EC: 1.14.13.9

kynurenine 3-monooxygenase (L-kynurenine,NADPH:oxygen oxidoreductase (3-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:O15229 [FAD]

O2 + H+ + NADPH + L_Kynurenine -> 3_Hydroxy_L_kynurenine + H2O + NADP+		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (( (( (( (K1 * K2) ) + (K2 * A)) ) + (K1 * B)) ) + (( (( (I * K1) ) * K2) ) / Ki)) ) + (A * B)) ) + (( (( (K2 * A) ) * I) ) / (alpha * Ki))) ) + (( (( (K1 * B) ) * I) ) / (beta * Ki))) ) + (( (( (A * B) ) * I) ) / (( (alpha * beta) ) * Ki)))) buffer: 0.05 M potassium phosphate, 2 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:O88867 [kynurenine 3-monooxygenase(Enzyme) wildtype]

L_Kynurenine + H+ + NADPH + O2 -> NADP+ + H2O + 3_Hydroxy_L_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris/Cl, 10 mM EDTA, 100 mM KCl, 3 mM Glucose 6-phosphate, 0.01 U/ml glucose-6-phosphate dehydrogenase
PH: 8.1
Temperature: 37
uniprot:O88867 [kynurenine 3-monooxygenase(Enzyme) wildtype]

O2 + L_Kynurenine + NADH + H+ -> H2O + 3_Hydroxy_L_kynurenine + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris/Cl, 10 mM EDTA, 100 mM KCl, 3 mM Glucose 6-phosphate, 0.01 U/ml glucose-6-phosphate dehydrogenase
PH: 8.1
Temperature: 37
uniprot:Q11PP7 [FAD]

L_Kynurenine + O2 + H+ + NADPH -> H2O + NADP+ + 3_Hydroxy_L_kynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M Tris-acetate, 1 mM DTT, 0.2 M mannitol, 0.2% Triton X-100, 0.01 M KCl
PH: 8
Temperature: 36
uniprot:Q11PP7 [FAD]

H+ + NADPH + O2 + L_Kynurenine -> 3_Hydroxy_L_kynurenine + NADP+ + H2O		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 0.05 M Tris-acetate, 1 mM DTT, 0.2 M mannitol, 0.2% Triton X-100, 0.01 M KCl
PH: 8
Temperature: 36
uniprot:Q84HF5 [FAD]

O2 + H+ + NADPH + L_Kynurenine -> H2O + NADP+ + 3_Hydroxy_L_kynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 2 mM DTT
PH: 7.5
Temperature: 5
uniprot:Q84HF5 [FAD]

H+ + NADPH + O2 + L_Kynurenine -> NADP+ + 3_Hydroxy_L_kynurenine + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 2 mM DTT
PH: 7.5
Temperature: 25
uniprot:Q84HF5 [FAD]

NADPH + H+ + O2 + L_Kynurenine -> 3_Hydroxy_L_kynurenine + H2O + NADP+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 2 mM DTT
PH: 7.5
Temperature: 5
uniprot:Q84HF5 [FAD]

H+ + NADPH + L_Kynurenine + O2 -> NADP+ + H2O + 3_Hydroxy_L_kynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 2 mM DTT
PH: 7.5
Temperature: 5
uniprot:Q84HF5 [FAD]

H+ + O2 + NADPH + L_Kynurenine -> NADP+ + 3_Hydroxy_L_kynurenine + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 2 mM DTT
PH: 7.5
Temperature: 25
uniprot:Q84HF5 [FAD]

H+ + NADPH + O2 + L_Kynurenine -> NADP+ + 3_Hydroxy_L_kynurenine + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 2 mM DTT
PH: 7.5
Temperature: 25
uniprot:O15229 [FAD]

O2 + NADPH + L_Kynurenine + H+ -> H2O + NADP+ + 3_Hydroxy_L_kynurenine		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 2 mM DTT, 1 mM EDTA, 0.1 mM Chaps, 3 mM D-glucose-6-phosphate, 1 U/ml glucose- 6-phosphate dehydrogenase
PH: 7.5
Temperature: 20
uniprot:O15229 [n-Dodecyl-beta-D-maltoside]

NADPH + O2 + H+ + L_Kynurenine -> H2O + 3_Hydroxy_L_kynurenine + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 2 mM DTT, 1 mM EDTA, 0.1 mM CHAPS
PH: 7.5
Temperature: 20
uniprot:O15229 [n-Dodecyl-beta-D-maltoside]

L_Kynurenine + O2 + NADPH + H+ -> 3_Hydroxy_L_kynurenine + H2O + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 2 mM DTT, 1 mM EDTA, 0.1 mM CHAPS
PH: 7.5
Temperature: 20
uniprot:O15229 [kynurenine 3-monooxygenase(Enzyme) wildtype N-terminal GST-fusion]

L_Kynurenine + H+ + NADPH + O2 -> NADP+ + 3_Hydroxy_L_kynurenine + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 2 mM DTT, 1 mM EDTA, 0.1 mM CHAPS
PH: 7.5
Temperature: 20
uniprot:O15229 [kynurenine 3-monooxygenase(Enzyme) wildtype N-terminal GST-fusion]

H+ + O2 + NADPH + L_Kynurenine -> H2O + 3_Hydroxy_L_kynurenine + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 2 mM DTT, 1 mM EDTA, 0.1 mM CHAPS
PH: 7.5
Temperature: 20
uniprot:O15229 [FAD]

H+ + NADPH + L_Kynurenine + O2 -> 3_Hydroxy_L_kynurenine + H2O + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES, 4 mM MgCl2, 2 mM Glucose-6 phosphate, 10 U/ml Glucose-6 phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:O15229 [FAD]

O2 + L_Kynurenine + H+ + NADPH -> NADP+ + 3_Hydroxy_L_kynurenine + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES, 4 mM MgCl2, 2 mM Glucose-6 phosphate, 10 U/ml Glucose-6 phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:O15229 [FAD]

L_Kynurenine + O2 + NADPH + H+ -> H2O + 3_Hydroxy_L_kynurenine + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES, 4 mM MgCl2, 2 mM Glucose-6 phosphate, 10 U/ml Glucose-6 phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:O15229 [FAD]

NADPH + O2 + L_Kynurenine + H+ -> H2O + NADP+ + 3_Hydroxy_L_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES, 4 mM MgCl2, 2 mM Glucose-6 phosphate, 10 U/ml Glucose-6 phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:O15229 [FAD]

NADPH + L_Kynurenine + H+ + O2 -> H2O + 3_Hydroxy_L_kynurenine + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 2 mM DTT, 1 mM EDTA, 0.1 mM Chaps
PH: 7.5
Temperature: 20
uniprot:Q84HF5 [FAD]

L_Kynurenine + O2 + NADPH + H+ -> H2O + NADP+ + 3_Hydroxy_L_kynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 2 mM DTT, 1 mM EDTA, 0.1 mM Chaps
PH: 7.5
Temperature: 20

Pathways

pathway id name
BioCyc:HUMAN_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:META_PWY-3502 superpathway of NAD biosynthesis in eukaryotes
BioCyc:META_PWY-5651 L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:YEAST_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_NADSYN-PWY NAD biosynthesis II (from tryptophan)
BioCyc:HUMAN_PWY-6309 L-kynurenine degradation
BioCyc:META_NADSYN-PWY NAD de novo biosynthesis II (from tryptophan)
BioCyc:YEAST_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:META_TRYPTOPHAN-DEGRADATION-1 L-tryptophan degradation III (eukaryotic)
BioCyc:META_PWY-6309 L-tryptophan degradation XI (mammalian, via kynurenine)
BioCyc:MOUSE_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation III (eukaryotic)
BioCyc:MOUSE_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71240 Tryptophan catabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71240 Tryptophan catabolism
Reactome:R-MMU-71240 Tryptophan catabolism
Reactome:R-RNO-71240 Tryptophan catabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-XTR-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DDI-71240 Tryptophan catabolism
Reactome:R-DME-71240 Tryptophan catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-71240 Tryptophan catabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DRE-71240 Tryptophan catabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-71240 Tryptophan catabolism
WikiPathways:WP5044 Kynurenine pathway and links to cell senescence
PathBank:SMP0002353 Tryptophan Metabolism
PathBank:SMP0087330 Tryptophan Metabolism
PathBank:SMP0000063 Tryptophan Metabolism
PathBank:SMP0002338 NAD Metabolism
PathBank:SMP0087451 Tryptophan Metabolism