EC: 6.1.1.14

glycine-tRNA ligase (glycine:tRNAGly ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P41250 [glycine---tRNA ligase(Enzyme) wildtype C-terminal His-tagged]

Glycine + ATP + tRNA(Gly) -> AMP + Diphosphate + Glycyl_tRNA(Gly)		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 20 mM KCl, 10 mM MgCl2, 4 mM DTT
PH: 7.5
Temperature: 37
uniprot:P41250 [glycine---tRNA ligase(Enzyme) mutant R310Q C-terminal His-tagged]

ATP + Glycine + tRNA(Gly) -> Glycyl_tRNA(Gly) + Diphosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 20 mM KCl, 10 mM MgCl2, 4 mM DTT
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:VCHO_TRNA-CHARGING-PWY tRNA charging
BioCyc:ANTHRA_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULONA1000_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOO157_TRNA-CHARGING-PWY tRNA charging
BioCyc:SYNEL_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:PDIF272563_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL199310_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:GCF_000013425_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-72766 Translation
Reactome:R-HSA-379724 tRNA Aminoacylation
PathBank:SMP0120694 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120754 Non-Ketotic Hyperglycinemia
PathBank:SMP0120770 Hyperglycinemia, Non-Ketotic
PathBank:SMP0111870 Protein Synthesis: Glycine
PathBank:SMP0119313 Protein Synthesis: Glycine
PathBank:SMP0119469 Protein Synthesis: Glycine
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0087338 Glycine and Serine Metabolism
PathBank:SMP0087466 Glycine and Serine Metabolism
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120741 Sarcosinemia
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0121283 tRNA Charging 2
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120872 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0121281 tRNA Charging
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0120695 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0087399 Glycine and Serine Metabolism
PathBank:SMP0063626 Glycine and Serine Metabolism