EC: 6.1.1.4

leucine-tRNA ligase (L-leucine:tRNALeu ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
- [Bovine serum albumin]

L_Leucine + tRNA(Leu) + ATP -> L_Leucyl_tRNA(Leu) + AMP + Diphosphate
(( (( (kcat * E) ) * S) ) / Km) buffer: 100 mM Hepes, 125 mM dipotassium glutarate, 250 mM KCl, 10 mM MgCl2, 10 mM dithiothreitol
PH: 7.5
Temperature: 50
uniprot:O27552 [Bovine serum albumin]

tRNA(Leu) + ATP + L_Leucine -> L_Leucyl_tRNA(Leu) + Diphosphate + AMP
(( (( (kcat * E) ) * S) ) / Km) buffer: 100 mM Hepes, 125 mM dipotassium glutarate, 250 mM KCl, 10 mM MgCl2, 10 mM dithiothreitol
PH: 7.5
Temperature: 50
uniprot:O27552 [Bovine serum albumin]

tRNA(Leu) + ATP + L_Leucine -> AMP + L_Leucyl_tRNA(Leu) + Diphosphate
(( (( (kcat * E) ) * S) ) / Km) buffer: 100 mM Hepes, 125 mM dipotassium glutarate, 250 mM KCl, 10 mM MgCl2, 10 mM dithiothreitol
PH: 7.5
Temperature: 50
- [Bovine serum albumin]

tRNA(Leu) + L_Leucine + ATP -> L_Leucyl_tRNA(Leu) + AMP + Diphosphate
(( (( (kcat * E) ) * S) ) / Km) buffer: 100 mM Hepes, 125 mM dipotassium glutarate, 250 mM KCl, 10 mM MgCl2, 10 mM dithiothreitol
PH: 7.5
Temperature: 50

Pathways

pathway id name
BioCyc:ANTHRA_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULONA1000_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:BTHE_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOO157_TRNA-CHARGING-PWY tRNA charging
BioCyc:SYNEL_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:PDIF272563_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:GCF_000013425_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
Reactome:R-DDI-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-DDI-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-DDI-1266738 Developmental Biology
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-72766 Translation
Reactome:R-MMU-1266738 Developmental Biology
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2408522 Selenoamino acid metabolism
Reactome:R-HSA-379724 tRNA Aminoacylation
PathBank:SMP0111873 Protein Synthesis: Leucine
PathBank:SMP0119316 Protein Synthesis: Leucine
PathBank:SMP0121283 tRNA Charging 2
PathBank:SMP0121281 tRNA Charging