EC: 6.3.5.12

Ni-sirohydrochlorin a,c-diamide synthase (Ni-sirohydrochlorin:L-glutamine amido-ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q46FL0 [Mg2+]

2 ATP + Nickel_sirohydrochlorin + 2 H2O + 2 L_Glutamine -> 2 ADP + 2 H+ + 2 L_Glutamate + 2 Phosphate + Nickel_sirohydrochlorin_a,c_diamide		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 20 mM Tris-HCl, 100 mM NaCl
PH: 8
Temperature: 20
uniprot:Q46FL0 [Mg2+]

2 L_Glutamine + Nickel_sirohydrochlorin + 2 H2O + 2 ATP -> 2 ADP + 2 L_Glutamate + 2 H+ + 2 Phosphate + Nickel_sirohydrochlorin_a,c_diamide		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM Tris-HCl, 100 mM NaCl
PH: 8
Temperature: 20

Pathways

pathway id name
BioCyc:META_PWY-5196 factor 430 biosynthesis