EC: 3.6.1.56

2-hydroxy-dATP diphosphatase (2-hydroxy-dATP diphosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P36639 [2-hydroxy-dATP diphosphatase(Enzyme) wildtype]

H2O + dGTP -> dGMP + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 4 mM MgCl2, 40 mM NaCl, 80 µg/ml bovine serum albumin, 8 mM dithiothreitol, 10% glycerol
PH: 8
Temperature: 30
uniprot:P36639 [2-hydroxy-dATP diphosphatase(Enzyme) wildtype]

8_oxo_dGTP + H2O -> Diphosphate + 8_oxo_dGMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: PPiLight™ Inorganic Pyrophosphate Assay (Lonza Rockland Inc.)
PH: 7.5
Temperature: 22
uniprot:P36639 [2-hydroxy-dATP diphosphatase(Enzyme) wildtype]

2_hydroxy_dATP + H2O -> 2_hydroxy_dAMP + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 4 mM MgCl2, 40 mM NaCl, 80 µg/ml bovine serum albumin, 8 mM dithiothreitol, 10% glycerol
PH: 8
Temperature: 30
uniprot:P36639 [2-hydroxy-dATP diphosphatase(Enzyme) wildtype]

2_hydroxy_dATP + H2O -> Diphosphate + 2_hydroxy_dAMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 4 mM MgCl2, 40 mM NaCl, 80 µg/ml bovine serum albumin, 8 mM dithiothreitol, 10% glycerol
PH: 7.2
Temperature: 30
uniprot:P36639 [2-hydroxy-dATP diphosphatase(Enzyme) wildtype]

8_hydroxy_dATP + H2O -> Diphosphate + 8_hydroxy_dAMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 4 mM MgCl2, 40 mM NaCl, 80 µg/ml bovine serum albumin, 8 mM dithiothreitol, 10% glycerol
PH: 8
Temperature: 30
uniprot:P36639 [2-hydroxy-dATP diphosphatase(Enzyme) wildtype]

8_hydroxy_dGTP + H2O -> Diphosphate + 8_hydroxy_dGMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 4 mM MgCl2, 40 mM NaCl, 80 µg/ml bovine serum albumin, 8 mM dithiothreitol, 10% glycerol
PH: 7.2
Temperature: 30
uniprot:P36639 [2-hydroxy-dATP diphosphatase(Enzyme) wildtype]

H2O + 8_hydroxy_dGTP -> Diphosphate + 8_hydroxy_dGMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 4 mM MgCl2, 40 mM NaCl, 80 µg/ml bovine serum albumin, 8 mM dithiothreitol, 10% glycerol
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6502 oxidized GTP and dGTP detoxification
BioCyc:HUMAN_PWY-6502 oxidized GTP and dGTP detoxification
Reactome:R-CFA-1430728 Metabolism
Reactome:R-DRE-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-RNO-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-CFA-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-DRE-74259 Purine catabolism
Reactome:R-MMU-74259 Purine catabolism
Reactome:R-RNO-74259 Purine catabolism
Reactome:R-CFA-15869 Nucleotide metabolism
Reactome:R-CFA-8956319 Nucleotide catabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-CFA-74259 Purine catabolism
Reactome:R-DRE-15869 Nucleotide metabolism
Reactome:R-DRE-8956319 Nucleotide catabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-8956319 Nucleotide catabolism
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-8956319 Nucleotide catabolism