EC: 3.5.1.4

amidase (acylamide amidohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P11436 [Hydroxylamine]

H2O + Acetamide -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acetamide -> Acetate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) wildtype]

Acrylamide + H2O -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) wildtype]

Acrylamide + H2O -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 5 mM alpha-ketoglutarate, 0.3 mM NADH, 2 U/ml glutamate dehydrogenase
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) wildtype]

Acrylamide + H2O -> NH3 + Acrylate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) wildtype]

H2O + Acrylamide -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM potassium phosphate, 5 mM alpha-ketoglutarate, 0.3 mM NADH, 2 U/ml glutamate dehydrogenase
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) wildtype]

Acetamide + H2O -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) mutant T103I]

H2O + Acetamide -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acrylamide -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acrylamide -> NH3 + Acrylate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acrylamide -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acetamide -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

Acetamide + H2O -> Acetate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

Acetamide + H2O -> Acetate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

Acetamide + H2O -> Acetate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) mutant T103I]

Acrylamide + H2O -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) mutant C91A]

Acetamide + H2O -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

Acrylamide + H2O -> NH3 + Acrylate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acetamide -> Acetate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) wildtype]

Acetamide + H2O -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + 4_Nitroacetanilide -> 4_Nitroaniline + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + 4_Nitroacetanilide -> 4_Nitroaniline + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + 4_Nitroacetanilide -> Acetate + 4_Nitroaniline
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + 4_Nitroacetanilide -> Acetate + 4_Nitroaniline
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) wildtype]

H2O + 4_Nitroacetanilide -> Acetate + 4_Nitroaniline
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) mutant T103I]

4_Nitroacetanilide + H2O -> 4_Nitroaniline + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

Acrylamide + H2O -> NH3 + Acrylate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acrylamide -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acetamide -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acetamide -> Acetate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acetamide -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

Acetamide + H2O -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

Acetamide + H2O -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) mutant T103I]

Acetamide + H2O -> Acetate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) mutant C91A]

Acetamide + H2O -> NH3 + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + Acrylamide -> NH3 + Acrylate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

Acrylamide + H2O -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) mutant T103I]

H2O + Acrylamide -> Acrylate + NH3
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

4_Nitroacetanilide + H2O -> Acetate + 4_Nitroaniline
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + 4_Nitroacetanilide -> Acetate + 4_Nitroaniline
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

H2O + 4_Nitroacetanilide -> Acetate + 4_Nitroaniline
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [Hydroxylamine]

4_Nitroacetanilide + H2O -> 4_Nitroaniline + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) wildtype]

H2O + 4_Nitroacetanilide -> 4_Nitroaniline + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:P11436 [amidase(Enzyme) mutant T103I]

H2O + 4_Nitroacetanilide -> 4_Nitroaniline + Acetate
(( (V * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM EDTA, 1 mM beta-mercaptoethanol
PH: 7.2
Temperature: 25
uniprot:Q90578 [amidase(Enzyme) wildtype]

Propionamide + H2O -> Propanoate + NH3
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 25
- [amidase(Enzyme) wildtype]

Anandamide + H2O -> Ethanolamine + Arachidonate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM TrisHCl
PH: 9
Temperature: 37
- [amidase(Enzyme) wildtype]

Anandamide + H2O -> Arachidonate + Ethanolamine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM TrisHCl
PH: 9
Temperature: 37
- [amidase(Enzyme) wildtype]

Anandamide + H2O -> Arachidonate + Ethanolamine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM TrisHCl
PH: 9
Temperature: 37
- [amidase(Enzyme) wildtype]

H2O + Anandamide -> Arachidonate + Ethanolamine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM TrisHCl
PH: 9
Temperature: 37
- [amidase(Enzyme) wildtype]

L_Threoninamide + Hydroxylamine -> NH3 + L_Threoninoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + L_Prolinamide -> L_Prolinoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

D_Lactamide + Hydroxylamine -> NH3 + D_Lactoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

L_Phenylalaninamide + Hydroxylamine -> NH3 + L_Phenylalaninoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

Phenylalaninamide + Hydroxylamine -> NH3 + Phenylalaninoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

Methioninamide + Hydroxylamine -> Methioninoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + D_Alaninamide -> D_Alaninoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Lactamide -> NH3 + Lactoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Leucinamide -> NH3 + Leucinoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

L_Lactamide + Hydroxylamine -> L_Lactoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Nicotinamide -> Nicotinoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Benzamide + Hydroxylamine -> NH3 + Benzoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

L_Alaninamide + Hydroxylamine -> NH3 + L_Alaninoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

L_Leucinamide + Hydroxylamine -> L_Leucinoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

L_Methioninamide + Hydroxylamine -> NH3 + L_Methioninoylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 8
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Isonicotinamide -> Isonicotinoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Isonicotinamide -> Isonicotinoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Metachrylamide -> NH3 + Metachrylylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Acetamide -> NH3 + Acetohydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Pentanamide + Hydroxylamine -> Valerylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Acrylamide -> Acryloylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Hexanoamide -> Hexanoylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Butyramide -> Butyrylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Hydroxylamine + Propionamide -> NH3 + Propionylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Isobutyramide + Hydroxylamine -> Isobutyrylhydroxamic_acid + NH3
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22
- [amidase(Enzyme) wildtype]

Pivalamide + Hydroxylamine -> NH3 + Pivalylhydroxamic_acid
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (A * B) ) + (Kma * B)) ) + (Kmb * A))) buffer: 20 mM phosphate
PH: 7
Temperature: 22

Pathways

pathway id name
BioCyc:META_PWY-581 indole-3-acetate biosynthesis II
BioCyc:YEAST_PWY3O-4107 NAD salvage pathway
BioCyc:ARA_PWY-5381 pyridine nucleotide cycling (plants)
BioCyc:MTBCDC1551_PWY-7308 acrylonitrile degradation I
BioCyc:MTBCDC1551_PYRIDNUCSAL-PWY NAD salvage pathway I
BioCyc:MTBCDC1551_ARGDEG-V-PWY L-arginine degradation X (arginine monooxygenase pathway)
BioCyc:META_P345-PWY aldoxime degradation
BioCyc:ARA_PWY-581 indole-3-acetate biosynthesis II
PlantCyc:ARA_PWY-5381 pyridine nucleotide cycling (plants)
PlantCyc:PLANT_PWY-5381 pyridine nucleotide cycling (plants)
PlantCyc:ARA_PWY3O-4107 NAD salvage pathway V (PNC V cycle)
PlantCyc:PLANT_PWY-581 indole-3-acetate biosynthesis II
PlantCyc:ARA_PWY-581 indole-3-acetate biosynthesis II