EC: 4.2.1.1

carbonic anhydrase (carbonic acid hydro-lyase (carbon-dioxide-forming))

enzyme lambda metabolic reaction experiment
uniprot:P00918 [carbonate dehydratase(Enzyme) mutant T199D]

H2O + CO2 -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS or bicine, 40 µM m-cresol purple
PH: 9
Temperature: 36
uniprot:P00918 [carbonate dehydratase(Enzyme) mutant T199E]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS or bicine, 40 µM m-cresol purple
PH: 9
Temperature: 36
- [H+]

CO2 + H2O -> Dihydrogen_carbonate		 (E * (( (kcat_1 / (1 + (H / K_1))) ) + (kcat_2 / (1 + (H / K_2))))) buffer: 40 mM buffer (Mes, pH 6.0-7.0; Mops, pH 7.0-8.0; bicine, pH 8.0-9.5), 30-80 µM indicator dye (chlorophenol red, p-nitrophenol, or m-cresol purple, respectively), 2.5 mM EDTA
PH: 7
Temperature: 25
- [H+]

CO2 + H2O -> Dihydrogen_carbonate		 (E * (( (kcat_1 / (1 + (H / K_1))) ) + (kcat_2 / (1 + (H / K_2))))) buffer: 40 mM buffer (Mes, pH 6.0-7.0; Mops, pH 7.0-8.0; bicine, pH 8.0-9.5), 30-80 µM indicator dye (chlorophenol red, p-nitrophenol, or m-cresol purple, respectively), 2.5 mM EDTA
PH: 7
Temperature: 25
- [Imidazole]

H2O + CO2 -> Dihydrogen_carbonate		 (E * (k_min + (( (( (k_max - k_min) ) * B) ) / (Kd + B)))) buffer: 40 mM Mops, 30 µM p-nitrophenol, 2.5 mM EDTA
PH: 7
Temperature: 25
- [Imidazole]

H2O + CO2 -> Dihydrogen_carbonate		 (E * (k_min + (( (( (k_max - k_min) ) * B) ) / (Kd + B)))) buffer: 40 mM Mops, 30 µM p-nitrophenol, 2.5 mM EDTA
PH: 7
Temperature: 25
- [H+]

H2O + CO2 -> Dihydrogen_carbonate		 (E * (( (kcat_1 / (1 + (H / K_1))) ) + (kcat_2 / (1 + (H / K_2))))) buffer: 40 mM buffer (Mes, pH 6.0-7.0; Mops, pH 7.0-8.0; bicine, pH 8.0-9.5), 30-80 µM indicator dye (chlorophenol red, p-nitrophenol, or m-cresol purple, respectively), 2.5 mM EDTA
PH: 7
Temperature: 25
- [carbonate dehydratase(Enzyme) mutant Y212F]

H2O + CO2 -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * A) ) / (( (Km + A) ) + (( (( (A ^ 2) ) / alpha) ) * (Km ^ 2)))) buffer: 40 mM Bicine, 30-80 µM m-cresol purple, 2.5 mM EDTA
PH: 9.25
Temperature: 25
- [carbonate dehydratase(Enzyme) mutant Y212F]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 40 mM Mops, 30-80 µM p-nitrophenol, 2.5 mM EDTA
PH: 7
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM 1,2-dimethylimidazole, 500 µM EDTA, 10 µM m-cresol purple
PH: 8.7
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM 1,2-dimethylimidazole, 500 µM EDTA, 25 µM m-cresol purple
PH: 8.39
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM 1,2-dimethylimidazole, 500 µM EDTA, 50 µM m-cresol purple
PH: 8.17
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM 1,2-dimethylimidazole, 500 µM EDTA, 10 µM m-cresol purple
PH: 8.88
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM 1,2-dimethylimidazole, 500 µM EDTA, 10 µM m-cresol purple
PH: 9.03
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM phosphate, 500 µM EDTA
PH: 6.26
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM phosphate, 500 µM EDTA
PH: 6.62
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM phosphate, 500 µM EDTA
PH: 6.26
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 12.5 mM phosphate, 500 µM EDTA
PH: 6.26
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM phosphate, 500 µM EDTA, 25 µM p-nitrophenol
PH: 7.43
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM 1,2-dimethylimidazole, 500 µM EDTA, 50 µM m-cresol purple
PH: 7.76
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM phosphate, 500 µM EDTA, 100 µM p-nitrophenol
PH: 6.25
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM phosphate, 500 µM EDTA, 50 µM p-nitrophenol
PH: 7
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM 1,2-dimethylimidazole, 500 µM EDTA, 50 µM m-cresol purple
PH: 7.96
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM phosphate, 500 µM EDTA, 50 µM p-nitrophenol
PH: 6.46
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM phosphate, 500 µM EDTA, 50 µM p-nitrophenol
PH: 6.87
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM phosphate, 500 µM EDTA, 50 µM p-nitrophenol
PH: 7.25
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 15 mM phosphate, 500 µM EDTA, 50 µM p-nitrophenol
PH: 6.67
Temperature: 25
uniprot:P00918 [carbonate dehydratase(Enzyme) mutant T199H]

CO2 + H2O -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS or bicine, 40 µM m-cresol purple
PH: 8
Temperature: 36
uniprot:P00918 [carbonate dehydratase(Enzyme) mutant T199H]

H2O + CO2 -> Dihydrogen_carbonate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS or bicine, 40 µM m-cresol purple
PH: 9
Temperature: 36
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 15 mM imidazole, 500 µM EDTA
PH: 7.11
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 15 mM imidazole, 500 µM EDTA
PH: 6.74
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 15 mM phosphate, 500 µM EDTA
PH: 6.62
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM imidazole, 500 µM EDTA
PH: 7.11
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

H2O + CO2 -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM imidazole, 500 µM EDTA
PH: 7.11
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 12.5 mM phosphate, 500 µM EDTA
PH: 6.62
Temperature: 25
uniprot:P16016 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM imidazole, 500 µM EDTA
PH: 6.74
Temperature: 25
- [carbonate dehydratase(Enzyme) mutant Q158A]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 40 mM Bicine, 30-80 µM indicator dye, 2.5 mM EDTA
PH: 8
Temperature: 25
uniprot:Q99N23 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM HEPES, 0.1 M Na2SO4, 0.2 mM phenol red
PH: 7.5
Temperature: 20
uniprot:Q8N1Q1 [carbonate dehydratase(Enzyme) wildtype]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM HEPES, 0.1 M Na2SO4, 0.2 mM phenol red
PH: 7.5
Temperature: 20
uniprot:Q3I4V7 [Histamine]

CO2 + H2O -> Dihydrogen_carbonate		 (( (E * kcat) ) / (1 + (( (Km / S) ) * (1 + (A / Ka))))) buffer: 20 mM Tris, 20 mM NaClO4, 0.2 mM Phenol red
PH: 8.3
Temperature: 25
uniprot:Q5AJ71 [Histamine]

H2O + CO2 -> Dihydrogen_carbonate		 (( (E * kcat) ) / (1 + (( (Km / S) ) * (1 + (A / Ka))))) buffer: 20 mM Tris, 20 mM NaClO4, 0.2 mM Phenol red
PH: 8.3
Temperature: 25
uniprot:Q3I4V7 [carbonate dehydratase(Enzyme) wildtype Can2]

H2O + CO2 -> Dihydrogen_carbonate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 20 mM NaClO4, 0.2 mM Phenol red
PH: 8.3
Temperature: 25
uniprot:Q5AJ71 [carbonate dehydratase(Enzyme) wildtype CaNce 103]

CO2 + H2O -> Dihydrogen_carbonate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 20 mM NaClO4, 0.2 mM Phenol red
PH: 8.3
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWYQT-4429 CO2 fixation into oxaloacetate (anaplerotic)
BioCyc:META_CYANCAT-PWY cyanate degradation
BioCyc:ECO_CYANCAT-PWY cyanate degradation
BioCyc:ARA_CYANCAT-PWY cyanate degradation
BioCyc:META_PWY-7926 carbon disulfide oxidation III (metazoa)
BioCyc:ECOO157_CYANCAT-PWY cyanate degradation
BioCyc:SYNEL_CYANCAT-PWY cyanate degradation
BioCyc:SYNEL_PWYQT-4429 CO2 fixation into oxaloacetate (anaplerotic)
BioCyc:ARA_PWYQT-4429 CO2 fixation into oxaloacetate (anaplerotic)
BioCyc:ECOL316407_CYANCAT-PWY cyanate degradation
PathBank:SMP0000778 Nitrogen Metabolism
PathBank:SMP0002111 Cyanate Degradation
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-1237044 Erythrocytes take up carbon dioxide and release oxygen
Reactome:R-CEL-1237044 Erythrocytes take up carbon dioxide and release oxygen
Reactome:R-DRE-382551 Transport of small molecules
Reactome:R-DRE-1247673 Erythrocytes take up oxygen and release carbon dioxide
Reactome:R-GGA-1237044 Erythrocytes take up carbon dioxide and release oxygen
Reactome:R-HSA-1480926 O2/CO2 exchange in erythrocytes
Reactome:R-HSA-1234158 Regulation of gene expression by Hypoxia-inducible Factor
Reactome:R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-1480926 O2/CO2 exchange in erythrocytes
Reactome:R-RNO-382551 Transport of small molecules
Reactome:R-RNO-1247673 Erythrocytes take up oxygen and release carbon dioxide
Reactome:R-BTA-1480926 O2/CO2 exchange in erythrocytes
Reactome:R-CEL-382551 Transport of small molecules
Reactome:R-CEL-1480926 O2/CO2 exchange in erythrocytes
Reactome:R-DRE-1237044 Erythrocytes take up carbon dioxide and release oxygen
Reactome:R-DRE-1475029 Reversible hydration of carbon dioxide
Reactome:R-GGA-1480926 O2/CO2 exchange in erythrocytes
Reactome:R-HSA-2262752 Cellular responses to stress
Reactome:R-HSA-449147 Signaling by Interleukins
Reactome:R-HSA-9020591 Interleukin-12 signaling
Reactome:R-RNO-1237044 Erythrocytes take up carbon dioxide and release oxygen
Reactome:R-BTA-1475029 Reversible hydration of carbon dioxide
Reactome:R-BTA-382551 Transport of small molecules
Reactome:R-BTA-1247673 Erythrocytes take up oxygen and release carbon dioxide
Reactome:R-CEL-1247673 Erythrocytes take up oxygen and release carbon dioxide
Reactome:R-DRE-1430728 Metabolism
Reactome:R-GGA-1430728 Metabolism
Reactome:R-GGA-382551 Transport of small molecules
Reactome:R-GGA-1247673 Erythrocytes take up oxygen and release carbon dioxide
Reactome:R-HSA-1237044 Erythrocytes take up carbon dioxide and release oxygen
Reactome:R-HSA-1234174 Cellular response to hypoxia
Reactome:R-HSA-168256 Immune System
Reactome:R-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1475029 Reversible hydration of carbon dioxide
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-1475029 Reversible hydration of carbon dioxide
Reactome:R-DRE-1480926 O2/CO2 exchange in erythrocytes
Reactome:R-GGA-1475029 Reversible hydration of carbon dioxide
Reactome:R-HSA-382551 Transport of small molecules
Reactome:R-HSA-1247673 Erythrocytes take up oxygen and release carbon dioxide
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1475029 Reversible hydration of carbon dioxide
Reactome:R-HSA-8953897 Cellular responses to stimuli
Reactome:R-HSA-1280215 Cytokine Signaling in Immune system
Reactome:R-HSA-447115 Interleukin-12 family signaling
Reactome:R-MMU-1475029 Reversible hydration of carbon dioxide
Reactome:R-MMU-382551 Transport of small molecules
Reactome:R-MMU-1247673 Erythrocytes take up oxygen and release carbon dioxide
Reactome:R-RNO-1480926 O2/CO2 exchange in erythrocytes
WikiPathways:WP5314 Lactate shuttle in glial cells
PathBank:SMP0119398 Gastric Acid Production
PathBank:SMP0121001 Kidney Function- Proximal Convoluted Tubule
PathBank:SMP0000226 Omeprazole Action Pathway
PathBank:SMP0000230 Ranitidine Action Pathway
PathBank:SMP0000246 Pirenzepine Action Pathway
PathBank:SMP0000589 Gastric Acid Production
PathBank:SMP0000734 Roxatidine Acetate Action Pathway
PathBank:SMP0119381 Gastric Acid Production
PathBank:SMP0121011 Kidney Function - Collecting Duct
PathBank:SMP0000225 Esomeprazole Action Pathway
PathBank:SMP0000229 Rabeprazole Action Pathway
PathBank:SMP0000233 Nizatidine Action Pathway
PathBank:SMP0000227 Lansoprazole Action Pathway
PathBank:SMP0000231 Famotidine Action Pathway
PathBank:SMP0000735 Metiamide Action Pathway
PathBank:SMP0000228 Pantoprazole Action Pathway
PathBank:SMP0000232 Cimetidine Action Pathway
PathBank:SMP0000736 Betazole Action Pathway
PathBank:SMP0051038 Lafutidine H2-Antihistamine Action