EC: 6.3.3.3

dethiobiotin synthase (7,8-diaminononanoate:carbon-dioxide cyclo-ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P13000 [dethiobiotin synthase(Enzyme) wildtype]

CO2 + 7,8_Diaminononanoate + ATP -> Phosphate + Dethiobiotin + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant E12A]

7,8_Diaminononanoate + ATP + CO2 -> Dethiobiotin + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant E12D]

CO2 + ATP + 7,8_Diaminononanoate -> Dethiobiotin + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant T11V]

7,8_Diaminononanoate + CO2 + ATP -> ADP + Phosphate + Dethiobiotin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant K37L]

CO2 + ATP + 7,8_Diaminononanoate -> Dethiobiotin + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant K37Q]

ATP + CO2 + 7,8_Diaminononanoate -> Dethiobiotin + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant K15Q]

CO2 + ATP + 7,8_Diaminononanoate -> Phosphate + Dethiobiotin + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant K37R]

7,8_Diaminononanoate + CO2 + ATP -> Dethiobiotin + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant S41C]

7,8_Diaminononanoate + CO2 + ATP -> Dethiobiotin + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant S41A]

ATP + 7,8_Diaminononanoate + CO2 -> Phosphate + Dethiobiotin + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant E12A]

CO2 + 7,8_Diaminononanoate + ATP -> Phosphate + ADP + Dethiobiotin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) wildtype]

ATP + CO2 + 7,8_Diaminononanoate -> ADP + Phosphate + Dethiobiotin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant E12D]

ATP + 7,8_Diaminononanoate + CO2 -> ADP + Dethiobiotin + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant K15Q]

CO2 + 7,8_Diaminononanoate + ATP -> Dethiobiotin + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant T11V]

CO2 + ATP + 7,8_Diaminononanoate -> ADP + Dethiobiotin + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant K37L]

7,8_Diaminononanoate + ATP + CO2 -> Phosphate + ADP + Dethiobiotin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant S41A]

7,8_Diaminononanoate + CO2 + ATP -> ADP + Phosphate + Dethiobiotin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant S41C]

ATP + CO2 + 7,8_Diaminononanoate -> ADP + Dethiobiotin + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant K37Q]

7,8_Diaminononanoate + ATP + CO2 -> Phosphate + Dethiobiotin + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant K37R]

CO2 + ATP + 7,8_Diaminononanoate -> ADP + Phosphate + Dethiobiotin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) wildtype]

ATP + 7,8_Diaminononanoate + CO2 -> Dethiobiotin + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant E12D]

7,8_Diaminononanoate + CO2 + ATP -> Phosphate + ADP + Dethiobiotin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant E12A]

7,8_Diaminononanoate + ATP + CO2 -> ADP + Phosphate + Dethiobiotin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant S41A]

7,8_Diaminononanoate + CO2 + ATP -> Dethiobiotin + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25
uniprot:P13000 [dethiobiotin synthase(Enzyme) mutant S41C]

7,8_Diaminononanoate + ATP + CO2 -> Dethiobiotin + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:CAULONA1000_PWY-3701 biotin biosynthesis II
BioCyc:CAULONA1000_BIOTIN-SYNTHESIS-PWY biotin biosynthesis I
BioCyc:CAULO_PWY0-1507 biotin biosynthesis from 8-amino-7-oxononanoate
BioCyc:ECOL316407_PWY0-1507 biotin biosynthesis from 8-amino-7-oxononanoate I
PathBank:SMP0000785 Biotin Metabolism
PathBank:SMP0121263 Biotin Metabolism