EC: 3.6.1.15

nucleoside-triphosphate phosphatase (unspecific diphosphate phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q9BSD7 [nucleoside-triphosphatase(Enzyme) wildtype]

ADP + H2O -> AMP + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Mops, 10 mM MgCl2, 5 mM DTT, 200 µM MESG, 0.2 U/ml PNP
PH: 7
Temperature: 25
uniprot:Q9BSD7 [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + ATP -> Phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Mops, 10 mM MgCl2, 5 mM DTT, 200 µM MESG, 0.2 U/ml PNP
PH: 7
Temperature: 25
uniprot:Q9BSD7 [nucleoside-triphosphatase(Enzyme) wildtype]

GTP + H2O -> Phosphate + GDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Mops, 10 mM MgCl2, 5 mM DTT, 200 µM MESG, 0.2 U/ml PNP
PH: 7
Temperature: 25
uniprot:Q9BSD7 [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + CTP -> CDP + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Mops, 10 mM MgCl2, 5 mM DTT, 200 µM MESG, 0.2 U/ml PNP
PH: 7
Temperature: 25
uniprot:Q9BSD7 [nucleoside-triphosphatase(Enzyme) wildtype]

UTP + H2O -> Phosphate + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Mops, 10 mM MgCl2, 5 mM DTT, 200 µM MESG, 0.2 U/ml PNP
PH: 7
Temperature: 25
uniprot:Q9BSD7 [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + dTTP -> TDP + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Mops, 10 mM MgCl2, 5 mM DTT, 200 µM MESG, 0.2 U/ml PNP
PH: 7
Temperature: 25
uniprot:P11125 [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + ATP -> Phosphate + ADP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 75 mM NaCl, 7.5 mM MgCl2
PH: 8
Temperature: 25
uniprot:P11125 [nucleoside-triphosphatase(Enzyme) wildtype]

UTP + H2O -> Phosphate + UDP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 75 mM NaCl, 7.5 mM MgCl2
PH: 8
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + ATP -> Phosphate + ADP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 75 mM NaCl, 7.5 mM MgCl2
PH: 8
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + ATP -> Phosphate + ADP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 75 mM NaCl, 7.5 mM MgCl2
PH: 8
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

UTP + H2O -> UDP + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 75 mM NaCl, 7.5 mM MgCl2
PH: 8
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + UTP -> Phosphate + UDP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 75 mM NaCl, 7.5 mM MgCl2
PH: 8
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + ATP -> ADP + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 25 µg/ml poly(U)
PH: 6.6
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + CTP -> CDP + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 25 µg/ml poly(U)
PH: 6.6
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + GTP -> Phosphate + GDP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 25 µg/ml poly(U)
PH: 6.6
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + UTP -> UDP + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 25 µg/ml poly(U)
PH: 6.6
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

dUTP + H2O -> Phosphate + dUDP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 25 µg/ml poly(U)
PH: 6.6
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + dATP -> Phosphate + dADP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 25 µg/ml poly(U)
PH: 6.6
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

H2O + dCTP -> Phosphate + dCDP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 25 µg/ml poly(U)
PH: 6.6
Temperature: 25
- [nucleoside-triphosphatase(Enzyme) wildtype]

dGTP + H2O -> dGDP + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 25 µg/ml poly(U)
PH: 6.6
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:META_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:META_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:META_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:HUMAN_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:PLANT_PWY-5687 pyrimidine ribonucleotides interconversion
PlantCyc:PLANT_PWY-7185 UTP and CTP dephosphorylation I
PlantCyc:PLANT_PWY-7177 UTP and CTP dephosphorylation II
Reactome:R-HSA-9679506 SARS-CoV Infections
Reactome:R-HSA-9694516 SARS-CoV-2 Infection
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9705683 SARS-CoV-2-host interactions
Reactome:R-HSA-9754678 SARS-CoV-2 modulates host translation machinery
Reactome:R-HSA-9918432 Maturation of DENV proteins
Reactome:R-HSA-9920951 Dengue virus modulates apoptosis
Reactome:R-HSA-9918487 Dengue Virus Genome Translation and Replication
Reactome:R-HSA-9920588 Dengue virus activates/modulates innate and adaptive immune responses
Reactome:R-HSA-9839923 Dengue Virus Infection
Reactome:R-HSA-9918476 Assembly and Release of Dengue Virus Virions
Reactome:R-HSA-9918481 Dengue Virus-Host Interactions
Reactome:R-DRE-191859 snRNP Assembly
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-8953854 Metabolism of RNA
Reactome:R-XTR-15869 Nucleotide metabolism
Reactome:R-XTR-8956319 Nucleotide catabolism
Reactome:R-XTR-8850843 Phosphate bond hydrolysis by NTPDase proteins
Reactome:R-DRE-8953854 Metabolism of RNA
Reactome:R-DRE-194441 Non-coding RNA Metabolism
Reactome:R-HSA-199991 Membrane Trafficking
Reactome:R-HSA-8854214 TBC/RABGAPs
Reactome:R-HSA-191859 snRNP Assembly
Reactome:R-HSA-9007101 Rab regulation of trafficking
Reactome:R-HSA-8953854 Metabolism of RNA
Reactome:R-MMU-194441 Non-coding RNA Metabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-HSA-5653656 Vesicle-mediated transport
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-8956319 Nucleotide catabolism
Reactome:R-HSA-8850843 Phosphate bond hydrolysis by NTPDase proteins
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-HSA-194441 Non-coding RNA Metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-8956319 Nucleotide catabolism
Reactome:R-MMU-8850843 Phosphate bond hydrolysis by NTPDase proteins
Reactome:R-MMU-191859 snRNP Assembly
PathBank:SMP0087212 Thiamine Metabolism
PathBank:SMP0000076 Thiamine Metabolism
PathBank:SMP0063677 Thiamine Metabolism