plant sterol biosynthesis II

Arnqvist L, Dutta PC, Jonsson L, Sitbon F. Reduction of cholesterol and glycoalkaloid levels in transgenic potato plants by overexpression of a type 1 sterol methyltransferase cDNA. Plant Physiol. 2003 Apr;131(4):1792–9. PMID: 12692338; PMCID: PMC166935.; Schrick K, Mayer U, Martin G, Bellini C, Kuhnt C, Schmidt J, Jürgens G. Interactions between sterol biosynthesis genes in embryonic development of Arabidopsis. The Plant Journal. 2002 Jul;31(1):61–73. doi: 10.1046/j.1365-313x.2002.01333.x.; Nomura T, Kitasaka Y, Takatsuto S, Reid JB, Fukami M, Yokota T. Brassinosteroid/Sterol Synthesis and Plant Growth as Affected bylka and lkb Mutations of Pea1. 1999 Apr 01;119(4):1517–26. doi: 10.1104/pp.119.4.1517.; Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier CP, Gregory BD, Ross AS, Tanaka A, Yoshida S, Tax FE, Feldmann KA. The Arabidopsis dwf7/ste1 Mutant Is Defective in the ?7 Sterol C-5 Desaturation Step Leading to Brassinosteroid Biosynthesis. Plant Cell. 1999 Feb;11(2):207–21. doi: 10.1105/tpc.11.2.207.

Metabolites

(S)-2,3-Epoxysqualene

Formula: C30H50O (426.386145)

CAS ID: 54910-48-4

7-Dehydrodesmosterol

Formula: C27H42O (382.3235482)

CAS ID: 1715-86-2

Cholesterol

Formula: C27H46O (386.3548466)

CAS ID: 57-88-5

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Cycloartenol

Formula: C30H50O (426.386145)

CAS ID: 469-38-5

Desmosterol

Formula: C27H44O (384.3391974)

CAS ID: 313-04-2

Formate

Formula: CH2O2 (46.0054792)

CAS ID: 64-18-6

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Oxygen

Formula: O2 (31.98983)

CAS ID: 7782-44-7

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.1.1.418 plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating) 3beta-hydroxysteroid-4alpha-carboxylate:NAD+ 3-oxidoreductase (decarboxylating)
1.14.14.17 squalene monooxygenase squalene,NADPH-hemoprotein:oxygen oxidoreductase (2,3-epoxidizing
1.14.18.10 plant 4,4-dimethylsterol C-4alpha-methyl-monooxygenase 24-methylidenecycloartanol,ferrocytochrome-b5:oxygen oxidoreductase (C-4alpha-methyl-hydroxylating)
1.14.18.11 plant 4alpha-monomethylsterol monooxygenase 24-ethylidenelophenol,ferrocytochrome-b5:oxygen oxidoreductase (C-4alpha-methyl-hydroxylating)
1.14.18.9 4alpha-methylsterol monooxygenase 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol,ferrocytochrome-b5:oxygen oxidoreductase (C4alpha-methyl-hydroxylating)
5.4.99.8 cycloartenol synthase (S)-2,3-Epoxysqualene mutase (cyclizing, cycloartenol-forming)


Proteins

Protein ID name full name