EC: 4.4.1.9

L-3-cyanoalanine synthase (L-cysteine hydrogen-sulfide-lyase (adding hydrogen cyanide; L-3-cyanoalanine-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q9S757 [Pyridoxal phosphate]

L_Cysteine + Cyanide -> Sulfide + 3_Cyano_L_alanine		 (( (( (kcat * E) ) * (A ^ h)) ) / (K + (A ^ h))) buffer: 25 mM CAPS
PH: 10
Temperature: 30
uniprot:Q9S757 [Pyridoxal phosphate]

Cyanide + L_Cysteine -> 3_Cyano_L_alanine + Sulfide		 (( (( (kcat * E) ) * (B ^ h)) ) / (K + (B ^ h))) buffer: 25 mM CAPS
PH: 10
Temperature: 30
uniprot:Q43153 [L-3-cyanoalanine synthase(Enzyme) wildtype]

L_Cysteine + Cyanide -> 3_Cyano_L_alanine + Hydrogen_sulfide		 (( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A)) buffer: 160 mM 2-amino 2-methyl 1-propanol
PH: 9.8
Temperature: 26
uniprot:P32260 [L-3-cyanoalanine synthase(Enzyme) wildtype CysSynB]

L_Cysteine + Cyanide -> Hydrogen_sulfide + 3_Cyano_L_alanine		 (( (Vmax * (A ^ n)) ) / (k + (A ^ n))) buffer: 160 mM 2-amino 2-methyl 1-propanol
PH: 9.8
Temperature: 26
uniprot:Q00834 [L-3-cyanoalanine synthase(Enzyme) wildtype CysSynA]

Cyanide + L_Cysteine -> Hydrogen_sulfide + 3_Cyano_L_alanine		 (( (Vmax * (A ^ n)) ) / (k + (A ^ n))) buffer: 160 mM 2-amino 2-methyl 1-propanol
PH: 9.8
Temperature: 26
uniprot:Q43153 [L-3-cyanoalanine synthase(Enzyme) wildtype]

Cyanide + L_Cysteine -> 3_Cyano_L_alanine + Hydrogen_sulfide		 (( (Vmax * A) ) / (Km + A)) buffer: 160 mM 2-amino 2-methyl 1-propanol
PH: 9.8
Temperature: 26
uniprot:Q00834 [L-3-cyanoalanine synthase(Enzyme) wildtype CysSynA]

L_Cysteine + Cyanide -> Hydrogen_sulfide + 3_Cyano_L_alanine		 (( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A)) buffer: 160 mM 2-amino 2-methyl 1-propanol
PH: 9.8
Temperature: 26
uniprot:P32260 [L-3-cyanoalanine synthase(Enzyme) wildtype CysSynB]

Cyanide + L_Cysteine -> Hydrogen_sulfide + 3_Cyano_L_alanine		 (( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A)) buffer: 160 mM 2-amino 2-methyl 1-propanol
PH: 9.8
Temperature: 26
uniprot:A0A1B4XK00 [L-3-cyanoalanine synthase(Enzyme) wildtype]

O_Acetyl_L_serine + Cyanide -> 3_Cyano_L_alanine + Acetate		 (( (Vmax * (B ^ n)) ) / (( (B_half ^ n) ) + (B ^ n))) buffer: 50 mM potassium phosphate, 80 µM PLP
PH: 7.5
Temperature: 30
uniprot:A0A1B4XK00 [L-3-cyanoalanine synthase(Enzyme) wildtype]

O_Acetyl_L_serine + Cyanide -> 3_Cyano_L_alanine + Acetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 80 µM PLP
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:ARA_ASPSYNII-PWY cyanide detoxification I
BioCyc:ARA_CYSTSYN-PWY L-cysteine biosynthesis I
BioCyc:META_ASPSYNII-PWY cyanide detoxification I
BioCyc:ARA_P401-PWY cyanide degradation
PlantCyc:ARA_P401-PWY cyanide degradation
PlantCyc:PLANT_P401-PWY cyanide degradation
PlantCyc:ARA_ASPSYNII-PWY cyanide detoxification I
PlantCyc:ARA_CYSTSYN-PWY L-cysteine biosynthesis I