EC: 3.6.5.4

signal-recognition-particle GTPase (GTP phosphohydrolase (protein-synthesis-assisting))

enzyme lambda metabolic reaction experiment
uniprot:P08411 [signal-recognition-particle GTPase(Enzyme) wildtype Nsp2 His6-tagged]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 150 mM NaCl, 2 mM DTT
PH: 7.5
Temperature: 37
uniprot:P9WGD9 [Mg2+]

H2O + ATP -> ADP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mg2+]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mg2+]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mg2+]

GTP + H2O -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant R359G]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:P9WGD9 [Mg2+]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mg2+]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mg2+]

H2O + GTP -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mg2+]

H2O + GTP -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mg2+]

H2O + GTP -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) wildtype]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant G147E]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant G192D]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant R196Q]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant R98G]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant G188R]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant Y207C]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant D292V]

H2O + GTP -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant V220M]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant G274S]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant E250K]

GTP + H2O -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant K276E]

GTP + H2O -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant T243N]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant R209S]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:Q8IVH4 [signal-recognition-particle GTPase(Enzyme) mutant G218E]

GTP + H2O -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM MgCl2, 0.1 mM sodium azide
PH: 7.5
Temperature: 22
uniprot:P9WGD9 [Glycerol]

GTP + H2O -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Glycerol]

GTP + H2O -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Glycerol]

H2O + GTP -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mn2+]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mn2+]

GTP + H2O -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mn2+]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mn2+]

H2O + GTP -> Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mn2+]

H2O + GTP -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P9WGD9 [Mg2+]

GTP + H2O -> GDP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 1 mM DTT
PH: 8
Temperature: 37

Pathways

pathway id name
WikiPathways:WP2472 Escherichia coli K-12 peripherome
Reactome:R-RNO-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-BTA-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-CFA-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-MMU-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-DRE-392499 Metabolism of proteins
Reactome:R-DDI-72766 Translation
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-RNO-72766 Translation
Reactome:R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-DRE-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-SCE-72766 Translation
Reactome:R-SPO-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-BTA-392499 Metabolism of proteins
Reactome:R-CFA-392499 Metabolism of proteins
Reactome:R-DRE-72766 Translation
Reactome:R-DDI-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-HSA-72766 Translation
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-BTA-72766 Translation
Reactome:R-CFA-72766 Translation
Reactome:R-MMU-72766 Translation
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SPO-72766 Translation