decaprenoxanthin diglucoside biosynthesis

Heider SAE, Peters-Wendisch P, Netzer R, Stafnes M, Brautaset T, Wendisch VF. Production and glucosylation of C50 and C40 carotenoids by metabolically engineered Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 2013 Nov 24;98(3):1223–35. doi: 10.1007/s00253-013-5359-y.; Heider SAE, Peters-Wendisch P, Wendisch VF. Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum. BMC Microbiology. 2012 Sep 10;12(1):198. doi: 10.1186/1471-2180-12-198.; Netzer R, Stafsnes MH, Andreassen T, Goksøyr A, Bruheim P, Brautaset T. Biosynthetic Pathway for ?-Cyclic Sarcinaxanthin in Micrococcus luteus : Heterologous Expression and Evidence for Diverse and Multiple Catalytic Functions of C 50 Carotenoid Cyclases. J Bacteriol. 2010 Nov;192(21):5688–99. doi: 10.1128/jb.00724-10.; Krubasik P, Takaichi S, Maoka T, Kobayashi M, Masamoto K, Sandmann G. Detailed biosynthetic pathway to decaprenoxanthin diglucoside in Corynebacterium glutamicum and identification of novel intermediates. Arch Microbiol. 2001 Sep;176(3):217–23. doi: 10.1007/s002030100315. PMID: 11511870.; Krubasik P, Kobayashi M, Sandmann G. Expression and functional analysis of a gene cluster involved in the synthesis of decaprenoxanthin reveals the mechanisms for C50 carotenoid formation. European Journal of Biochemistry. 2001 Jul;268(13):3702–8. doi: 10.1046/j.1432-1327.2001.02275.x.

Metabolites

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

Flavuxanthin

Formula: C50H72O2 (704.5532012)

CAS ID: 360063-98-5

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Lycopene

Formula: C40H56 (536.4381776)

CAS ID: 502-65-8

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0



Enzyme

EC Number name full name note
2.5.1.149 lycopene elongase/hydratase (flavuxanthin-forming) dimethylallyl-diphosphate:all-trans-lycopene dimethylallyltransferase (hydrating, flavuxanthin-forming)


Proteins

Protein ID name full name
Q93QX2 crtEb Lycopene elongase/hydratase