phospholipid remodeling (phosphatidate, yeast)

Henry SA, Kohlwein SD, Carman GM. Metabolism and regulation of glycerolipids in the yeast Saccharomyces cerevisiae. Genetics. 2012 Feb;190(2):317–49. PMID: 22345606; PMCID: PMC3276621.; Ayciriex S, Le Guédard M, Camougrand N, Velours G, Schoene M, Leone S, Wattelet-Boyer V, Dupuy JW, Shevchenko A, Schmitter JM, Lessire R, Bessoule JJ, Testet E. YPR139c/LOA1 encodes a novel lysophosphatidic acid acyltransferase associated with lipid droplets and involved in TAG homeostasis. Mol Biol Cell. 2012 Jan;23(2):233–46. PMID: 22090344; PMCID: PMC3258169.; Rajakumari S, Daum G. Multiple Functions as Lipase, Steryl Ester Hydrolase, Phospholipase, and Acyltransferase of Tgl4p from the Yeast Saccharomyces cerevisiae. Journal of Biological Chemistry. 2010 May;285(21):15769–76. doi: 10.1074/jbc.m109.076331.; Rajakumari S, Daum G. Janus-faced Enzymes Yeast Tgl3p and Tgl5p Catalyze Lipase and Acyltransferase Reactions. MBoC. 2010 Feb 15;21(4):501–10. doi: 10.1091/mbc.e09-09-0775.; Petschnigg J, Wolinski H, Kolb D, Zellnig G, Kurat CF, Natter K, Kohlwein SD. Good Fat, Essential Cellular Requirements for Triacylglycerol Synthesis to Maintain Membrane Homeostasis in Yeast. Journal of Biological Chemistry. 2009 Nov;284(45):30981–93. doi: 10.1074/jbc.m109.024752.; Jain S, Stanford N, Bhagwat N, Seiler B, Costanzo M, Boone C, Oelkers P. Identification of a Novel Lysophospholipid Acyltransferase in Saccharomyces cerevisiae. Journal of Biological Chemistry. 2007 Oct;282(42):30562–9. doi: 10.1074/jbc.m706326200.; Boumann HA, Gubbens J, Koorengevel MC, Oh CS, Martin CE, Heck AJ, Patton-Vogt J, Henry SA, de Kruijff B, de Kroon AI. Depletion of phosphatidylcholine in yeast induces shortening and increased saturation of the lipid acyl chains: evidence for regulation of intrinsic membrane curvature in a eukaryote. Mol Biol Cell. 2006 Feb;17(2):1006–17. PMID: 16339082; PMCID: PMC1356607.; Kurat CF, Natter K, Petschnigg J, Wolinski H, Scheuringer K, Scholz H, Zimmermann R, Leber R, Zechner R, Kohlwein SD. Obese Yeast: Triglyceride Lipolysis Is Functionally Conserved from Mammals to Yeast. Journal of Biological Chemistry. 2006 Jan;281(1):491–500. doi: 10.1074/jbc.m508414200.; Athenstaedt K, Daum G. Tgl4p and Tgl5p, Two Triacylglycerol Lipases of the Yeast Saccharomyces cerevisiae Are Localized to Lipid Particles. Journal of Biological Chemistry. 2005 Nov;280(45):37301–9. doi: 10.1074/jbc.m507261200.; Wagner S, Paltauf F. Generation of glycerophospholipid molecular species in the yeast Saccharomyces cerevisiae. Fatty acid pattern of phospholipid classes and selective acyl turnover at sn-1 and sn-2 positions. Yeast. 1994 Nov;10(11):1429–37. doi: 10.1002/yea.320101106.; Nagiec MM, Wells GB, Lester RL, Dickson RC. A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase. Journal of Biological Chemistry. 1993 Oct;268(29):22156–63. doi: 10.1016/s0021-9258(20)80661-9.; Brown P. The Board of Nursing explains guidelines, rules and advisory opinions. Wash Nurse. 1991 Nov;21(8):15–6. PMID: 1767529.; Cullis PR, De Kruijff B. The polymorphic phase behaviour of phosphatidylethanolamines of natural and synthetic origin. A 31P NMR study. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1978 Oct;513(1):31–42. doi: 10.1016/0005-2736(78)90109-8.; LANDS WE. LIPID METABOLISM. Annu Rev Biochem. 1965;34():313–46. doi: 10.1146/annurev.bi.34.070165.001525. PMID: 14321173.; LANDS WE. Metabolism of glycerolipids. 2. The enzymatic acylation of lysolecithin. J Biol Chem. 1960 Aug;235():2233–7. PMID: 14413818.

Metabolites

Copper

Formula: Cu (62.929599)

CAS ID: 7440-50-8

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

Triton X-100

Formula: C16H26O2 (250.1932696)

CAS ID: 2315-67-5

oleate

Formula: C18H33O2 (281.2480418)

CAS ID: 115-06-0



Enzyme

EC Number name full name note
2.3.1.n6
2.3.1.23 1-acylglycerophosphocholine O-acyltransferase acyl-CoA:1-acyl-sn-glycero-3-phosphocholine O-acyltransferase
2.3.1.51 1-acylglycerol-3-phosphate O-acyltransferase acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase
3.1.1.3 triacylglycerol lipase triacylglycerol acylhydrolase


Proteins

Protein ID name full name
P33333 SLC1 1-acyl-sn-glycerol-3-phosphate acyltransferase
P36165 TGL4 Triacylglycerol lipase 4
Q06508 LOA1 Lysophosphatidic acid:oleoyl-CoA acyltransferase 1
Q08548 ALE1 Lysophospholipid acyltransferase
Q12043 TGL5 Triacylglycerol lipase 5