EC: 6.3.4.12

glutamate-methylamine ligase (L-glutamate:methylamine ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:A9ZPH9 [Mg2+]

L_Glutamate + ATP + NH3 -> ADP + Phosphate + L_Glutamine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM imidazole
PH: 7.75
Temperature: 30
uniprot:A9ZPH9 [Mg2+]

L_Glutamate + Methylamine + ATP -> N5_Methyl_L_glutamine + Phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM imidazole
PH: 7.75
Temperature: 30
uniprot:A9ZPH9 [Mg2+]

ATP + L_Glutamate + Hydroxylamine -> ADP + Phosphate + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM imidazole
PH: 8
Temperature: 30
uniprot:A9ZPH9 [Mg2+]

ATP + Methylamine + L_Glutamate -> Phosphate + N5_Methyl_L_glutamine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM imidazole
PH: 7.75
Temperature: 30
uniprot:A9ZPH9 [Mg2+]

Methylamine + L_Glutamate + ATP -> Phosphate + N5_Methyl_L_glutamine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM imidazole
PH: 7.75
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6962 superpathway of trimethylamine degradation
BioCyc:META_PWY-6965 methylamine degradation II