EC: 5.3.3.5

cholestenol DELTA-isomerase (DELTA7-Cholestenol DELTA7-DELTA8-isomerase)

enzyme lambda metabolic reaction experiment
uniprot:Q9JJ46 [cholestenol Delta-isomerase(Enzyme) wildtype]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM reduced Glutathione, 3 mM Nicotinamide, 25 mg/ml Glucose, 20 units/ml Glucose oxidase, 0.1 M Potassium phosphate, 20% Glycerol, 0.5 mM EDTA
PH: 7.4
Temperature: 37
uniprot:Q15125 [Ketoconazole]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (V / (1 + (( (Km / S) ) * (1 + (I / Ki))))) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [Tamoxifen]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (V / (1 + (( (Km / S) ) * (1 + (I / Ki))))) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [cholestenol Delta-isomerase(Enzyme) wildtype]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (( (V * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [cholestenol Delta-isomerase(Enzyme) wildtype]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (( (V * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [Cholesterol]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (V / (1 + (( (Km / S) ) * (1 + (I / Ki))))) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [25-Azacholesterol]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (V / (1 + (( (Km / S) ) * (1 + (I / Ki))))) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [Emopamil]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (V / (1 + (( (Km / S) ) * (1 + (I / Ki))))) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [Tridemorph]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (V / (1 + (( (Km / S) ) * (1 + (I / Ki))))) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [cholestenol Delta-isomerase(Enzyme) wildtype]

Zymosterol -> 5alpha_Cholesta_7,24_dien_3beta_ol		 (( (V * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37
uniprot:Q15125 [cholestenol Delta-isomerase(Enzyme) wildtype]

5alpha_Cholesta_7,24_dien_3beta_ol -> Zymosterol		 (( (V * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 2 mM MgCl2, 1 mM EDTA, 2 mM 2-mercaptoethanol, 5 % glycerol
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY66-3 cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY66-341 cholesterol biosynthesis I
BioCyc:META_PWY66-341 cholesterol biosynthesis I
BioCyc:META_PWY66-4 cholesterol biosynthesis III (via desmosterol)
BioCyc:ARA_PWY-2541 plant sterol biosynthesis
BioCyc:MOUSE_PWY66-341 cholesterol biosynthesis I
BioCyc:HUMAN_PWY66-3 cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
BioCyc:META_PWY66-3 cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
BioCyc:HUMAN_PWY66-4 cholesterol biosynthesis III (via desmosterol)
BioCyc:META_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:META_PWY-2541 plant sterol biosynthesis
BioCyc:MOUSE_PWY66-4 cholesterol biosynthesis III (via desmosterol)
Plant Reactome:R-OSA-1119559 Cholesterol biosynthesis I
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744344 Secondary metabolism
Plant Reactome:R-OSA-1119370 Sterol biosynthesis
Plant Reactome:R-OSA-1119286 Cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
Plant Reactome:R-OSA-3906998 Fatty acid and lipid metabolism
Plant Reactome:R-OSA-1119439 Cholesterol biosynthesis III (via desmosterol)
PlantCyc:ARA_PWY-2541 phytosterol biosynthesis (plants)
PlantCyc:PLANT_PWY-2541 phytosterol biosynthesis (plants)
Reactome:R-RNO-9969901 Cholesterol biosynthesis from zymosterol (modified Kandutsch-Russell pathway)
Reactome:R-HSA-9969901 Cholesterol biosynthesis from zymosterol (modified Kandutsch-Russell pathway)
Reactome:R-MMU-9969901 Cholesterol biosynthesis from zymosterol (modified Kandutsch-Russell pathway)
Reactome:R-DDI-9969901 Cholesterol biosynthesis from zymosterol (modified Kandutsch-Russell pathway)
Reactome:R-DDI-191273 Cholesterol biosynthesis
Reactome:R-HSA-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-191273 Cholesterol biosynthesis
Reactome:R-RNO-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-DDI-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-MMU-191273 Cholesterol biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-MMU-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-RNO-191273 Cholesterol biosynthesis
WikiPathways:WP5064 7-oxo-C and 7-beta-HC pathways
WikiPathways:WP4545 Oxysterols derived from cholesterol
PathBank:SMP0121057 Bloch Pathway (Cholesterol Biosynthesis)
PathBank:SMP0000023 Steroid Biosynthesis
PathBank:SMP0000387 CHILD Syndrome
PathBank:SMP0000112 Risedronate Action Pathway
PathBank:SMP0000508 Cholesteryl Ester Storage Disease
PathBank:SMP0000389 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0000319 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0000082 Simvastatin Action Pathway
PathBank:SMP0000111 Cerivastatin Action Pathway
PathBank:SMP0000131 Atorvastatin Action Pathway
PathBank:SMP0000511 Wolman Disease
PathBank:SMP0121060 Kandutsch-Russell Pathway (Cholesterol Biosynthesis)
PathBank:SMP0000386 Desmosterolosis
PathBank:SMP0000089 Pravastatin Action Pathway
PathBank:SMP0000095 Alendronate Action Pathway
PathBank:SMP0000099 Lovastatin Action Pathway
PathBank:SMP0000117 Pamidronate Action Pathway
PathBank:SMP0000509 Hyper-IgD Syndrome
PathBank:SMP0121236 Steroid Biosynthesis
PathBank:SMP0000388 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0000079 Ibandronate Action Pathway
PathBank:SMP0000092 Rosuvastatin Action Pathway
PathBank:SMP0000209 Hypercholesterolemia
PathBank:SMP0000107 Zoledronate Action Pathway
PathBank:SMP0000119 Fluvastatin Action Pathway
PathBank:SMP0000510 Mevalonic Aciduria