uniprot:Q12524 [8-oxo-dGTP diphosphatase(Enzyme) wildtype]
Coenzyme_A + H2O -> Adenosine_3',5'_bisphosphate + 4'_Phosphopantetheine |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane-HCl, 5 mM MgCl2, 1 mM DTT
PH: 7
Temperature: 37 |
uniprot:Q12524 [8-oxo-dGTP diphosphatase(Enzyme) wildtype]
CoA_disulfide + H2O -> Adenosine_3',5'_bisphosphate + CoA_4'_Phosphopantetheine_disulfide |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane-HCl, 5 mM MgCl2, 1 mM DTT
PH: 7
Temperature: 37 |
uniprot:P08337 [Mg2+]
H2O + dTTP -> dTMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
dCTP + H2O -> dCMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
H2O + dGTP -> dGMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
H2O + 8_oxo_dGTP -> 8_oxo_dGMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
8_oxo_dGDP + H2O -> 8_oxo_dGMP + Phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
dTDP + H2O -> Phosphate + dTMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
GDP + H2O -> Phosphate + GMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
GTP + H2O -> GMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
H2O + dCDP -> dCMP + Phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
H2O + dATP -> dAMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
8_oxo_GDP + H2O -> 8_oxo_GMP + Phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
dADP + H2O -> dAMP + Phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
H2O + dGDP -> dGMP + Phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |
uniprot:P08337 [Mg2+]
8_oxo_GTP + H2O -> Diphosphate + 8_oxo_GMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml BSA, 40 mM NaCl, 5 mM DTT, 2% glycerol
PH: 8
Temperature: 30 |