EC: 3.5.5.4

cyanoalanine nitrilase (3-cyano-L-alanine aminohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q3LRV4 [cyanoalanine nitrilase(Enzyme) wildtype LaNIT4B His6-tagged]

3_Cyano_L_alanine + 2 H2O -> NH3 + L_Aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 1 mM DTT
PH: 8
Temperature: 30
uniprot:Q5QGZ8 [cyanoalanine nitrilase(Enzyme) wildtype LaNIT4A His6-tagged]

2 H2O + 3_Cyano_L_alanine -> NH3 + L_Aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 1 mM DTT
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_ASPSYNII-PWY cyanide detoxification I