EC: 3.5.1.16

acetylornithine deacetylase (N2-acetyl-L-ornithine amidohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P23908 [Co2+]

N2_Acetyl_L_ornithine + H2O -> L_Ornithine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + (A * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

N2_Acetyl_L_ornithine + H2O -> Acetate + L_Ornithine		 (( (( (kcat * E) ) * A) ) / (( (Km * (1 + (I / Kis))) ) + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

H2O + N2_Acetyl_L_ornithine -> Acetate + L_Ornithine		 (( (( (kcat * E) ) * A) ) / (( (Km * (1 + (I / Kis))) ) + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

N_Formyl_L_methionine + H2O -> L_Methionine + Formate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

H2O + N_Acetyl_L_leucine -> L_Leucine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

N_Acetyl_L_lysine + H2O -> L_Lysine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

N_Acetyl_L_methionine + H2O -> Acetate + L_Methionine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

N_Acetyl_DL_serine + H2O -> Acetate + Serine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

H2O + N_Chloroacetylglycine -> Glycine + Chloroacetic_acid		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Mg2+]

H2O + N2_Acetyl_L_ornithine -> Acetate + L_Ornithine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [acetylornithine deacetylase(Enzyme) wildtype]

H2O + N2_Acetyl_L_ornithine -> L_Ornithine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

H2O + N2_Acetyl_L_ornithine -> L_Ornithine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Cd2+]

N2_Acetyl_L_ornithine + H2O -> L_Ornithine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Ni2+]

H2O + N2_Acetyl_L_ornithine -> Acetate + L_Ornithine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

N_Acetyl_L_cysteine + H2O -> Acetate + L_Cysteine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

N_Acetyl_L_alanine + H2O -> L_Alanine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

N_Acetyl_L_asparagine + H2O -> L_Asparagine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [Co2+]

H2O + N_Acetyl_L_glutamine -> L_Glutamine + Acetate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25
uniprot:P23908 [acetylornithine deacetylase(Enzyme) wildtype]

H2O + N2_Acetyl_L_ornithine -> Acetate + L_Ornithine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K2HPO4 (Chelex 100 treated)
PH: 7.5
Temperature: 25
uniprot:P23908 [Co2+]

N2_Acetyl_L_ornithine + H2O -> L_Ornithine + Acetate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K2HPO4 (Chelex 100 treated)
PH: 7.5
Temperature: 25
uniprot:P23908 [acetylornithine deacetylase(Enzyme) wildtype]

H2O + N2_Acetyl_L_ornithine -> Acetate + L_Ornithine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:ECO_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ARA_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:VCHO_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:ECOO157_GLUTORN-PWY ornithine biosynthesis
BioCyc:ECOL316407_GLUTORN-PWY ornithine biosynthesis
BioCyc:ECOL316407_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:ECO_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:ECO_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:META_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:META_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ARA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:ECOL316407_ARGSYN-PWY arginine biosynthesis I (via L-ornithine)
BioCyc:META_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:VCHO_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ECOO157_ARGSYN-PWY arginine biosynthesis I (via L-ornithine)
PathBank:SMP0000812 Arginine Metabolism
PathBank:SMP0000813 Ornithine Metabolism
PlantCyc:ARA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
PlantCyc:ARA_GLUTORN-PWY L-ornithine biosynthesis I
PathBank:SMP0002420 Proline Metabolism
PathBank:SMP0002416 Arginine Metabolism