EC: 5.3.1.4

L-arabinose isomerase (beta-L-arabinopyranose aldose-ketose-isomerase)

enzyme lambda metabolic reaction experiment
uniprot:Q2VMT2 [L-arabinose isomerase(Enzyme) mutant K269E]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium acetate, 1 mM MnCl2, 1 mM CoCl2
PH: 6
Temperature: 65
uniprot:Q2VMT2 [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium acetate, 1 mM MnCl2, 1 mM CoCl2
PH: 6
Temperature: 65
uniprot:Q2VMT2 [L-arabinose isomerase(Enzyme) wildtype]

D_Galactose -> D_Tagatose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium acetate, 1 mM MnCl2, 1 mM CoCl2
PH: 6
Temperature: 65
uniprot:Q2VMT2 [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM MnCl2, 1 mM CoCl2
PH: 8
Temperature: 65
uniprot:Q9KBQ2 [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium acetate, 1 mM MnCl2, 1 mM CoCl2
PH: 6
Temperature: 50
uniprot:Q2VMT2 [L-arabinose isomerase(Enzyme) mutant K269E]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 1 mM MnCl2, 1 mM CoCl2
PH: 7
Temperature: 65
uniprot:Q2VMT2 [L-arabinose isomerase(Enzyme) mutant K269E]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM MnCl2, 1 mM CoCl2
PH: 8
Temperature: 65
uniprot:Q2VMT2 [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 1 mM MnCl2, 1 mM CoCl2
PH: 7
Temperature: 65
uniprot:Q9KBQ2 [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM MnCl2, 1 mM CoCl2
PH: 8
Temperature: 50
uniprot:Q9KBQ2 [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 1 mM MnCl2, 1 mM CoCl2
PH: 7
Temperature: 50
uniprot:Q9KBQ2 [L-arabinose isomerase(Enzyme) mutant E268K]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 1 mM MnCl2, 1 mM CoCl2
PH: 7
Temperature: 50
uniprot:Q9KBQ2 [L-arabinose isomerase(Enzyme) mutant E268K]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium acetate, 1 mM MnCl2, 1 mM CoCl2
PH: 6
Temperature: 50
uniprot:Q9KBQ2 [L-arabinose isomerase(Enzyme) mutant E268K]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM MnCl2, 1 mM CoCl2
PH: 8
Temperature: 50
- [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * S) ) / (Km + S)) buffer: 45 mM Tris
PH: 7.5
Temperature: 23
- [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris
PH: 7.5
Temperature: 23
uniprot:A9J246 [L-arabinose isomerase(Enzyme) wildtype]

L_Arabinose -> L_Ribulose		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MOPS, 0.5 mM Co2+, 1 mM Mn2+
PH: 7.5
Temperature: 60
uniprot:A9J246 [L-arabinose isomerase(Enzyme) wildtype]

D_Galactose -> D_Tagatose		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MOPS, 0.5 mM Co2+, 1 mM Mn2+
PH: 7.5
Temperature: 60
uniprot:Q9S467 [Co2+]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM MOPS
PH: 7.5
Temperature: 80
uniprot:Q9S467 [Co2+]

D_Galactose -> D_Tagatose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM MOPS
PH: 7.5
Temperature: 80
uniprot:Q9WYB3 [Co2+]

L_Arabinose -> L_Ribulose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.5
Temperature: 90
uniprot:Q9WYB3 [Co2+]

D_Galactose -> D_Tagatose		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.5
Temperature: 90

Pathways

pathway id name
BioCyc:BSUB_ARABCAT-PWY L-arabinose degradation I
BioCyc:META_PWY-5257 superpathway of pentose and pentitol degradation
BioCyc:ECO_ARABCAT-PWY L-arabinose degradation I
BioCyc:ECOO157_ARABCAT-PWY L-arabinose degradation I
BioCyc:META_ARABCAT-PWY L-arabinose degradation I
BioCyc:ECOL316407_ARABCAT-PWY L-arabinose degradation I
BioCyc:ECOL199310_ARABCAT-PWY L-arabinose degradation I
BioCyc:BTHE_ARABCAT-PWY L-arabinose degradation I
PathBank:SMP0001892 Operon: Arabinose Activation
PathBank:SMP0002115 L-Arabinose Degradation I