EC: 3.5.4.4

adenosine deaminase (adenosine aminohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:O25046 [adenosine deaminase(Enzyme) wildtype]

Adenosine + H2O -> NH3 + Inosine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 0.15 mM NADH, 25 mM R-ketoglutarate, 5 µg/ml Glutamate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:O25046 [adenosine deaminase(Enzyme) wildtype His6-tagged]

H2O + Adenosine -> NH3 + Inosine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:P03958 [adenosine deaminase(Enzyme) mutant E217D]

Adenosine + H2O -> Inosine + NH3		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 7.2
Temperature: 25
uniprot:P03958 [adenosine deaminase(Enzyme) wildtype]

Adenosine + H2O -> Inosine + NH3		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 7.2
Temperature: 25
uniprot:P03958 [adenosine deaminase(Enzyme) mutant E217Q]

H2O + Adenosine -> NH3 + Inosine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 7.2
Temperature: 25
uniprot:P03958 [adenosine deaminase(Enzyme) mutant E217G]

H2O + Adenosine -> NH3 + Inosine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 7.2
Temperature: 25
uniprot:Q9X034 [adenosine deaminase(Enzyme) wildtype]

Adenosine + H2O -> NH3 + Inosine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM HEPES, 7.4 mM alpha-Ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:P00813 [adenosine deaminase(Enzyme) wildtype]

H2O + Adenosine -> Inosine + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 0.4 M sodium acetate
PH: 5.8
Temperature: 37
uniprot:Q8IJA9 [Hypoxanthine]

H2O + Adenosine -> Inosine + NH3		 (( (Vmax * IC50) ) / (IC50 + I)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36
uniprot:Q8IJA9 [adenosine deaminase(Enzyme) wildtype]

Adenosine + H2O -> NH3 + Inosine		 (( (Vmax * IC50) ) / (IC50 + I)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 36

Pathways

pathway id name
BioCyc:HUMAN_PWY-7179-1 purine deoxyribonucleosides degradation
BioCyc:META_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:META_PWY-7179 purine deoxyribonucleosides degradation I
BioCyc:META_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:META_PWY-6609 adenine and adenosine salvage III
BioCyc:ECO_PWY-7179 purine deoxyribonucleosides degradation I
BioCyc:VCHO_PWY-7179 purine deoxyribonucleosides degradation I
BioCyc:ECOO157_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:ECOO157_PWY0-1296 purine ribonucleosides degradation
BioCyc:ECOO157_PWY-7179 purine deoxyribonucleosides degradation
BioCyc:ECOO157_PWY-6609 adenine and adenosine salvage III
BioCyc:ECOL199310_PWY-7179 purine deoxyribonucleosides degradation
BioCyc:ECOL199310_PWY0-1297 superpathway of purine deoxyribonucleosides degradation
BioCyc:MOUSE_PWY0-1296 purine ribonucleosides degradation to ribose-1-phosphate
BioCyc:ECOL316407_PWY-6611 adenine and adenosine salvage V
BioCyc:HUMAN_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:META_PWY0-1296 purine ribonucleosides degradation
BioCyc:VCHO_PWY0-1296 purine ribonucleosides degradation
BioCyc:VCHO_PWY-6611 adenine and adenosine salvage V
BioCyc:VCHO_PWY-6609 adenine and adenosine salvage III
BioCyc:MTBH37RV_PWY0-1296 purine ribonucleosides degradation
BioCyc:MTBH37RV_PWY-7179 purine deoxyribonucleosides degradation I
BioCyc:MTBH37RV_PWY-6609 adenine and adenosine salvage III
BioCyc:HPY_PWY-7179 purine deoxyribonucleosides degradation I
BioCyc:ECOO157_PWY-6611 adenine and adenosine salvage V
BioCyc:ECOL199310_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:ECOL199310_PWY-6609 adenine and adenosine salvage III
BioCyc:ECOL199310_PWY-6611 adenine and adenosine salvage V
BioCyc:MOUSE_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:MOUSE_PWY-6609 adenine and adenosine salvage III
BioCyc:HUMAN_SALVADEHYPOX-PWY adenosine nucleotides degradation
BioCyc:HUMAN_PWY0-1296 purine ribonucleosides degradation to ribose-1-phosphate
BioCyc:META_PWY-6611 adenine and adenosine salvage V
BioCyc:ECO_PWY-6609 adenine and adenosine salvage III
BioCyc:ECO_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:VCHO_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:VCHO_PWY0-1297 superpathway of purine deoxyribonucleosides degradation
BioCyc:HPY_PWY0-1296 purine ribonucleosides degradation
BioCyc:ECOO157_PWY0-1297 superpathway of purine deoxyribonucleosides degradation
BioCyc:ECOL199310_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:MOUSE_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:ECOL316407_PWY-6609 adenine and adenosine salvage III
BioCyc:ECOL316407_PWY0-1296 purine ribonucleosides degradation
BioCyc:HUMAN_PWY-6353 purine nucleotides degradation
BioCyc:HUMAN_PWY-6609 adenine and adenosine salvage III
BioCyc:META_PWY-7179-1 purine deoxyribonucleosides degradation II
BioCyc:META_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:META_PWY0-1297 superpathway of purine deoxyribonucleosides degradation
BioCyc:ECO_PWY-6611 adenine and adenosine salvage V
BioCyc:ECO_PWY0-1296 purine ribonucleosides degradation
BioCyc:ECO_PWY0-1297 superpathway of purine deoxyribonucleosides degradation
BioCyc:MTBH37RV_PWY0-1297 superpathway of purine deoxyribonucleosides degradation
BioCyc:HPY_PWY-6609 adenine and adenosine salvage III
BioCyc:ECOL199310_PWY0-1296 purine ribonucleosides degradation
BioCyc:ECOL316407_SALVADEHYPOX-PWY adenosine nucleotides degradation II
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0002081 Adenine and Adenosine Salvage I
PathBank:SMP0002083 Adenine and Adenosine Salvage II
PathBank:SMP0002088 Purine Ribonucleosides Degradation
PathBank:SMP0002089 Purine Deoxyribonucleosides Degradation
PathBank:SMP0002103 Adenosine Nucleotides Degradation
Reactome:R-RNO-9748784 Drug ADME
Reactome:R-DRE-9755088 Ribavirin ADME
Reactome:R-XTR-9748784 Drug ADME
Reactome:R-MMU-9755088 Ribavirin ADME
Reactome:R-XTR-9755088 Ribavirin ADME
Reactome:R-MMU-9748784 Drug ADME
Reactome:R-PFA-9748784 Drug ADME
Reactome:R-DDI-9755088 Ribavirin ADME
Reactome:R-RNO-9755088 Ribavirin ADME
Reactome:R-DRE-9748784 Drug ADME
Reactome:R-DDI-9748784 Drug ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-HSA-9755088 Ribavirin ADME
Reactome:R-PFA-9755088 Ribavirin ADME
Reactome:R-DRE-74217 Purine salvage
Reactome:R-DRE-392499 Metabolism of proteins
Reactome:R-DDI-5683826 Surfactant metabolism
Reactome:R-DDI-8956321 Nucleotide salvage
Reactome:R-HSA-74217 Purine salvage
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-74217 Purine salvage
Reactome:R-RNO-74217 Purine salvage
Reactome:R-XTR-15869 Nucleotide metabolism
Reactome:R-DRE-8956321 Nucleotide salvage
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-HSA-8956321 Nucleotide salvage
Reactome:R-MMU-8956321 Nucleotide salvage
Reactome:R-PFA-74217 Purine salvage
Reactome:R-RNO-8956321 Nucleotide salvage
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DDI-74217 Purine salvage
Reactome:R-PFA-1430728 Metabolism
Reactome:R-PFA-15869 Nucleotide metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-8956321 Nucleotide salvage
Reactome:R-DRE-15869 Nucleotide metabolism
Reactome:R-DRE-5683826 Surfactant metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-5683826 Surfactant metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-PFA-8956321 Nucleotide salvage
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-XTR-74217 Purine salvage
WikiPathways:WP4792 Purine metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0120670 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0120669 Adenosine Deaminase Deficiency
PathBank:SMP0120673 AICA-Ribosiduria
PathBank:SMP0120709 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120717 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120797 Xanthinuria Type II
PathBank:SMP0120821 Myoadenylate Deaminase Deficiency
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120728 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120752 Molybdenum Cofactor Deficiency
PathBank:SMP0120756 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120796 Xanthinuria Type I
PathBank:SMP0120820 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0087332 Purine Metabolism
PathBank:SMP0120819 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway