EC: 4.1.1.31

phosphoenolpyruvate carboxylase (phosphate:oxaloacetate carboxy-lyase (adding phosphate; phosphoenolpyruvate-forming))

enzyme lambda metabolic reaction experiment
- [Mg2+]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.2 mM NADH, 15 µg/ml Malate dehydrogenase; decarbonated water
PH: 8
Temperature: 25
- [Mg2+]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.2 mM NADH, 15 µg/ml Malate dehydrogenase; decarbonated water
PH: 8
Temperature: 25
- [Mg2+]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.2 mM NADH, 15 µg/ml Malate dehydrogenase
PH: 8
Temperature: 25
- [Mg2+]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.2 mM NADH, 15 µg/ml Malate dehydrogenase
PH: 8
Temperature: 25
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (( (S ^ h) ) * (( (n * S) ) / Kis)))) buffer: 100 mM HEPES-KOH, 0.1 mM EDTA, 0.2 mM NADH, 4 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (( (S ^ h) ) * (( (n * S) ) / Kis)))) buffer: 100 mM HEPES-KOH, 0.1 mM EDTA, 0.2 mM NADH, 4 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + Phosphoenolpyruvate + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (( (S ^ h) ) * (( (n * S) ) / Kis)))) buffer: 100 mM HEPES-KOH, 0.1 mM EDTA, 0.2 mM NADH, 4 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (( (S ^ h) ) * (( (n * S) ) / Kis)))) buffer: 100 mM HEPES-KOH, 0.1 mM EDTA, 0.2 mM NADH, 4 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Mg2+]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (( (S ^ h) ) * (( (n * S) ) / Kis)))) buffer: 100 mM HEPES-KOH, 0.1 mM EDTA, 0.2 mM NADH, 4 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Mg2+]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (( (S ^ h) ) * (( (n * S) ) / Kis)))) buffer: 100 mM HEPES-KOH, 0.1 mM EDTA, 0.2 mM NADH, 4 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Mg2+]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM HEPES-KOH, 1 mM EDTA, 0.2 mM NADH, 8 units/ml malate dehydrogenase
PH: 7.3
Temperature: 30
- [Mg2+]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.2 mM NADH, 15 µg/ml Malate dehydrogenase
PH: 8
Temperature: 25
- [Mg2+]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.2 mM NADH, 15 µg/ml Malate dehydrogenase
PH: 8
Temperature: 25
uniprot:P04711 [Glucose 6-phosphate]

CO2 + Phosphoenolpyruvate + H2O -> Oxaloacetate + Phosphate		 (( (( (( (Vamax - Vo) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) ) + Vo) buffer: 100 mM HEPES-KOH, 1 mM EDTA, 0.2 mM NADH, 8 units/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

CO2 + Phosphoenolpyruvate + H2O -> Oxaloacetate + Phosphate		 (( (( (( (Vamax - Vo) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) ) + Vo) buffer: 100 mM HEPES-KOH, 1 mM EDTA, 0.2 mM NADH, 8 units/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (( (( (Vamax - Vo) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) ) + Vo) buffer: 100 mM HEPES-KOH, 1 mM EDTA, 0.2 mM NADH, 8 units/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (( (( (Vamax - Vo) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) ) + Vo) buffer: 100 mM HEPES-KOH, 1 mM EDTA, 0.2 mM NADH, 8 units/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Malate]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vo * I_50) ) / (I_50 + I)) buffer: 100 mM HEPES-KOH, 1 mM EDTA, 0.2 mM NADH, 8 units/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Malate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vo * I_50) ) / (I_50 + I)) buffer: 100 mM HEPES-KOH, 1 mM EDTA, 0.2 mM NADH, 8 units/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Mg2+]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM HEPES-KOH, 1 mM EDTA, 0.2 mM NADH, 8 units/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Mg2+]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (( (Vmax * (( (( (S * M) ) * K_1) ) ^ h)) ) * (1 + (S / (alpha * Ka_PEP)))) ) / (( (( (K_MgPEP ^ h) ) * (( (( (1 + (M / Ki_Mg)) ) + (S / Ka_PEP)) ) + (( (S * M) ) / (Ka_PEP * Ki_Mg)))) ) + (( (( (( (S * M) ) * K_1) ) ^ h) ) * (( (( (1 + (S / (alpha * Ka_PEP))) ) + (M / Ki_Mg)) ) + (( (S * M) ) / (Ka_PEP * Ki_Mg)))))) buffer: 100 mM HEPES-KOH, 0.1 mM EDTA, 0.2 mM NADH, 4 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Mg2+]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (( (S ^ h) ) * (( (n * S) ) / Kis)))) buffer: 100 mM HEPES-KOH, 0.1 mM EDTA, 0.2 mM NADH, 4 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R231Q]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R372Q]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R231Q]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R183Q/R184Q]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R184Q]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R183Q/R184Q]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

CO2 + Phosphoenolpyruvate + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

CO2 + Phosphoenolpyruvate + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R372Q]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P29195 [phosphoenolpyruvate carboxylase(Enzyme) mutant G961A]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 2 mM dithiothreitol, 5 units/ml of NADH malate dehydrogenase, 1 mM NADH
PH: 7.3
Temperature: 30
uniprot:P29195 [phosphoenolpyruvate carboxylase(Enzyme) mutant G961V]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 2 mM dithiothreitol, 5 units/ml of NADH malate dehydrogenase, 1 mM NADH
PH: 7.3
Temperature: 30
uniprot:P29195 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 2 mM dithiothreitol, 5 units/ml of NADH malate dehydrogenase, 1 mM NADH
PH: 7.3
Temperature: 30
uniprot:P29195 [phosphoenolpyruvate carboxylase(Enzyme) wildtype delta C1]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 2 mM dithiothreitol, 5 units/ml of NADH malate dehydrogenase, 1 mM NADH
PH: 7.3
Temperature: 30
uniprot:P29195 [phosphoenolpyruvate carboxylase(Enzyme) wildtype delta C4]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 2 mM dithiothreitol, 5 units/ml of NADH malate dehydrogenase, 1 mM NADH
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R184Q]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R183Q]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) mutant R183Q]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (va_max - v0) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (v0 - (( (( (va_max - v0) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (va_max - v0) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (v0 - (( (( (va_max - v0) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (v0 - (( (( (va_max - v0) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [L-Malate]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [L-Malate]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

CO2 + Phosphoenolpyruvate + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (v0 - (( (( (va_max - v0) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.3
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [Glycine]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:P04711 [L-Malate]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (v0 - (( (( (v0 - vi_min) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n)))) buffer: 100 mM Hepes-NaOH, 10 mM KHCO3, 10 mM MgSO4, 0.1 mM NADH, 1.5 U/ml malate dehydrogenase
PH: 7.4
Temperature: 30
uniprot:Q9FV66 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Ticine-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 8
Temperature: 25
uniprot:P30694 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:Q01647 [Glucose 6-phosphate]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Ticine-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q01647 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P30694 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:Q01647 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Ticine-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q01647 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

CO2 + Phosphoenolpyruvate + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:Q9FV66 [Glucose 6-phosphate]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:Q9FV66 [Glucose 6-phosphate]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Ticine-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q9FV65 [Glucose 6-phosphate]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:Q9FV65 [Glucose 6-phosphate]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Ticine-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 8
Temperature: 25
uniprot:P30694 [Glucose 6-phosphate]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Ticine-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q9FV65 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Ticine-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q9FV66 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:Q9FV65 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Hepes-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P30694 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) buffer: 50 mM Ticine-KOH, 10 mM MgCl2, 0.15 mM NADH, 10 U/ml NADH-malate dehydrogenase
PH: 8
Temperature: 25
uniprot:P51059 [Glucose 6-phosphate]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km_A + A)) buffer:
PH: 8.5
Temperature: 36
uniprot:P04711 [Glucose 6-phosphate]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km_A + A)) buffer:
PH: 8.5
Temperature: 36
uniprot:P51059 [phosphoenolpyruvate carboxylase(Enzyme) wildtype PC-II]

CO2 + Phosphoenolpyruvate + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km_A + A)) buffer:
PH: 8.5
Temperature: 36
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) wildtype PC-I]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km_A + A)) buffer:
PH: 8.5
Temperature: 36
uniprot:P51059 [phosphoenolpyruvate carboxylase(Enzyme) wildtype PC-II]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * B) ) / (Km_B + B)) buffer:
PH: 7.9
Temperature: 10
uniprot:P04711 [phosphoenolpyruvate carboxylase(Enzyme) wildtype PC-I]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * B) ) / (Km_B + B)) buffer:
PH: 7.9
Temperature: 10
uniprot:Q9MAH0 [Mg2+]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 15 % (v/v) glycerol, 2 mM DTT, 0.15 mM NADH, 5 U/ml desalted porcine muscle malate dehydrogenase
PH: 7.3
Temperature: 25
uniprot:Q9MAH0 [Mg2+]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 15 % (v/v) glycerol, 2 mM DTT, 0.15 mM NADH, 5 U/ml desalted porcine muscle malate dehydrogenase
PH: 8.5
Temperature: 25
uniprot:Q9MAH0 [Mg2+]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes/KOH, 15 % (v/v) glycerol, 2 mM DTT, 0.15 mM NADH, 5 U/ml desalted porcine muscle malate dehydrogenase
PH: 7.3
Temperature: 25
uniprot:C7DY59 [Isocitrate]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 7.3
Temperature: 24
uniprot:C7DY59 [Glucose 6-phosphate]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 7.3
Temperature: 24
uniprot:C7DY59 [L-Malate]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 7.3
Temperature: 24
uniprot:C7DY59 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 8.4
Temperature: 24
uniprot:C7DY59 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase
PH: 8.4
Temperature: 24
uniprot:C7DY59 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 7.3
Temperature: 24
uniprot:C7DY59 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase
PH: 7.3
Temperature: 24
uniprot:C7DY59 [L-Aspartate]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 7.3
Temperature: 24
uniprot:C7DY59 [L-Glutamate]

Phosphoenolpyruvate + CO2 + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 7.3
Temperature: 24
uniprot:C7DY59 [Mg2+]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 8.4
Temperature: 24
uniprot:C7DY59 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 8.4
Temperature: 24
uniprot:C7DY59 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase
PH: 8.4
Temperature: 24
uniprot:C7DY59 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase, 10% (v/v) glycerol
PH: 7.3
Temperature: 24
uniprot:C7DY59 [phosphoenolpyruvate carboxylase(Enzyme) wildtype]

H2O + CO2 + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM bistrispropane/HCl, 2.5 mM KHCO3, 12 mM MgCl2, 0.15 mM NADH, 5 U/ml porcine heart malate dehydrogenase
PH: 7.3
Temperature: 24
- [Mg2+]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 7.3
Temperature: 30
- [Mg2+]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 7.3
Temperature: 30
- [Mg2+]

CO2 + Phosphoenolpyruvate + H2O -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 7.3
Temperature: 30
- [Mg2+]

CO2 + H2O + Phosphoenolpyruvate -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

Phosphoenolpyruvate + H2O + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 7.3
Temperature: 30
- [Mg2+]

CO2 + H2O + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 7.3
Temperature: 30
- [Mg2+]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

Phosphoenolpyruvate + CO2 + H2O -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 7.3
Temperature: 30
- [Mg2+]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 8
Temperature: 30
- [Mg2+]

H2O + Phosphoenolpyruvate + CO2 -> Phosphate + Oxaloacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 0.2 mM NADH, 4 U/ml Malate dehydrogenase
PH: 8
Temperature: 30
uniprot:O27026 [Mg2+]

Phosphoenolpyruvate + H2O + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.2 mM NADH + H+, 1 U/ml Malate dehydrogenase from Thermus flavus
PH: 8
Temperature: 50
uniprot:O27026 [Mg2+]

H2O + CO2 + Phosphoenolpyruvate -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.2 mM NADH + H+, 1 U/ml Malate dehydrogenase from Thermus flavus
PH: 8
Temperature: 50
- [Mg2+]

H2O + Phosphoenolpyruvate + CO2 -> Oxaloacetate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-H2SO4, 0.15 mM NADH, 2 U/ml Thermus flavas malate dehydrogenase
PH: 8.5
Temperature: 85

Pathways

pathway id name
BioCyc:META_PWYQT-4429 CO2 fixation into oxaloacetate (anaplerotic)
BioCyc:META_PWY-6549 L-glutamine biosynthesis III
BioCyc:ECOL199310_ANARESP1-PWY respiration (anaerobic)
BioCyc:CAULO_GLUCONEO-PWY gluconeogenesis I
BioCyc:CAULO_FERMENTATION-PWY mixed acid fermentation
BioCyc:META_PWY-1622 formaldehyde assimilation I (serine pathway)
BioCyc:META_PWY-6142 gluconeogenesis II (Methanobacterium thermoautotrophicum)
BioCyc:ARA_PWY-6549 L-glutamine biosynthesis III
BioCyc:ECOO157_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOL199310_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOL316407_ANARESP1-PWY respiration (anaerobic)
BioCyc:META_PWY-6146 Methanobacterium thermoautotrophicum biosynthetic metabolism
BioCyc:ARA_PWYQT-4429 CO2 fixation into oxaloacetate (anaplerotic)
BioCyc:ECOO157_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOL199310_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOL316407_FERMENTATION-PWY mixed acid fermentation
BioCyc:CORYNE_FERMENTATION-PWY mixed acid fermentation
BioCyc:META_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECO_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOO157_ANARESP1-PWY respiration (anaerobic)
BioCyc:CAULO_ANARESP1-PWY respiration (anaerobic)
BioCyc:CAULO_PWYQT-4429 CO2 fixation into oxaloacetate (anapleurotic)
PlantCyc:ARA_PWYQT-4429 CO2 fixation into oxaloacetate (anaplerotic)
PlantCyc:PLANT_PWY-6549 L-glutamine biosynthesis III
PlantCyc:PLANT_PWYQT-4429 CO2 fixation into oxaloacetate (anaplerotic)
PlantCyc:ARA_PWY-6549 L-glutamine biosynthesis III
WikiPathways:WP2621 Glycolysis
PathBank:SMP0002426 Glutamine Metabolism