3,8-divinyl-chlorophyllide a biosynthesis III (aerobic, light independent)

Meinecke L, Alawady A, Schroda M, Willows R, Kobayashi MC, Niyogi KK, Grimm B, Beck CF. Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX. Plant Molecular Biology. 2010 Feb 03;72(6):643–58. doi: 10.1007/s11103-010-9604-9.; Hill KL, Merchant S. Coordinate expression of coproporphyrinogen oxidase and cytochrome c6 in the green alga Chlamydomonas reinhardtii in response to changes in copper availability. The EMBO Journal. 1995 Mar;14(5):857–65. doi: 10.1002/j.1460-2075.1995.tb07067.x.; Li J, Goldschmidt-Clermont M, Timko MP. Chloroplast-encoded chlB is required for light-independent protochlorophyllide reductase activity in Chlamydomonas reinhardtii. Plant Cell. 1993 Dec;5(12):1817–29. PMID: 8305874; PMCID: PMC160407.; Liu XQ, Xu H, Huang C. Chloroplast chlB gene is required for light-independent chlorophyll accumulation in Chlamydomonas reinhardtii. Plant Mol Biol. 1993 Oct;23(2):297–308. doi: 10.1007/bf00029006. PMID: 8219066.; Suzuki JY, Bauer CE. Light-independent chlorophyll biosynthesis: involvement of the chloroplast gene chlL (frxC). Plant Cell. 1992 Aug;4(8):929–40. PMID: 1392602; PMCID: PMC160185.; Walker CJ, Mansfield KE, Rezzano IN, Hanamoto CM, Smith KM, Castelfranco PA. The magnesium-protoporphyrin IX (oxidative) cyclase system. Studies on the mechanism and specificity of the reaction sequence. Biochem J. 1988 Oct 15;255(2):685–92. PMID: 3202840; PMCID: PMC1135280.

Metabolites

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Hydrogen peroxide

Formula: H2O2 (34.0054792)

CAS ID: 7722-84-1

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Oxygen

Formula: O2 (31.98983)

CAS ID: 7782-44-7

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.3.99.-
1.14.13.81 magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase magnesium-protoporphyrin-IX 13-monomethyl ester,NADPH:oxygen oxidoreductase (hydroxylating)
1.3.7.7 ferredoxin:protochlorophyllide reductase (ATP-dependent) ATP-dependent ferredoxin:protochlorophyllide-a 7,8-oxidoreductase


Proteins

Protein ID name full name
P0DJN9 acsF Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
P72584 acsF1 Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 1
P72793 hemJ Protoporphyrinogen IX oxidase
P74134 acsF2 Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2
P95463 chlB Light-independent protochlorophyllide reductase subunit B
Q00237 chlL Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein
Q04607 chlN Light-independent protochlorophyllide reductase subunit N