EC: 4.1.3.24

malyl-CoA lyase ((S)-malyl-CoA glyoxylate-lyase (acetyl-CoA-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q3J5L6 [malyl-CoA lyase(Enzyme) wildtype His-tagged]

Acetyl_CoA + Glyoxylate -> L_Malyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM MOPS-KOH, 10 mM MnCl2
PH: 7.5
Temperature: 30
uniprot:Q3J5L6 [malyl-CoA lyase(Enzyme) wildtype His-tagged]

L_erythro_3_Methylmalyl_CoA -> Glyoxylate + Propanoyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM MOPS-KOH, 10 mM MnCl2, 3.5 mM Phenylhydrazinium chloride
PH: 7.5
Temperature: 30
uniprot:Q3J5L6 [malyl-CoA lyase(Enzyme) wildtype His-tagged]

Glyoxylate + Propanoyl_CoA -> L_erythro_3_Methylmalyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM MOPS-KOH, 10 mM MnCl2
PH: 7.5
Temperature: 30
uniprot:Q3J5L6 [malyl-CoA lyase(Enzyme) wildtype His-tagged]

L_Malyl_CoA -> Glyoxylate + Acetyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM MOPS-KOH, 10 mM MnCl2, 3.5 mM Phenylhydrazinium chloride
PH: 7.5
Temperature: 30
uniprot:Q3J5L6 [malyl-CoA lyase(Enzyme) wildtype His-tagged]

Glyoxylate + Acetyl_CoA -> L_Malyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM MOPS-KOH, 10 mM MnCl2
PH: 7.5
Temperature: 30
uniprot:Q3J5L6 [malyl-CoA lyase(Enzyme) wildtype His-tagged]

Propanoyl_CoA + Glyoxylate -> L_erythro_3_Methylmalyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM MOPS-KOH, 10 mM MnCl2
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6728 methylaspartate cycle
BioCyc:META_PWY-5744 glyoxylate assimilation
BioCyc:META_PWY-5741 ethylmalonyl-CoA pathway
BioCyc:META_PWY-7024 superpathway of the 3-hydroxypropanoate cycle
BioCyc:META_PWY-5743 3-hydroxypropanoate cycle
BioCyc:META_PWY-7854 crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered)