SMP0000497 Tyrosine Hydroxylase Deficiency
PathBank:SMP0000012 Catecholamine Biosynthesis
PathBank:SMP0000170 Aromatic L-Aminoacid Decarboxylase Deficiency