EC: 4.6.1.2

guanylate cyclase (GTP diphosphate-lyase (cyclizing; 3',5'-cyclic-GMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q553Y7 [GTP-gamma-S]

GTP -> 3',5'_Cyclic_GMP + Diphosphate		 (( (Vmax * (A ^ n)) ) / (( (Ka ^ n) ) + (A ^ n))) buffer: 15 mM Tris, 10 mM dithiothreitol, 0.2 mM 3-isobutyl-1-methylxanthine, 0.75 mM EGTA, 250 mM sucrose
PH: 8
Temperature: 22
uniprot:Q963L5 [GTP-gamma-S]

GTP -> 3',5'_Cyclic_GMP + Diphosphate		 (( (Vmax * (A ^ n)) ) / (( (Ka ^ n) ) + (A ^ n))) buffer: 15 mM Tris, 10 mM dithiothreitol, 0.2 mM 3-isobutyl-1-methylxanthine, 0.75 mM EGTA, 250 mM sucrose
PH: 8
Temperature: 22
uniprot:Q553Y7 [GTP-gamma-S]

GTP -> Diphosphate + 3',5'_Cyclic_GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM Tris, 10 mM dithiothreitol, 0.2 mM 3-isobutyl-1-methylxanthine, 0.75 mM EGTA, 250 mM sucrose
PH: 8
Temperature: 22
uniprot:Q963L5 [GTP-gamma-S]

GTP -> Diphosphate + 3',5'_Cyclic_GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM Tris, 10 mM dithiothreitol, 0.2 mM 3-isobutyl-1-methylxanthine, 0.75 mM EGTA, 250 mM sucrose
PH: 8
Temperature: 22
uniprot:Q963L5 [Mg2+]

GTP -> 3',5'_Cyclic_GMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM Tris, 10 mM dithiothreitol, 0.2 mM 3-isobutyl-1-methylxanthine, 0.75 mM EGTA, 250 mM sucrose
PH: 8
Temperature: 22
uniprot:Q553Y7 [Mg2+]

GTP -> Diphosphate + 3',5'_Cyclic_GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM Tris, 10 mM dithiothreitol, 0.2 mM 3-isobutyl-1-methylxanthine, 0.75 mM EGTA, 250 mM sucrose
PH: 8
Temperature: 22
uniprot:Q553Y7 [GTP-gamma-S]

GTP -> 3',5'_Cyclic_GMP + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes/NaOH, 1 mM Dithiothreitol, 3 mM MgSO4, 3 mM EGTA
PH: 7
Temperature: 36
uniprot:Q553Y7 [guanylate cyclase(Enzyme) wildtype DdGCA]

GTP -> 3',5'_Cyclic_GMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mm Hepes, 3 mm EGTA, 3 mm Mg2+, 10 mm dithiothreitol
PH: 7
Temperature: 36
uniprot:Q553Y7 [GTP-gamma-S]

GTP -> 3',5'_Cyclic_GMP + Diphosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mm Hepes, 3 mm EGTA, 3 mm Mg2+, 10 mm dithiothreitol
PH: 7
Temperature: 36
uniprot:Q553Y7 [guanylate cyclase(Enzyme) wildtype DdGCA]

GTP_gamma_S -> 3',5'_Cyclic_GMP + Thiopyrophosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mm Hepes, 3 mm EGTA, 3 mm Mg2+, 10 mm dithiothreitol
PH: 7
Temperature: 36
uniprot:Q553Y7 [GTP-gamma-S]

GTP -> Diphosphate + 3',5'_Cyclic_GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Hepes/NaOH, 1.5 mM MgSO4, 0.5 mM EGTA, 5E-5 M App(NH)p, 5 mM DTT
PH: 7
Temperature: 25
uniprot:Q553Y7 [guanylate cyclase(Enzyme) wildtype]

GTP -> Diphosphate + 3',5'_Cyclic_GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Hepes/NaOH, 1.5 mM MgSO4, 0.5 mM EGTA, 5E-5 M App(NH)p, 5 mM DTT
PH: 7
Temperature: 25
uniprot:Q553Y7 [guanylate cyclase(Enzyme) wildtype]

GTP_gamma_S -> Thiopyrophosphate + 3',5'_Cyclic_GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Hepes/NaOH, 1.5 mM MgSO4, 0.5 mM EGTA, 5E-5 M App(NH)p, 5 mM DTT
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:MOUSE_PWY3DJ-7 cyclic GMP biosynthesis
Reactome:R-HSA-9824439 Bacterial Infection Pathways
Reactome:R-BTA-5576891 Cardiac conduction
Reactome:R-HSA-5578768 Physiological factors
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-5339562 Uptake and actions of bacterial toxins
Reactome:R-HSA-8942233 Intestinal infectious diseases
Reactome:R-MMU-5578768 Physiological factors
Reactome:R-BTA-5578768 Physiological factors
Reactome:R-DME-445355 Smooth Muscle Contraction
Reactome:R-HSA-8935690 Digestion
Reactome:R-RNO-397014 Muscle contraction
Reactome:R-BTA-397014 Muscle contraction
Reactome:R-HSA-5576891 Cardiac conduction
Reactome:R-MMU-5576891 Cardiac conduction
Reactome:R-MMU-8963743 Digestion and absorption
Reactome:R-RNO-8963743 Digestion and absorption
Reactome:R-RNO-5578768 Physiological factors
Reactome:R-SSC-8963743 Digestion and absorption
Reactome:R-DME-397014 Muscle contraction
Reactome:R-HSA-8963743 Digestion and absorption
Reactome:R-HSA-397014 Muscle contraction
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-397014 Muscle contraction
Reactome:R-MMU-8935690 Digestion
Reactome:R-RNO-8935690 Digestion
Reactome:R-RNO-5576891 Cardiac conduction
Reactome:R-SSC-8935690 Digestion
WikiPathways:WP4746 Thyroid hormones production and peripheral downstream signaling effects
PathBank:SMP0108237 Ion Channels and Their Functional Role in Vascular Endothelium
PathBank:SMP0119374 Circadian Rhythms
PathBank:SMP0119382 Circadian Rhythms
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0084271 NAD+ Signalling and Aging
PathBank:SMP0108160 Ion Channels and Their Functional Role in Vascular Endothelium
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0090831 Circadian Rhythms
PathBank:SMP0063778 Ion Channels and Their Functional Role in Vascular Endothelium
PathBank:SMP0108207 Ion Channels and Their Functional Role in Vascular Endothelium
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0087239 Purine Metabolism