EC: 4.2.1.33

3-isopropylmalate dehydratase ((2R,3S)-3-isopropylmalate hydro-lyase (2-isopropylmaleate-forming))

enzyme lambda metabolic reaction experiment
- [3-isopropylmalate dehydratase(Enzyme) wildtype MJ0499/MJ1277]

2_Isopropylmalate -> H2O + 2_Isopropylmaleate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM TES-KOH, 300 mM KCl, 10 mM MgCl2
PH: 7.5
Temperature: 60
- [3-isopropylmalate dehydratase(Enzyme) wildtype MJ0499/MJ1277]

3_Isopropylmalate -> 2_Isopropylmaleate + H2O
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM TES-KOH, 300 mM KCl, 10 mM MgCl2
PH: 7.5
Temperature: 60

Pathways

pathway id name
BioCyc:MTBH37RV_LEUSYN-PWY L-leucine biosynthesis
BioCyc:CAULO_LEUSYN-PWY leucine biosynthesis
BioCyc:ANTHRA_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:ANTHRA_LEUSYN-PWY L-leucine biosynthesis
BioCyc:BSUB_LEUSYN-PWY L-leucine biosynthesis
BioCyc:BTHE_LEUSYN-PWY leucine biosynthesis
BioCyc:BTHE_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:CORYNE_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:GCF_000013425_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:META_LEUSYN-PWY L-leucine biosynthesis
BioCyc:YEAST_LEUSYN-PWY leucine biosynthesis
BioCyc:ARA_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:VCHO_LEUSYN-PWY L-leucine biosynthesis
BioCyc:MTBCDC1551_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:ECOO157_LEUSYN-PWY leucine biosynthesis
BioCyc:ECOL199310_LEUSYN-PWY leucine biosynthesis
BioCyc:SYNEL_LEUSYN-PWY L-leucine biosynthesis
BioCyc:SYNEL_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:PDIF272563_LEUSYN-PWY L-leucine biosynthesis
BioCyc:META_PWY-5101 L-isoleucine biosynthesis II
BioCyc:ARA_LEUSYN-PWY L-leucine biosynthesis
BioCyc:VCHO_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:MTBH37RV_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:MTBCDC1551_LEUSYN-PWY L-leucine biosynthesis
BioCyc:ECOL199310_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:CAULO_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:AGRO_LEUSYN-PWY L-leucine biosynthesis
BioCyc:BSUB_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:ECOL316407_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:CAULONA1000_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:PDIF272563_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:CORYNE_LEUSYN-PWY L-leucine biosynthesis
BioCyc:YEAST_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:ECOO157_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:AGRO_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:ECOL316407_LEUSYN-PWY leucine biosynthesis
BioCyc:CAULONA1000_LEUSYN-PWY leucine biosynthesis
BioCyc:GCF_000013425_LEUSYN-PWY L-leucine biosynthesis
PathBank:SMP0000831 Leucine Biosynthesis
PathBank:SMP0000994 Secondary Metabolites: Valine and L-Leucine Biosynthesis from Pyruvate
PathBank:SMP0000996 Secondary Metabolites: Leucine Biosynthesis
PlantCyc:ARA_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
PlantCyc:ARA_LEUSYN-PWY L-leucine biosynthesis
PlantCyc:PLANT_LEUSYN-PWY L-leucine biosynthesis
PathBank:SMP0121332 Secondary Metabolites: Leucine Biosynthesis
PathBank:SMP0121340 TCA Cycle (Ubiquinol-5)
PathBank:SMP0121344 TCA Cycle (Ubiquinol-9)
PathBank:SMP0121287 Leucine Biosynthesis
PathBank:SMP0121331 Secondary Metabolites: Valine and L-Leucine Biosynthesis from Pyruvate
PathBank:SMP0121339 TCA Cycle (Ubiquinol-4)
PathBank:SMP0121343 TCA Cycle (Ubiquinol-8)
PathBank:SMP0122219 TCA Cycle (Ubiquinol-0)
PathBank:SMP0121204 L-Homomethionine Biosynthesis
PathBank:SMP0121269 TCA Cycle
PathBank:SMP0121337 TCA Cycle (Ubiquinol-2)
PathBank:SMP0121341 TCA Cycle (Ubiquinol-6)
PathBank:SMP0121345 TCA Cycle (Ubiquinol-10)
PathBank:SMP0012440 C5-Branched Dibasic Acid Metabolism
PathBank:SMP0121322 Propanoate Metabolism
PathBank:SMP0121326 Secondary Metabolites: Glyoxylate Cycle
PathBank:SMP0121338 TCA Cycle (Ubiquinol-3)
PathBank:SMP0121342 TCA Cycle (Ubiquinol-7)
PathBank:SMP0122218 Glycolate and Glyoxylate Degradation II