EC: 4.1.3.39

4-hydroxy-2-oxovalerate aldolase (4-hydroxy-2-oxopentanoate pyruvate-lyase (acetaldehyde-forming))

enzyme lambda metabolic reaction experiment
uniprot:P51015 [Mn2+]

Acetaldehyde + Pyruvate -> 4_Hydroxy_2_oxopentanoate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

Acetaldehyde + Pyruvate -> 4_Hydroxy_2_oxopentanoate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

Propanal + Pyruvate -> 4_Hydroxy_2_oxohexanoate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

Glycolaldehyde + Pyruvate -> 2_Dehydro_3_deoxy_D_xylonate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

Butanal + Pyruvate -> 4_Hydroxy_2_oxoheptanoate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

Pyruvate + 2_Methylpropanal -> 4_Hydroxy_2_oxo_5_methylhexanoate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

Pyruvate + Pentanal -> 4_Hydroxy_2_oxooctanoate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:Q53WH9 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:Q53WI0 [Co2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:Q53WI0 [Co2+]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:Q53WI0 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q53WI0 [Co2+]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q53WI0 [Co2+]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q53WI0 [Mg2+]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q53WI0 [Co2+]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q53WI0 [Ca2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q53WI0 [Ni2+]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
- [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:Q53WH9 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
- [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q53WI0 [Co2+]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ ml L-Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

Oxaloacetate -> Pyruvate + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ ml L-Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

4_Hydroxy_2_oxohexanoate -> Propanal + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ ml L-Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

4_Hydroxy_2_oxoheptanoate -> Pyruvate + Butanal		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ ml L-Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Coenzyme A]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Kic))) ) + S)) buffer: 100 mM HEPES, 0.4 mM NADH, 19.2 U/ ml L-Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Acetaldehyde + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25
uniprot:P51015 [Mn2+]

4_Hydroxy_2_oxopentanoate -> Pyruvate + Acetaldehyde		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-5266 p-cymene degradation
BioCyc:META_PWY-6954 superpathway of aromatic compound degradation via 2-hydroxypentadienoate
BioCyc:META_PWY-5420 catechol degradation II (meta-cleavage pathway)
BioCyc:META_PWY-5178 toluene degradation IV (aerobic) (via catechol)
BioCyc:META_PWY-6957 mandelate degradation to acetyl-CoA
BioCyc:META_PWY-5183 superpathway of aerobic toluene degradation
BioCyc:ECO_PWY-5162 2-hydroxypenta-2,4-dienoate degradation
BioCyc:ECOL316407_PWY0-1277 3-phenylpropionate and 3-(3-hydroxyphenyl)propionate degradation
BioCyc:META_PWY-5273 p-cumate degradation
BioCyc:META_PWY0-1277 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation
BioCyc:META_PWY-5162 2-hydroxypenta-2,4-dienoate degradation
BioCyc:META_PWY-5415 catechol degradation I (meta-cleavage pathway)
BioCyc:META_PWY-6937 superpathway of testosterone and androsterone degradation
BioCyc:ECOO157_PWY-5162 2-oxopentenoate degradation
BioCyc:META_PWY-2504 superpathway of aromatic compound degradation via 3-oxoadipate
BioCyc:META_PWY-5430 meta cleavage pathway of aromatic compounds
BioCyc:META_PWY-6944 androstenedione degradation
BioCyc:META_PWY-5179 toluene degradation V (aerobic) (via toluene-cis-diol)
BioCyc:MTBH37RV_PWY-6947 superpathway of cholesterol degradation II (cholesterol dehydrogenase)
BioCyc:META_PWY-6947 superpathway of cholesterol degradation II (cholesterol dehydrogenase)
BioCyc:META_PWY-6928 superpathway of cholesterol degradation I (cholesterol oxidase)
BioCyc:ECO_PWY0-1277 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation
BioCyc:MTBH37RV_PWY-6944 androstenedione degradation
BioCyc:ECOO157_PWY0-1277 3-phenylpropionate and 3-(3-hydroxyphenyl)propionate degradation
BioCyc:ECOL316407_PWY-5162 2-oxopentenoate degradation
PathBank:SMP0001904 2-Oxopent-4-enoate Metabolism
PathBank:SMP0002049 2-Oxopent-4-enoate Metabolism 2