EC: 3.5.3.13

formimidoylglutamate deiminase (N-formimidoyl-L-glutamate iminohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant E235D (Zn2+)]

N_Formimino_L_glutamate + H2O -> N_Formyl_L_glutamate + NH3
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) wildtype (Zn2+)]

N_Formimino_L_glutamate + H2O -> N_Formyl_L_glutamate + NH3
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [Cu2+]

H2O + N_Formimino_L_glutamate -> NH3 + N_Formyl_L_glutamate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant E235A (Zn2+)]

N_Formimino_L_glutamate + H2O -> N_Formyl_L_glutamate + NH3
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant E235Q (Zn2+)]

H2O + N_Formimino_L_glutamate -> NH3 + N_Formyl_L_glutamate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [Cd2+]

H2O + N_Formimino_L_glutamate -> NH3 + N_Formyl_L_glutamate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant H269A (Zn2+)]

H2O + N_Formimino_L_glutamate -> N_Formyl_L_glutamate + NH3
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [Ni2+]

H2O + N_Formimino_L_glutamate -> NH3 + N_Formyl_L_glutamate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) wildtype (Zn2+)]

H2O + N_Formimino_L_glutamate -> N_Formyl_L_glutamate + NH3
(( (E * C) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [Cu2+]

N_Formimino_L_glutamate + H2O -> N_Formyl_L_glutamate + NH3
(( (E * kcat) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant D320C (Zn2+)]

H2O + N_Formimino_L_glutamate -> NH3 + N_Formyl_L_glutamate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant D320A (Zn2+)]

H2O + N_Formimino_L_glutamate -> N_Formyl_L_glutamate + NH3
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) wildtype (Zn2+)]

N_Formimino_L_glutamate + H2O -> NH3 + N_Formyl_L_glutamate
(( (E * kcat) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant H269C (Zn2+)]

H2O + N_Formimino_L_glutamate -> NH3 + N_Formyl_L_glutamate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant H269N (Zn2+)]

H2O + N_Formimino_L_glutamate -> N_Formyl_L_glutamate + NH3
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 100 mM KCl, 7.4 mM alpha-ketoglutarate, 0.4 mM NADH, 24 U/ml glutamate dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9HU77 [Cd2+]

H2O + N_Formimino_L_glutamate -> NH3 + N_Formyl_L_glutamate
(( (E * kcat) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [Ni2+]

N_Formimino_L_glutamate + H2O -> N_Formyl_L_glutamate + NH3
(( (E * kcat) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [Cu2+]

H2O + N_Formimino_L_glutamate -> N_Formyl_L_glutamate + NH3
(( (E * C) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant E235A (Zn2+)]

H2O + N_Formimino_L_glutamate -> N_Formyl_L_glutamate + NH3
(( (E * C) ) / (( (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH)))) ) + (( (10 ^ (( (0 - pH) ) ^ 2)) ) / (10 ^ (( (0 - pKa) ) ^ 2))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant E235Q (Zn2+)]

H2O + N_Formimino_L_glutamate -> NH3 + N_Formyl_L_glutamate
(( (E * C) ) / (( (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH)))) ) + (( (10 ^ (( (0 - pH) ) ^ 2)) ) / (10 ^ (( (0 - pKa) ) ^ 2))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [Cd2+]

N_Formimino_L_glutamate + H2O -> NH3 + N_Formyl_L_glutamate
(( (E * C) ) / (( (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH)))) ) + (( (10 ^ (( (0 - pKb) ) ^ 2)) ) / (10 ^ (( (0 - pH) ) ^ 2))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant E235Q (Zn2+)]

N_Formimino_L_glutamate + H2O -> N_Formyl_L_glutamate + NH3
(( (E * kcat) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [formimidoylglutamate deiminase(Enzyme) mutant E235A (Zn2+)]

N_Formimino_L_glutamate + H2O -> NH3 + N_Formyl_L_glutamate
(( (E * kcat) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30
uniprot:Q9HU77 [Ni2+]

N_Formimino_L_glutamate + H2O -> NH3 + N_Formyl_L_glutamate
(( (E * C) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) buffer: 100 mM MES (pH 6.1-6.5) / 100 mM PIPES (pH 6.7-7.3) / 100 mM HEPES (pH 7.3-8.3)/ 100 mM TAPS (pH 8.3-8.9)/ 100 mM CHES (pH 8.9-9.5)
PH: 6.1
Temperature: 30

Pathways

pathway id name