oleate β-oxidation (reductase-dependent)

Ntamack AG, Karpichev IV, Gould SJ, Small GM, Schulz H. Oleate ß-oxidation in yeast involves thioesterase but not Yor180c protein that is not a dienoyl-CoA isomerase. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2009 May;1791(5):371–8. doi: 10.1016/j.bbalip.2009.01.026.; Hiltunen JK, Mursula AM, Rottensteiner H, Wierenga RK, Kastaniotis AJ, Gurvitz A. The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev. 2003 Apr;27(1):35–64. doi: 10.1016/s0168-6445(03)00017-2. PMID: 12697341.; Gurvitz A, Mursula AM, Yagi AI, Hartig A, Ruis H, Rottensteiner H, Hiltunen JK. Alternatives to the isomerase-dependent pathway for the beta-oxidation of oleic acid are dispensable in Saccharomyces cerevisiae. Identification of YOR180c/DCI1 encoding peroxisomal delta(3,5)-delta(2,4)-dienoyl-CoA isomerase. J Biol Chem. 1999 Aug 27;274(35):24514–21. doi: 10.1074/jbc.274.35.24514. PMID: 10455114.; Geisbrecht BV, Schulz K, Nau K, Geraghty MT, Schulz H, Erdmann R, Gould SJ. Preliminary Characterization of Yor180Cp: Identification of a Novel Peroxisomal Protein of Saccharomyces cerevisiae Involved in Fatty Acid Metabolism. Biochemical and Biophysical Research Communications. 1999 Jun;260(1):28–34. doi: 10.1006/bbrc.1999.0860.; Novikov DK, Koivuranta KT, Helander HM, Filppula SA, Yagi AI, Qin YM, Hiltunen KJ. Enzymology of beta-oxidation of (poly)unsaturated fatty acids. Adv Exp Med Biol. 1999;466():301–9. doi: 10.1007/0-306-46818-2_34. PMID: 10709656.; Filppula SA, Yagi AI, Kilpeläinen SH, Novikov D, FitzPatrick DR, Vihinen M, Valle D, Hiltunen JK. Delta3,5-delta2,4-dienoyl-CoA isomerase from rat liver. Molecular characterization. J Biol Chem. 1998 Jan 02;273(1):349–55. doi: 10.1074/jbc.273.1.349. PMID: 9417087.; Gurvitz A, Rottensteiner H, Kilpeläinen SH, Hartig A, Hiltunen JK, Binder M, Dawes IW, Hamilton B. The Saccharomyces cerevisiae Peroxisomal 2,4-Dienoyl-CoA Reductase Is Encoded by the Oleate-inducible GeneSPS19. Journal of Biological Chemistry. 1997 Aug;272(35):22140–7. doi: 10.1074/jbc.272.35.22140.; Luo MJ, Smeland TE, Shoukry K, Schulz H. Delta 3,5, delta 2,4-dienoyl-CoA isomerase from rat liver mitochondria. Purification and characterization of a new enzyme involved in the beta-oxidation of unsaturated fatty acids. Journal of Biological Chemistry. 1994 Jan;269(4):2384–8. doi: 10.1016/s0021-9258(17)41957-0.; Coe JGS, Murray LE, Kennedy CJ, Dawes LW. Isolation and characterization of sporulation-specific promoters in the yeast Saccharomyces cerevisiae. Molecular Microbiology. 1992 Jan;6(1):75–81. doi: 10.1111/j.1365-2958.1992.tb00839.x.

Metabolites

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.3.1.124 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing] (3E)-3-enoyl-CoA:NADP+ 4-oxidoreductase
5.3.3.21 DELTA3,5-DELTA2,4-dienoyl-CoA isomerase (3E,5Z)-alka-3,5-dienoyl-CoA DELTA3,5-DELTA2,4 isomerase
5.3.3.8 DELTA3-DELTA2-enoyl-CoA isomerase (3Z/3E)-alk-3-enoyl-CoA (2E)-isomerase


Proteins

Protein ID name full name
P32573 SPS19 Peroxisomal 2,4-dienoyl-CoA reductase SPS19 [(3E)-enoyl-CoA-producing]
Q05871 ECI1 3,2-trans-enoyl-CoA isomerase
Q08558 DCI1 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase