EC: 4.1.1.7

benzoylformate decarboxylase (benzoylformate carboxy-lyase (benzaldehyde-forming))

enzyme lambda metabolic reaction experiment
uniprot:P20906 [Phthalate]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (V * A) ) / (( (K * (1 + (I / Ki))) ) + A)) buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid
PH: 6.1
Temperature: 25
uniprot:P20906 [Phenylacetic acid]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (V * A) ) / (( (K * (1 + (I / Ki))) ) + A)) buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid
PH: 6.1
Temperature: 25
uniprot:P20906 [trans-Cinnamate]

alpha_Oxo_benzeneacetic_acid -> CO2 + Benzaldehyde
(( (V * A) ) / (( (K * (1 + (I / Ki))) ) + A)) buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid
PH: 6.1
Temperature: 25
uniprot:P20906 [(S)-Mandelate]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (V * A) ) / (( (K * (1 + (I / Ki))) ) + A)) buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid
PH: 6.1
Temperature: 25
uniprot:P20906 [(S)-Mandelate]

alpha_Oxo_benzeneacetic_acid -> CO2 + Benzaldehyde
(( (V * A) ) / (( (K * (1 + (I / Ki))) ) + A)) buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid
PH: 6.1
Temperature: 25
uniprot:P20906 [2-Carboxybenzaldehyde]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (V * A) ) / (( (K * (1 + (I / Ki))) ) + A)) buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid
PH: 6.1
Temperature: 25
uniprot:P20906 [Phenoxyacetate]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (V * A) ) / (( (K * (1 + (I / Ki))) ) + A)) buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid
PH: 6.1
Temperature: 25
uniprot:P20906 [Phthalimide]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (V * A) ) / (( (K * (1 + (I / Ki))) ) + A)) buffer: 100 mM 2-(N-morpholino)ethanesulfonic acid
PH: 6.1
Temperature: 25
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) wildtype]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 26 mM Piperazine, 50 mM Gly-Gly, 0.1-0.5 unit/ml Horse liver alcohol dehydrogenase, 0.5 mM Thiamine diphosphate, 0.2 mM NADH
PH: 6.8
Temperature: 20
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant H70A]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 26 mM Piperazine, 50 mM Gly-Gly, 0.1-0.5 unit/ml Horse liver alcohol dehydrogenase, 0.5 mM Thiamine diphosphate, 0.2 mM NADH
PH: 6.8
Temperature: 20
uniprot:P20906 [(R)-Mandelate]

alpha_Oxo_benzeneacetic_acid -> CO2 + Benzaldehyde
(( (( (E * kcat) ) * substrate) ) / (( (Km * (1 + (Inhibitor / Ki))) ) + substrate)) buffer: 26 mM Piperazine, 50 mM Gly-Gly, 0.1-0.5 unit/ml Horse liver alcohol dehydrogenase, 0.5 mM Thiamine diphosphate, 0.2 mM NADH
PH: 6.5
Temperature: 20
uniprot:P20906 [(R)-Mandelate]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (E * kcat) ) * substrate) ) / (( (Km * (1 + (Inhibitor / Ki))) ) + substrate)) buffer: 26 mM Piperazine, 50 mM Gly-Gly, 0.1-0.5 unit/ml Horse liver alcohol dehydrogenase, 0.5 mM Thiamine diphosphate, 0.2 mM NADH
PH: 6.5
Temperature: 20
uniprot:P20906 [(R)-Mandelate]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (E * kcat) ) * substrate) ) / (( (Km * (1 + (Inhibitor / Ki))) ) + substrate)) buffer: 26 mM Piperazine, 50 mM Gly-Gly, 0.1-0.5 unit/ml Horse liver alcohol dehydrogenase, 0.5 mM Thiamine diphosphate, 0.2 mM NADH
PH: 6.5
Temperature: 20
uniprot:P20906 [(R)-Mandelate]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (E * kcat) ) * substrate) ) / (( (Km * (1 + (Inhibitor / Ki))) ) + substrate)) buffer: 26 mM Piperazine, 50 mM Gly-Gly, 0.1-0.5 unit/ml Horse liver alcohol dehydrogenase, 0.5 mM Thiamine diphosphate, 0.2 mM NADH
PH: 6.5
Temperature: 20
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant H281A]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 26 mM Piperazine, 50 mM Gly-Gly, 0.1-0.5 unit/ml Horse liver alcohol dehydrogenase, 0.5 mM Thiamine diphosphate, 0.2 mM NADH
PH: 6.8
Temperature: 20
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant S26A]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 26 mM Piperazine, 50 mM Gly-Gly, 0.1-0.5 unit/ml Horse liver alcohol dehydrogenase, 0.5 mM Thiamine diphosphate, 0.2 mM NADH
PH: 6.8
Temperature: 20
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) wildtype]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant A460I]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant F464I]

alpha_Oxo_benzeneacetic_acid -> CO2 + Benzaldehyde
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant A460I/F464I]

alpha_Oxo_benzeneacetic_acid -> Benzaldehyde + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant A460I]

Pyruvate -> Acetaldehyde + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) wildtype]

Pyruvate -> CO2 + Acetaldehyde
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) wildtype]

2_Oxopentanoate -> Butanal + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant A460I]

2_Oxopentanoate -> CO2 + Butanal
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant A460I]

2_Oxobutyrate -> Propanal + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) wildtype]

2_Oxobutyrate -> Propanal + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant A460I]

2_Oxohexanoate -> CO2 + Pentanal
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) wildtype]

2_Oxohexanoate -> Pentanal + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant A460I]

2_Keto_4_methylhexanoic_acid -> 3_Methylpentanal + CO2
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) mutant A460I]

2_Keto_4_methylpentanoic_acid -> CO2 + 3_Methylbutanal
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) wildtype]

2_Keto_4_methylpentanoic_acid -> CO2 + 3_Methylbutanal
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30
uniprot:P20906 [benzoylformate decarboxylase(Enzyme) wildtype]

2_Keto_4_methylhexanoic_acid -> CO2 + 3_Methylpentanal
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 2.5 mM MgSO4, 0.1 mM ThDP, 0.35 mM NADH, 5 U/ml alcohol dehydrogenase from horse liver
PH: 6
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6954 superpathway of aromatic compound degradation via 2-hydroxypentadienoate
BioCyc:META_PWY-6957 mandelate degradation to acetyl-CoA
BioCyc:META_4-HYDROXYMANDELATE-DEGRADATION-PWY 4-hydroxymandelate degradation
BioCyc:META_PWY-2504 superpathway of aromatic compound degradation via 3-oxoadipate
BioCyc:META_PWY-1501 mandelate degradation I