EC: 6.1.1.17

glutamate-tRNA ligase (L-glutamate:tRNAGlu ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P04805 [glutamate---tRNA ligase(Enzyme) wildtype]

tRNA(Glu) + ATP + L_Glutamate -> AMP + Diphosphate + L_Glutamyl_tRNA(Glu)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Na-Hepes, 10 mM MgCl2, 30 mM KCl
PH: 7.2
Temperature: 36
uniprot:P04805 [glutamate---tRNA ligase(Enzyme) wildtype]

tRNA(Glu) + L_Glutamate + ATP -> AMP + Diphosphate + L_Glutamyl_tRNA(Glu)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Na-Hepes, 10 mM MgCl2, 30 mM KCl
PH: 7.2
Temperature: 36
uniprot:P22250 [Regulatory factor beta]

L_Glutamate + tRNA(Glu) + ATP -> AMP + L_Glutamyl_tRNA(Glu) + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Sodium HEPES, 10 mM KCl, 10 mM MgCl2
PH: 7.2
Temperature: 30
uniprot:P22250 [glutamate---tRNA ligase(Enzyme) wildtype]

tRNA(Glu) + ATP + L_Glutamate -> AMP + Diphosphate + L_Glutamyl_tRNA(Glu)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Sodium HEPES, 10 mM KCl, 10 mM MgCl2
PH: 7.2
Temperature: 30
uniprot:P04805 [glutamate---tRNA ligase(Enzyme) wildtype]

tRNA(Glu) + L_Glutamate + ATP -> L_Glutamyl_tRNA(Glu) + Diphosphate + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Sodium HEPES, 10 mM KCl, 10 mM MgCl2
PH: 7.2
Temperature: 30
uniprot:Q8DLI5 [glutamate---tRNA ligase(Enzyme) wildtype C-terminal His-tag]

L_Glutamate + ATP + tRNA(Glu) -> AMP + L_Glutamyl_tRNA(Glu) + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2, 5 mM DTT
PH: 7.5
Temperature: 37
uniprot:Q8DLI5 [glutamate---tRNA ligase(Enzyme) wildtype C-terminal His-tag]

ATP + tRNA(Gln) + L_Glutamate -> AMP + Diphosphate + L_Glutamyl_tRNA(Gln)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2, 5 mM DTT
PH: 7.5
Temperature: 37
uniprot:P04805 [glutamate---tRNA ligase(Enzyme) wildtype]

ATP + tRNA(Glu) + L_Glutamate -> Diphosphate + L_Glutamyl_tRNA(Glu) + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium Hepes, 16 mM MgCl, 0.8 mM DTT
PH: 7.2
Temperature: 37
uniprot:P04805 [glutamate---tRNA ligase(Enzyme) mutant C100Y]

L_Glutamate + tRNA(Glu) + ATP -> Diphosphate + AMP + L_Glutamyl_tRNA(Glu)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium Hepes, 16 mM MgCl, 0.8 mM DTT
PH: 7.2
Temperature: 37
uniprot:P04805 [glutamate---tRNA ligase(Enzyme) wildtype]

L_Glutamate + tRNA(Glu) + ATP -> L_Glutamyl_tRNA(Glu) + AMP + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium Hepes, 16 mM MgCl, 0.8 mM DTT
PH: 7.2
Temperature: 37
uniprot:P04805 [glutamate---tRNA ligase(Enzyme) mutant C100Y]

L_Glutamate + ATP + tRNA(Glu) -> AMP + Diphosphate + L_Glutamyl_tRNA(Glu)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium Hepes, 16 mM MgCl, 0.8 mM DTT
PH: 7.2
Temperature: 37
uniprot:P27000 [glutamate---tRNA ligase(Enzyme) wildtype]

L_Glutamate + tRNA(Glu) + ATP -> L_Glutamyl_tRNA(Glu) + Diphosphate + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes/NaOH, 20 mM MgCl2
PH: 7.2
Temperature: 65
uniprot:P27000 [glutamate---tRNA ligase(Enzyme) mutant R358Q]

tRNA(Glu) + L_Glutamate + ATP -> Diphosphate + AMP + L_Glutamyl_tRNA(Glu)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes/NaOH, 20 mM MgCl2
PH: 7.2
Temperature: 65
uniprot:P27000 [glutamate---tRNA ligase(Enzyme) wildtype]

ATP + L_Glutamate + tRNA(Glu) -> AMP + L_Glutamyl_tRNA(Glu) + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes/NaOH, 20 mM MgCl2
PH: 7.2
Temperature: 65
uniprot:P27000 [glutamate---tRNA ligase(Enzyme) mutant R358Q]

L_Glutamate + ATP + tRNA(Glu) -> Diphosphate + L_Glutamyl_tRNA(Glu) + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes/NaOH, 20 mM MgCl2
PH: 7.2
Temperature: 65

Pathways

pathway id name
BioCyc:MTBCDC1551_PWY-5918 superpathay of heme biosynthesis from glutamate
BioCyc:HPY_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:HPY_PWY-5921 L-glutamine biosynthesis II (tRNA-dependent)
BioCyc:CAULO_PWY-5918 superpathay of heme biosynthesis from glutamate
BioCyc:CAULO_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:ANTHRA_TRNA-CHARGING-PWY tRNA charging
BioCyc:BTHE_TRNA-CHARGING-PWY tRNA charging
BioCyc:CORYNE_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:CORYNE_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:GCF_000013425_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:META_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOO157_TRNA-CHARGING-PWY tRNA charging
BioCyc:ANTHRA_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:SYNEL_TRNA-CHARGING-PWY tRNA charging
BioCyc:SYNEL_P381-PWY adenosylcobalamin biosynthesis II (late cobalt incorporation)
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:PDIF272563_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECO_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL199310_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:BSUB_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:SYNEL_PWY-5918 superpathay of heme biosynthesis from glutamate
BioCyc:SYNEL_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:GCF_000013425_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_PWY-5918 superpathay of heme b biosynthesis from glutamate
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:MTBCDC1551_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:ECOO157_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:ECOL199310_PWY-5188 tetrapyrrole biosynthesis I (from glutamate)
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:SYNEL_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
BioCyc:GCF_000013425_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
PathBank:SMP0000953 Porphyrin Metabolism
Reactome:R-MMU-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-DME-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-HSA-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9730414 MITF-M-regulated melanocyte development
Reactome:R-DME-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-DME-1266738 Developmental Biology
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-HSA-72306 tRNA processing
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-72766 Translation
Reactome:R-HSA-8953854 Metabolism of RNA
Reactome:R-MMU-1266738 Developmental Biology
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2408522 Selenoamino acid metabolism
Reactome:R-HSA-379724 tRNA Aminoacylation
Reactome:R-HSA-6782315 tRNA modification in the nucleus and cytosol
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0111864 Protein Synthesis: Glutamic Acid
PathBank:SMP0112609 Protein Synthesis: Proline
PathBank:SMP0119312 Protein Synthesis: Glutamic Acid
PathBank:SMP0119320 Protein Synthesis: Proline
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]