mAGP
Grzegorzewicz AE, Ma Y, Jones V, Crick D, Liav A, McNeil MR. Development of a microtitre plate-based assay for lipid-linked glycosyltransferase products using the mycobacterial cell wall rhamnosyltransferase WbbL. 2008 Dec 01;154(12):3724–30. doi: 10.1099/mic.0.2008/023366-0.; Birch HL, Alderwick LJ, Bhatt A, Rittmann D, Krumbach K, Singh A, Bai Y, Lowary TL, Eggeling L, Besra GS. Biosynthesis of mycobacterial arabinogalactan: identification of a novel a(1?3) arabinofuranosyltransferase. Molecular Microbiology. 2008 Aug 05;69(5):1191–206. doi: 10.1111/j.1365-2958.2008.06354.x.; Belánová M, Dianisková P, Brennan PJ, Completo GC, Rose NL, Lowary TL, Mikusová K. Galactosyl transferases in mycobacterial cell wall synthesis. J Bacteriol. 2008 Feb;190(3):1141–5. PMID: 18055597; PMCID: PMC2223555.; Mikus?ova´ K, Bela´n?ova´ M, Kordula´kova´ J, Honda K, McNeil MR, Mahapatra S, Crick DC, Brennan PJ. Identification of a Novel Galactosyl Transferase Involved in Biosynthesis of the Mycobacterial Cell Wall. J Bacteriol. 2006 Sep 15;188(18):6592–8. doi: 10.1128/jb.00489-06.; Mills JA, Motichka K, Jucker M, Wu HP, Uhlik BC, Stern RJ, Scherman MS, Vissa VD, Pan F, Kundu M, Ma YF, McNeil M. Inactivation of the Mycobacterial Rhamnosyltransferase, Which Is Needed for the Formation of the Arabinogalactan-Peptidoglycan Linker, Leads to Irreversible Loss of Viability. Journal of Biological Chemistry. 2004 Oct;279(42):43540–6. doi: 10.1074/jbc.m407782200.; Crick DC, Mahapatra S, Brennan PJ. Biosynthesis of the arabinogalactan-peptidoglycan complex of Mycobacterium tuberculosis. Glycobiology. 2001 Sep;11(9):107R–118R. doi: 10.1093/glycob/11.9.107r. PMID: 11555614.
Metabolites
Enzyme
| EC Number | name | full name | note |
|---|---|---|---|
| 2.4.2.- | |||
| 1.1.1.333 | decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose 2-reductase | trans,octacis-decaprenylphospho-beta-D-arabinofuranose:NAD+ 2-oxidoreductase | |
| 1.1.98.3 | decaprenylphospho-beta-D-ribofuranose 2-dehydrogenase | trans,octacis-decaprenylphospho-beta-D-ribofuranose:FAD 2-oxidoreductase | |
| 2.3.1.122 | trehalose O-mycolyltransferase | alpha,alpha-trehalose-6-mycolate:alpha,alpha-trehalose-6-mycolate 6'-mycolyltransferase | |
| 2.3.1.20 | diacylglycerol O-acyltransferase | acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase | |
| 2.4.1.287 | rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,4/1,5-galactofuranosyltransferase | UDP-alpha-D-galactofuranose:alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/4-beta-galactofuranosyltransferase (configuration-inverting) | |
| 2.4.1.288 | galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase | UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase | |
| 2.4.1.289 | N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase | dTDP-6-deoxy-beta-L-mannose:N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 3-alpha-L-rhamnosyltransferase | |
| 2.4.2.45 | decaprenyl-phosphate phosphoribosyltransferase | trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate:diphosphate phospho-alpha-D-ribosyltransferase | |
| 2.4.2.46 | galactan 5-O-arabinofuranosyltransferase | galactofuranan:trans,octacis-decaprenylphospho-beta-D-arabinofuranose 5-O-alpha-D-arabinofuranosyltransferase | |
| 2.4.2.47 | arabinofuranan 3-O-arabinosyltransferase | alpha-(1->5)-arabinofuranan:trans,octacis-decaprenylphospho-beta-D-arabinofuranose 3-O-alpha-D-arabinofuranosyltransferase | |
| 2.7.8.35 | UDP-N-acetylglucosamine-decaprenyl-phosphate N-acetylglucosaminephosphotransferase | UDP-N-acetyl-alpha-D-glucosamine:trans,octacis-decaprenyl-phosphate N-acetylglucosaminephosphotransferase | |
| 3.1.3.111 | decaprenylphosphoryl-5-phosphoribose phosphatase | trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate phosphohydrolase | |
| 5.1.3.2 | UDP-glucose 4-epimerase | UDP-glucose 4-epimerase | |
| 5.4.99.9 | UDP-galactopyranose mutase | UDP-D-galactopyranose furanomutase |
Proteins
| Protein ID | name | full name |
|---|---|---|
| O53582 | aftB | Possible arabinofuranosyltransferase AftB |
| O53585 | glfT2 | Bifunctional UDP-galactofuranosyl transferase GlfT2 |
| P0A560 | embA | Probable arabinosyltransferase A |
| P9WFR5 | ubiA | Decaprenylphosphoryl-5-phosphoribose (DPPR) synthase (decaprenyl-phosphate 5-phosphoribosyltransferase) |
| P9WGS9 | dprE2 | Decaprenylphosphoryl-2-keto-beta-D-erythro-pentose reductase |
| P9WI53 | Rv3807c | Possible conserved transmembrane protein |
| P9WIQ1 | glf | UDP-galactopyranose mutase Glf (UDP-GALP mutase) (NAD+-flavin adenine dinucleotide-requiring enzyme) |
| P9WJF1 | dprE1 | Decaprenylphosphoryl-beta-D-ribose 2'-oxidase |
| P9WMW5 | wecA | Decaprenyl-phosphate N-acetylglucosaminephosphotransferase |
| P9WMX3 | glfT1 | UDP-galactofuranosyl transferase GlfT1 |
| P9WMY3 | wbbL1 | dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol,a-3-L-rhamnosyl transferase WbbL1 (alpha-L-rhamnose-(1->3)-alpha-D-GlcNAc(1->P)-P- decaprenyl) |
| P9WMZ7 | aftC | Possible arabinofuranosyltransferase AftC |
| P9WN03 | aftA | Arabinofuranosyltransferase AftA |
| P9WN67 | galE1 | UDP-glucose 4-epimerase GalE1 (galactowaldenase) (UDP-galactose 4-epimerase) (uridine diphosphate galactose 4-epimerase) (uridine diphospho-galactose 4-epimerase) |
| P9WNL7 | embB | Probable arabinosyltransferase B |
| P9WQN9 | fbpC | Diacylglycerol acyltransferase/mycolyltransferase Ag85C |
| P9WQP1 | fbpB | Diacylglycerol acyltransferase/mycolyltransferase Ag85B |
| P9WQP3 | fbpA | Diacylglycerol acyltransferase/mycolyltransferase Ag85A |