EC: 6.2.1.12

4-coumarate-CoA ligase (4-coumarate:CoA ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

ATP + Coenzyme_A + 4_Coumarate -> AMP + Diphosphate + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/deltaV355]

4_Coumarate + ATP + Coenzyme_A -> AMP + Diphosphate + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L]

4_Coumarate + Coenzyme_A + ATP -> Diphosphate + AMP + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant deltaV355]

ATP + Coenzyme_A + 4_Coumarate -> 4_Coumaroyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/deltaL356]

ATP + Coenzyme_A + 4_Coumarate -> Diphosphate + 4_Coumaroyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

ATP + Coenzyme_A + Caffeate -> Caffeoyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L]

Caffeate + Coenzyme_A + ATP -> Caffeoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/deltaL356]

Caffeate + Coenzyme_A + ATP -> Caffeoyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/deltaV355]

Caffeate + Coenzyme_A + ATP -> Diphosphate + AMP + Caffeoyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/deltaL356]

Ferulate + ATP + Coenzyme_A -> Feruloyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/deltaV355]

Coenzyme_A + ATP + Ferulate -> Diphosphate + AMP + Feruloyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L]

Ferulate + Coenzyme_A + ATP -> Diphosphate + Feruloyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

Coenzyme_A + Ferulate + ATP -> AMP + Diphosphate + Feruloyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/deltaV355]

ATP + Sinapate + Coenzyme_A -> Sinapoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/deltaL356]

Coenzyme_A + Sinapate + ATP -> AMP + Diphosphate + Sinapoyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant deltaV355]

Caffeate + Coenzyme_A + ATP -> AMP + Caffeoyl_CoA + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

Coenzyme_A + ATP + Cinnamic_acid -> Diphosphate + AMP + Cinnamoyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320L]

ATP + Coenzyme_A + Cinnamic_acid -> Diphosphate + Cinnamoyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
- [4-coumarate---CoA ligase(Enzyme) wildtype chimeric protein At4CL2-backbone/At4CL4-SBP]

Coenzyme_A + ATP + Sinapate -> Sinapoyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9LU36 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL4]

Coenzyme_A + Sinapate + ATP -> Sinapoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293F/K320F]

Coenzyme_A + Caffeate + ATP -> Diphosphate + AMP + Caffeoyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/N256A]

Caffeate + ATP + Coenzyme_A -> Caffeoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320L]

Coenzyme_A + Caffeate + ATP -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L]

Cinnamic_acid + Coenzyme_A + ATP -> Diphosphate + AMP + Cinnamoyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/K320L/N256A]

Coenzyme_A + ATP + Cinnamic_acid -> Cinnamoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293F/K320F]

Coenzyme_A + Cinnamic_acid + ATP -> AMP + Cinnamoyl_CoA + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype His6-tagged CL2]

Coenzyme_A + ATP + 4_Coumarate -> Diphosphate + AMP + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q42524 [4-coumarate---CoA ligase(Enzyme) wildtype His6-tagged CL1]

Coenzyme_A + ATP + 4_Coumarate -> Diphosphate + 4_Coumaroyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q42524 [4-coumarate---CoA ligase(Enzyme) wildtype His6-tagged CL1]

ATP + Coenzyme_A + Ferulate -> Diphosphate + Feruloyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

4_Coumarate + ATP + Coenzyme_A -> 4_Coumaroyl_CoA + AMP + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

ATP + trans_Cinnamate + Coenzyme_A -> Cinnamoyl_CoA + AMP + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

Coenzyme_A + Caffeate + ATP -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant At4CL2 M293P/K320L]

ATP + Caffeate + Coenzyme_A -> Caffeoyl_CoA + AMP + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [Caffeate]

ATP + Triphosphate -> Adenosine_5'_tetraphosphate + Diphosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q9S725 [4-Coumarate]

ATP + Triphosphate -> Adenosine_5'_tetraphosphate + Diphosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q9S725 [Ferulate]

Triphosphate + ATP -> Adenosine_5'_tetraphosphate + Diphosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/1]

4_Coumarate + Coenzyme_A + ATP -> AMP + Diphosphate + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/11]

ATP + 4_Coumarate + Coenzyme_A -> Diphosphate + AMP + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/10]

Coenzyme_A + 4_Coumarate + ATP -> Diphosphate + 4_Coumaroyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/2]

ATP + Coenzyme_A + 4_Coumarate -> AMP + 4_Coumaroyl_CoA + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/8]

4_Coumarate + ATP + Coenzyme_A -> Diphosphate + 4_Coumaroyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/9]

ATP + Coenzyme_A + 4_Coumarate -> AMP + Diphosphate + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/7]

4_Coumarate + ATP + Coenzyme_A -> Diphosphate + 4_Coumaroyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/5]

ATP + 4_Coumarate + Coenzyme_A -> Diphosphate + AMP + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/6]

Coenzyme_A + 4_Coumarate + ATP -> 4_Coumaroyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/3]

Coenzyme_A + 4_Coumarate + ATP -> 4_Coumaroyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/4]

Coenzyme_A + ATP + 4_Coumarate -> 4_Coumaroyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/12]

ATP + 4_Coumarate + Coenzyme_A -> Diphosphate + 4_Coumaroyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/9]

Coenzyme_A + Ferulate + ATP -> Feruloyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/12]

Ferulate + Coenzyme_A + ATP -> Diphosphate + Feruloyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/11]

Ferulate + ATP + Coenzyme_A -> Diphosphate + AMP + Feruloyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/10]

ATP + Coenzyme_A + Ferulate -> AMP + Diphosphate + Feruloyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/3]

ATP + Coenzyme_A + Ferulate -> Diphosphate + AMP + Feruloyl_CoA		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/5]

ATP + Coenzyme_A + Ferulate -> Feruloyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/8]

Ferulate + Coenzyme_A + ATP -> Diphosphate + Feruloyl_CoA + AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) mutant chimeric His6-tagged CL1::2/4]

Ferulate + Coenzyme_A + ATP -> AMP + Feruloyl_CoA + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 500 mM Tris-HCl, 25 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Lys457/Ser]

Caffeate + ATP + Coenzyme_A -> Diphosphate + Caffeoyl_CoA + AMP		 (( (Vmax * S2) ) / (Km + S2)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Arg449/Gln]

Caffeate + ATP + Coenzyme_A -> Diphosphate + AMP + Caffeoyl_CoA		 (( (Vmax * S2) ) / (Km + S2)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Lys457/Ser]

ATP + Coenzyme_A + Caffeate -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S1) ) / (Km + S1)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Cys403/Ala]

ATP + Caffeate + Coenzyme_A -> Caffeoyl_CoA + Diphosphate + AMP		 (( (Vmax * S2) ) / (Km + S2)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Glu401/Gln]

Coenzyme_A + ATP + Caffeate -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S2) ) / (Km + S2)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Lys211/Ser]

Caffeate + ATP + Coenzyme_A -> Caffeoyl_CoA + Diphosphate + AMP		 (( (Vmax * S2) ) / (Km + S2)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Lys457/Ser]

Caffeate + ATP + Coenzyme_A -> AMP + Caffeoyl_CoA + Diphosphate		 (( (Vmax * S1) ) / (Km + S1)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Lys455/Thr]

ATP + Coenzyme_A + Caffeate -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S1) ) / (Km + S1)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant At4CL2 M293P/K320L]

Coenzyme_A + 4_Coumarate + ATP -> Diphosphate + AMP + 4_Coumaroyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant At4CL2 M293P/K320L]

ATP + Ferulate + Coenzyme_A -> Diphosphate + Feruloyl_CoA + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant At4CL2 M293P/K320L]

Coenzyme_A + ATP + trans_Cinnamate -> AMP + Cinnamoyl_CoA + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-Coumarate]

Triphosphate + ATP -> Adenosine_5'_tetraphosphate + Diphosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q9S725 [Caffeate]

Triphosphate + ATP -> Adenosine_5'_tetraphosphate + Diphosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q9S725 [Mg2+]

ATP + Triphosphate -> Adenosine_5'_tetraphosphate + Diphosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q9S725 [Ferulate]

Triphosphate + ATP -> Adenosine_5'_tetraphosphate + Diphosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q9S725 [Ferulate]

ATP + Triphosphate -> Adenosine_5'_tetraphosphate + Diphosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q9S725 [Ferulate]

Triphosphate + P1,P4_Bis(5'_adenosyl)_tetraphosphate -> ATP + Adenosine_5'_tetraphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES/KOH, 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype]

ATP + Caffeate + Coenzyme_A -> Diphosphate + AMP + Caffeoyl_CoA		 (( (Vmax * S1) ) / (Km + S1)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Glu401/Gln]

Coenzyme_A + ATP + Caffeate -> Diphosphate + AMP + Caffeoyl_CoA		 (( (Vmax * S1) ) / (Km + S1)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Cys403/Ala]

Caffeate + Coenzyme_A + ATP -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S1) ) / (Km + S1)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Lys211/Ser]

ATP + Caffeate + Coenzyme_A -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S1) ) / (Km + S1)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant Arg449/Gln]

Coenzyme_A + ATP + Caffeate -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S1) ) / (Km + S1)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype]

ATP + Caffeate + Coenzyme_A -> Caffeoyl_CoA + Diphosphate + AMP		 (( (Vmax * S2) ) / (Km + S2)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype]

ATP + Coenzyme_A + 3,4_Dihydroxy_trans_cinnamate -> AMP + Caffeoyl_CoA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293A]

3,4_Dihydroxy_trans_cinnamate + ATP + Coenzyme_A -> Caffeoyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P]

3,4_Dihydroxy_trans_cinnamate + Coenzyme_A + ATP -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/V294M]

Coenzyme_A + 3,4_Dihydroxy_trans_cinnamate + ATP -> Diphosphate + AMP + Caffeoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293A/V294L]

3,4_Dihydroxy_trans_cinnamate + Coenzyme_A + ATP -> Caffeoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant V294M]

Coenzyme_A + 3,4_Dihydroxy_trans_cinnamate + ATP -> Diphosphate + Caffeoyl_CoA + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant V294L]

ATP + Coenzyme_A + 3,4_Dihydroxy_trans_cinnamate -> Caffeoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293F]

ATP + Coenzyme_A + 3,4_Dihydroxy_trans_cinnamate -> Caffeoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293A]

ATP + Coenzyme_A + Ferulate -> Diphosphate + Feruloyl_CoA + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293W]

ATP + 3,4_Dihydroxy_trans_cinnamate + Coenzyme_A -> AMP + Caffeoyl_CoA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320A]

Coenzyme_A + 3,4_Dihydroxy_trans_cinnamate + ATP -> Caffeoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P/V294M]

ATP + Coenzyme_A + Ferulate -> AMP + Feruloyl_CoA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293P]

ATP + Coenzyme_A + Ferulate -> Diphosphate + AMP + Feruloyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320L/M293P]

ATP + 3,4_Dihydroxy_trans_cinnamate + Coenzyme_A -> Diphosphate + Caffeoyl_CoA + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320L]

3,4_Dihydroxy_trans_cinnamate + Coenzyme_A + ATP -> AMP + Caffeoyl_CoA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293Y]

3,4_Dihydroxy_trans_cinnamate + ATP + Coenzyme_A -> AMP + Caffeoyl_CoA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320A/M293P]

Coenzyme_A + ATP + 3,4_Dihydroxy_trans_cinnamate -> Diphosphate + AMP + Caffeoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant M293A/V294L]

ATP + Coenzyme_A + Ferulate -> Feruloyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320L]

ATP + Coenzyme_A + Ferulate -> Feruloyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320L/M293P]

Coenzyme_A + ATP + Ferulate -> AMP + Diphosphate + Feruloyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320A]

Coenzyme_A + ATP + Ferulate -> Diphosphate + AMP + Feruloyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) mutant K320A/M293P]

Ferulate + ATP + Coenzyme_A -> AMP + Feruloyl_CoA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 36
uniprot:Q42524 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL1]

4_Coumarate + Coenzyme_A + ATP -> 4_Coumaroyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

Coenzyme_A + 4_Coumarate + ATP -> AMP + Diphosphate + 4_Coumaroyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q42524 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL1]

Coenzyme_A + Ferulate + ATP -> Feruloyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

ATP + Coenzyme_A + 3,4_Dihydroxy_trans_cinnamate -> Caffeoyl_CoA + AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q42524 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL1]

3,4_Dihydroxy_trans_cinnamate + ATP + Coenzyme_A -> AMP + Diphosphate + Caffeoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q42524 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL1]

ATP + trans_Cinnamate + Coenzyme_A -> Cinnamoyl_CoA + Diphosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S725 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL2]

ATP + Coenzyme_A + trans_Cinnamate -> AMP + Diphosphate + Cinnamoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S777 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL3]

ATP + 4_Coumarate + Coenzyme_A -> AMP + 4_Coumaroyl_CoA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S777 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL3]

ATP + Ferulate + Coenzyme_A -> Diphosphate + Feruloyl_CoA + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S777 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL3]

3,4_Dihydroxy_trans_cinnamate + ATP + Coenzyme_A -> Diphosphate + AMP + Caffeoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
uniprot:Q9S777 [4-coumarate---CoA ligase(Enzyme) wildtype At4CL3]

ATP + Coenzyme_A + trans_Cinnamate -> AMP + Cinnamoyl_CoA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 22
- [4-coumarate---CoA ligase(Enzyme) wildtype]

4_Coumarate + ATP + Coenzyme_A -> 4_Coumaroyl_CoA + AMP + Diphosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30
- [4-coumarate---CoA ligase(Enzyme) wildtype]

4_Coumarate + ATP + Coenzyme_A -> Diphosphate + 4_Coumaroyl_CoA + AMP		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 400 mM Tris-HCl, 5 mM MgCl2
PH: 7.8
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-1121 suberin monomers biosynthesis
BioCyc:META_PWY1F-FLAVSYN flavonoid biosynthesis
BioCyc:META_PWY-7397 naringenin biosynthesis (engineered)
BioCyc:ARA_PWY-361 phenylpropanoid biosynthesis
BioCyc:ARA_PWY-7398 coumarins biosynthesis (engineered)
BioCyc:META_PWY-6733 sporopollenin precursors biosynthesis
BioCyc:META_PWY-7398 coumarins biosynthesis (engineered)
BioCyc:ARA_PWY1F-FLAVSYN flavonoid biosynthesis
BioCyc:ARA_PWY-5868 simple coumarins biosynthesis
BioCyc:ARA_PWY-6803 phosphatidylcholine acyl editing
BioCyc:META_PWY-6443 benzoate biosynthesis I (CoA-dependent, β-oxidative)
BioCyc:META_PWY-6457 trans-cinnamoyl-CoA biosynthesis
BioCyc:ARA_PWY-735 jasmonic acid biosynthesis
BioCyc:ARA_PWY-5143 long-chain fatty acid activation
BioCyc:ARA_PWY-6733 sporopollenin precursors biosynthesis
BioCyc:META_PWY-361 phenylpropanoid biosynthesis
BioCyc:ARA_PWY-1121 suberin monomers biosynthesis
BioCyc:ARA_PWY-6982 umbelliferone biosynthesis
BioCyc:ARA_PWY-6673 caffeoylglucarate biosynthesis
Plant Reactome:R-OSA-9030769 Growth and developmental processes
Plant Reactome:R-OSA-9031670 Vegetative structure development
Plant Reactome:R-OSA-9627657 Regulation of leaf development
PlantCyc:ARA_PWY-6982 umbelliferone biosynthesis
PlantCyc:ARA_PWY-1121 suberin monomers biosynthesis
PlantCyc:PLANT_PWY1F-FLAVSYN flavonoid biosynthesis
PlantCyc:ARA_PWY-7398 coumarins biosynthesis (engineered)
PlantCyc:ARA_PWY-5868 simple coumarins biosynthesis
PlantCyc:PLANT_PWY-7397 naringenin biosynthesis (engineered)
PlantCyc:PLANT_PWY-5868 simplecoumarins biosynthesis
PlantCyc:PLANT_PWY-1121 suberin monomers biosynthesis
PlantCyc:PLANT_PWY-6733 sporopollenin precursors biosynthesis
PlantCyc:ARA_PWY1F-FLAVSYN flavonoid biosynthesis
PlantCyc:ARA_PWY-5143 long-chain fatty acid activation
PlantCyc:ARA_PWY-6673 caffeoylglucarate biosynthesis
PlantCyc:ARA_PWY-361 phenylpropanoid biosynthesis
PlantCyc:ARA_PWY-6803 phosphatidylcholine acyl editing
PlantCyc:PLANT_PWY-7398 coumarins biosynthesis (engineered)
PlantCyc:ARA_PWY-6733 sporopollenin precursors biosynthesis
PlantCyc:ARA_PWY-735 jasmonic acid biosynthesis
PlantCyc:PLANT_PWY-6443 benzoate biosynthesis I (CoA-dependent, β-oxidative)
PlantCyc:PLANT_PWY-5143 long-chain fatty acid activation
PlantCyc:PLANT_PWY-361 phenylpropanoid biosynthesis
PathBank:SMP0012024 Flavanone Biosynthesis